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Ltk (NM_008523) Mouse Tagged ORF Clone
CAT＃: MG222731
- TrueORF®
Ltk (tGFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_008523" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222731 representing NM_008523
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGAGACCCGCGCGGCGGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGCGGTGATACTTCTGAGT CTGACCTCCTCTGGGCTGATGGGGAAGATGGCACATCCTTTGTCCACCCCAGTGGTGAGCTCTACCTACA GCCTCTGGCAGTCACAGAGGGCCATGGGGAGGTGGAGATCCGAAAGCATCCCAACTGCAGTCACTGCCCT TTCAAAGACTGCCAGTGGCAGGCAGAGCTCTGGACGGCCGAATGCACGTGCCCAGAGGGCACGGAGCTAG CTGTGGATAATGTCACTTGCATGGACCTGCCAACCACCGCAAGCCCTCTGATCCTGATGGGAGCTGTAGT GGCAGCCTTGGCACTGAGTCTCCTAATGATGTGTGCAGTCCTGATTCTAGTGAACCAGAAGTGTCAGGGC CTGTGGGGGACCAGGCTGCCAGGCCCTGAGCTTGAGCTAAGCAAGCTTCGATCCTCTGCCATCAGGACAG CACCCAACCCTTACTATTGTCAGGTGGGACTCAGTCCTGCCCAGCCCTGGCCTTTGCCCCCAGGGCTCAC TGAGGTTTCACCAGCCAATGTCACTCTACTCAGAGCCCTTGGCCATGGTGCCTTTGGGGAAGTGTACGAG GGACTAGTGACTGGTCTTCCTGGGGACTCCAGTCCTCTTCCAGTGGCTATTAAGACTCTGCCAGAGCTCT GCTCCCATCAGGATGAGCTGGATTTTCTCATGGAGGCTCTGATCATCAGCAAGTTCAGCCATCAGAACAT TGTACGCTGTGTGGGGCTCAGCTTTCGGTCTGCCCCGCGCCTCATTCTGCTGGAGCTGATGTCTGGTGGG GACATGAAGAGCTTTTTGAGGCACAGCAGACCACACCCAGGACAACTGGCACCTCTGACCATGCAGGACC TATTGCAGCTGGCCCAGGATATAGCCCAGGGCTGCCACTACCTGGAGGAAAATCACTTCATTCACAGAGA CATTGCTGCCCGTAACTGTCTGCTTAGCTGCAGTGGAGCCAGCCGAGTGGCCAAGATTGGAGATTTTGGA ATGGCAAGAGATATCTACCAGGCCAGTTATTATCGCAAGGGTGGCCGGACCTTGCTCCCAGTCAAGTGGA TGCCGCCAGAAGCTCTCCTGGAGGGCCTTTTCACATCCAAGACAGACTCCTGGTCTTTTGGGGTCCTGCT CTGGGAGATCTTCTCACTGGGGTATATGCCCTACCCTGGACATACCAACCAGGAGGTTCTAGACTTCATT GCCACAGGGAACAGGATGGACCCTCCTAGGAACTGTCCTGGGCCAGTGTACCGAATCATGACCCAGTGTT GGCAGCATCAGCCGGAGCTCCGCCCTGACTTTGGCAGCATCTTGGAACGGATTCAGTACTGCACTCAGGA CCCTGATGTGCTGAACTCACCCCTGCCCGTGGAACCTGGGCCCATTCTAGAGGAGGAAGAGGCCTCCAGG CTGGGAAACAGGTCACTGGAGGGTCTTAGATCCCCAAAGCCCCTAGAGCTGAGTTCTCAGAACTTGAAGA GCTGGGGAGGAGGCCTTCTTGGCTCTTGGCTGCCCTCTGGCCTCAAGACCCTCAAACCCAGGTGCCTCCA ACCTCAGAACATTTGGAACCCCACCTATGGCTCCTGGACCCCAAGGGGCCCCCAGGGTGAAGATACAGGC ATTGAACACTGCAATGGCTCCTCCTCAAGTTCCATTCCAGGCATCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222731 representing NM_008523
Red=Cloning site Green=Tags(s) LETRAAAPGSGGRGGAAGGDTSESDLLWADGEDGTSFVHPSGELYLQPLAVTEGHGEVEIRKHPNCSHCP FKDCQWQAELWTAECTCPEGTELAVDNVTCMDLPTTASPLILMGAVVAALALSLLMMCAVLILVNQKCQG LWGTRLPGPELELSKLRSSAIRTAPNPYYCQVGLSPAQPWPLPPGLTEVSPANVTLLRALGHGAFGEVYE GLVTGLPGDSSPLPVAIKTLPELCSHQDELDFLMEALIISKFSHQNIVRCVGLSFRSAPRLILLELMSGG DMKSFLRHSRPHPGQLAPLTMQDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCSGASRVAKIGDFG MARDIYQASYYRKGGRTLLPVKWMPPEALLEGLFTSKTDSWSFGVLLWEIFSLGYMPYPGHTNQEVLDFI ATGNRMDPPRNCPGPVYRIMTQCWQHQPELRPDFGSILERIQYCTQDPDVLNSPLPVEPGPILEEEEASR LGNRSLEGLRSPKPLELSSQNLKSWGGGLLGSWLPSGLKTLKPRCLQPQNIWNPTYGSWTPRGPQGEDTG IEHCNGSSSSSIPGIQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008523 |
| ORF Size | 1728 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008523.2, NP_032549.2 |
| RefSeq Size | 2269 bp |
| RefSeq ORF | 1731 bp |
| Locus ID | 17005 |
| Gene Summary | The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219165 | Ltk (untagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 1, (10ug) |
CNY 7,320.00 |
|
| MR222731 | Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 1 |
CNY 4,288.00 |
|
| MR222731L3 | Lenti ORF clone of Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 1 |
CNY 6,460.00 |
|
| MR222731L4 | Lenti ORF clone of Ltk (mGFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 1 |
CNY 6,460.00 |
