Col4a1 (NM_009931) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Br; Bru; Col4; Col4a-1; Del(8)44; R; Raw; S; Svc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224806 representing NM_009931 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGCGTGAAGGGACA AAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGCACAAAAGGGACGAGGG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAAGATGGCCCTCC AGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGCCGCTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGGAGATCCTGGTGAAATTCTCG GCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATCCCCGGGATGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACCAAAAGGTGACAAGGGAGAGC AAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGAAAGGGGGAGCCCGGGCATTC CTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGAGAGAAGGGTGAAGCTGGACT TCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAGGAGATCGAGGCTACCCAGGA GCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACCAGGCCAACCAGGCCCTCCAG GTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGGGGAGAAAAAGGTGACCAGGG ATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGGGGCCTCCCGGCCCTCCCGGC CCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGGACCACCCGGGGACCAGGGCC CTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGCCTCCGGGAGAGATTGGTTTC CCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCTGGAGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCATGCCAGGAAGAGCAGGAACT CCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGGTTCAATAGGATTGAAAGGAG AGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATCGGCCCTCCTGGACCCCCAGG AGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTGGAGGCCCTGGGTCCCCAGGT CTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCCCCCAGGAGCTGCAGGACTTC CAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGTCCAGGCAT CCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGGATTTAACGGCTTACCTGGCA ACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAACCAGGCCT TCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCGGCGTTCCAGGAGAACAGGGG TTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCCTGGAGTTCAAGGCCCAGCAG GTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG GTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCCCCGGACTGGATATGCCTGGC CCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGCCTCCCTGGCCTTCCTG GACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGTGTCATGGGAACCCC GGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAGAAAAAGGGGACCATGGCCTT CCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGACGTTGGGCTTCCTGGCA TGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAAGGAGACCAGGGAGAGAAAGG ACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACACCAGGAGTACCTGGGAAGGAT GGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCTTAGTGGGACACCAGGATCCC CTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGTTCGCCTGGAGAAAAAGGTGT GCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGGGAGCCAAAGGAGAGAAAGGG CAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGGAGACCAGGGCCTTGCTGGCT TCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAGAAAGGGGATAAA GGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCTCCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAGACAAAGGCTCCAAGGGTGAA GTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGGCGAGCAAGGGTTCATGGGTC CTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGGGGCCGCCAGGGTTCCCAGGA ATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCCGGGGGTTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTCTGCAGGGAGTGAAAGGAGAC CAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCTCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTTAAAGGACTCCAGGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTC GATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGGTCCAAGAGGGTTTCCTGGCC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGA TACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTTGGGTACGGCTGGCAGCTGCC TGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGGCACCCATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGGTGTGCGGTTTGTGAAGCACCGGCCATGGTGATGGCGGTACACAGTCAGA CCATTCAGATTCCGCAGTGCCCTAACGGTTGGTCCTCACTGTGGATCGGCTATTCCTTCGTGATGCACAC CAGCGCTGGTGCTGAAGGTTCCGGCCAAGCCCTCGCATCCCCCGGGTCCTGTCTGGAAGAGTTTAGAAGC GCCCCATTCATCGAGTGCCACGGCAGAGGAACGTGCAATTACTACGCAAATGCTTACAGCTTTTGGCTCG CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCATCCACCTTGAAGGCAGGGGAGCTGCGAAC ACACGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009931
Insert Size	5010 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009931.2, NP_034061.2
RefSeq Size	6636 bp
RefSeq ORF	5010 bp
Locus ID	12826
UniProt ID	P02463
Gene Summary	This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein.

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