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Col4a1 (NM_009931) Mouse Tagged ORF Clone
CAT＃: MG222881
- TrueORF®
Col4a1 (tGFP-tagged) - Mouse collagen type IV alpha 1 (Col4a1), (10ug)
ORF Plasmid: DDK
"NM_009931" in other vectors (2)
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CNY 18,424.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Br; Bru; Col4; Col4a-1; Del(8)44; R; Raw; S; Svc |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222881 representing NM_009931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGCGTGAAGGGACA AAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGCACAAAAGGGACGAGGG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAAGATGGCCCTCC AGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGCCGCTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGGAGATCCTGGTGAAATTCTCG GCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATCCCCGGGATGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACCAAAAGGTGACAAGGGAGAGC AAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGAAAGGGGGAGCCCGGGCATTC CTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGAGAGAAGGGTGAAGCTGGACT TCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAGGAGATCGAGGCTACCCAGGA GCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACCAGGCCAACCAGGCCCTCCAG GTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGGGGAGAAAAAGGTGACCAGGG ATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGGGGCCTCCCGGCCCTCCCGGC CCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGGACCACCCGGGGACCAGGGCC CTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGCCTCCGGGAGAGATTGGTTTC CCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCTGGAGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCATGCCAGGAAGAGCAGGAACT CCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGGTTCAATAGGATTGAAAGGAG AGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATCGGCCCTCCTGGACCCCCAGG AGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTGGAGGCCCTGGGTCCCCAGGT CTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCCCCCAGGAGCTGCAGGACTTC CAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGTCCAGGCAT CCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGGATTTAACGGCTTACCTGGCA ACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAACCAGGCCT TCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCGGCGTTCCAGGAGAACAGGGG TTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCCTGGAGTTCAAGGCCCAGCAG GTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG GTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCCCCGGACTGGATATGCCTGGC CCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGCCTCCCTGGCCTTCCTG GACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGTGTCATGGGAACCCC GGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAGAAAAAGGGGACCATGGCCTT CCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGACGTTGGGCTTCCTGGCA TGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAAGGAGACCAGGGAGAGAAAGG ACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACACCAGGAGTACCTGGGAAGGAT GGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCTTAGTGGGACACCAGGATCCC CTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGTTCGCCTGGAGAAAAAGGTGT GCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGGGAGCCAAAGGAGAGAAAGGG CAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGGAGACCAGGGCCTTGCTGGCT TCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAGAAAGGGGATAAA GGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCTCCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAGACAAAGGCTCCAAGGGTGAA GTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGGCGAGCAAGGGTTCATGGGTC CTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGGGGCCGCCAGGGTTCCCAGGA ATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCCGGGGGTTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTCTGCAGGGAGTGAAAGGAGAC CAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCTCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTTAAAGGACTCCAGGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTC GATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGGTCCAAGAGGGTTTCCTGGCC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGA TACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTTGGGTACGGCTGGCAGCTGCC TGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGGCACCCATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGGTGTGCGGTTTGTGAAGCACCGGCCATGGTGATGGCGGTACACAGTCAGA CCATTCAGATTCCGCAGTGCCCTAACGGTTGGTCCTCACTGTGGATCGGCTATTCCTTCGTGATGCACAC CAGCGCTGGTGCTGAAGGTTCCGGCCAAGCCCTCGCATCCCCCGGGTCCTGTCTGGAAGAGTTTAGAAGC GCCCCATTCATCGAGTGCCACGGCAGAGGAACGTGCAATTACTACGCAAATGCTTACAGCTTTTGGCTCG CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCATCCACCTTGAAGGCAGGGGAGCTGCGAAC ACACGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222881 representing NM_009931
Red=Cloning site Green=Tags(s) MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG PHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEP GKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPG APGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPG PPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGT PGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPG LPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGV DGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQG LTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPG PKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGL PGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKD GQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKG QSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDK GLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGE VGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPG INGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGD QGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGF DGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDN IRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009931 |
| ORF Size | 5007 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009931.2, NP_034061.2 |
| RefSeq Size | 6636 bp |
| RefSeq ORF | 5010 bp |
| Locus ID | 12826 |
| UniProt ID | P02463 |
| Gene Summary | This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
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