Col18a1 (NM_001109991) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	endostatin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG222900 representing NM_001109991 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGACCCCAGCAGACGCCTCTGCCTGCTGCTGCTGTTGCTGCTCTCCTGCCGCCTTGTGCCTG CCAGCGCTGATGGGAATTCCCTGAGTCCCCTGAATCCCCTGGTGTGGCTCTGGCCGCCCAAGACCTCTGA TTCTTTGGAAGGCCCGGTCTCGAAACCCCAGAACAGTTCACCTGTGCAGTCCACAGAGAACCCCACCACG CACGTGGTCCCCCAGGATGGCCTCACAGAACAACAGACGACTCCTGCCAGCTCCGAGCTGCCCCCGGAGG AAGAGGAGGAGGAGGATCAGAAAGCTGGCCAGGGTGGCAGCCCTGCAACCCCTGCTGTCCCCATTCCCCT GGTGGCCCCTGCTGCGAGCCCAGACATGAAGGAGGAGAATGTTGCGGGCGTTGGAGCCAAGATTCTGAAT GTGGCTCAGGGCATTCGGAGCTTCGTCCAGCTGTGGGATGAGGACTCGACTATTGGGCACTCTGCTGGGA CTGAGGTTCCAGATTCTTCAATCCCTACAGTCCTCCCCTCTCCTGCCGAGCTCTCCAGTGCTCCCCAGGG GAGTAAGACCACTCTCTGGCTAAGCAGTGCCATTCCAAGTTCTCCAGATGCTCAGACAACTGAGGCTGGC ACGCTGGCTGTGCCCACCCAGCTGCCTCCCTTCCAGAGCAACCTCCAGGCACCACTGGGAAGACCCTCAG CCCCACCAGACTTCCCAGAGAATGTTGCTGAGGAGGTGGGGCTGCTGCAGCTCCTTGGAGACCCCCTACC TGAGAAGATCTCACAAATCGATGACCCTCACGTCGGGCCGGCCTACATCTTTGGACCAGACTCCAACAGT GGCCAGGTGGCCCAGTATCATTTCCCAAAACTCTTCTTCCGGGACTTTTCGCTGCTGTTTCATGTCCGGC CAGCCACAGAGGCAGCAGGGGTGCTATTTGCCATCACAGATGCTGCCCAGGTGGTAGTCTCACTGGGCGT GAAGCTCTCAGAGGTCCGAGATGGACAGCAAAACATCTCATTGCTCTACACGGAGCCTGGGGCCAGCCAG ACCCAGACGGGAGCCAGCTTCCGCCTACCTGCATTTGTTGGGCAGTGGACACACTTCGCGCTCAGCGTCG ACGGAGGCTCTGTGGCTCTCTACGTAGACTGTGAAGAATTCCAGAGGGTGCCATTTGCTCGGGCCTCGCA GGGACTGGAGCTAGAGCGTGGCGCTGGCCTCTTTGTGGGTCAGGCTGGAACAGCAGACCCTGACAAGTTC CAGGGGATGATCTCAGAGCTGAAGGTACGCAAAACCCCCCGGGTGAGCCCTGTGCACTGTCTGGATGAAG AAGATGATGATGAAGACCGGGCATCTGGAGATTTTGGAAGTGGCTTTGAAGAAAGCAGCAAGTCACACAA GGAGGATACATCTCTACTACCTGGGCTCCCTCAGCCACCTCCTGTCACTTCCCCACCCCTGGCTGGAGGC AGCACCACAGAAGATCCTAGAACAGAAGAAACGGAGGAAGACGCCGCGGTAGATTCTATAGGAGCTGAGA CCCTTCCTGGCACAGGTTCAAGCGGTGCATGGGATGAGGCTATCCAGAACCCCGGAAGGGGCTTGATAAA GGGAGGTATGAAAGGACAAAAGGGAGAACCAGGTGCCCAGGGCCCACCTGGCCCAGCTGGGCCCCAGGGT CCTGCCGGTCCAGTGGTCCAGAGCCCCAACTCACAACCTGTCCCTGGAGCACAAGGACCCCCGGGACCTC AGGGGCCACCAGGGAAGGATGGCACTCCAGGAAGGGATGGTGAACCGGGTGACCCTGGTGAAGATGGGAG ACCGGGTGACACTGGACCTCAAGGCTTTCCAGGGACCCCAGGAGATGTGGGCCCTAAGGGCGAGAAGGGA GATCCTGGTATTGGGCCCCGAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCCTCCTTCAGACAAGACA AGCTGACCTTCATTGACATGGAGGGATCCGGTTTCAGCGGAGACATAGAGAGCCTTAGAGGCCCACGAGG CTTCCCTGGCCCCCCGGGGCCCCCTGGTGTCCCAGGACTTCCTGGTGAGCCAGGACGCTTTGGGATCAAT GGTTCCTATGCACCAGGACCTGCAGGCCTTCCTGGTGTACCTGGGAAGGAAGGACCCCCCGGTTTTCCAG GTCCCCCGGGACCTCCAGGTCCTCCAGGCAAAGAGGGCCCACCAGGAGTGGCCGGCCAGAAAGGCAGTGT TGGTGATGTGGGCATCCCAGGACCCAAGGGGAGCAAAGGAGACCTTGGGCCCATCGGTATGCCTGGCAAG TCTGGCTTGGCTGGATCCCCTGGGCCAGTTGGACCCCCAGGACCTCCAGGGCCTCCAGGGCCACCAGGAC CAGGATTTGCTGCTGGATTCGATGATATGGAAGGCTCTGGAATACCCCTCTGGACAACAGCCCGAAGCTC TGATGGGCTGCAGGGACCTCCCGGGTCGCCGGGACTCAAGGGGGATCCTGGAGTGGCAGGCCTACCTGGA GCCAAGGGAGAAGTTGGAGCAGATGGAGCCCAGGGCATCCCTGGTCCCCCAGGAAGAGAAGGTGCAGCTG GATCTCCGGGGCCAAAAGGAGAGAAAGGGATGCCGGGAGAAAAGGGAAACCCAGGAAAAGATGGAGTGGG CCGGCCGGGCCTCCCTGGGCCTCCAGGACCTCCAGGGCCTGTGATCTATGTGTCAAGTGAGGATAAAGCA ATAGTGAGCACGCCAGGACCTGAGGGCAAGCCAGGGTACGCAGGCTTTCCTGGACCTGCTGGACCGAAGG GTGACCTGGGTTCCAAAGGCGAGCAGGGTCTTCCGGGGCCCAAGGGTGAGAAGGGAGAGCCAGGCACTAT CTTTAGTCCTGATGGCAGAGCTCTGGGCCATCCCCAGAAGGGAGCCAAGGGAGAGCCAGGCTTTCGAGGA CCCCCGGGTCCTTATGGACGACCTGGGCACAAGGGTGAAATTGGCTTCCCTGGACGGCCGGGTCGACCTG GAACGAATGGCTTAAAGGGAGAGAAAGGAGAGCCTGGAGATGCCAGCCTTGGGTTCAGCATGAGGGGATT GCCTGGCCCCCCTGGGCCTCCAGGACCCCCAGGTCCTCCTGGGATGCCCATCTATGACAGCAATGCATTT GTGGAGTCTGGCCGACCTGGACTACCAGGACAGCAGGGTGTGCAGGGGCCTTCAGGACCAAAGGGTGACA AAGGAGAGGTGGGCCCACCTGGGCCACCAGGGCAATTCCCCATTGACCTCTTCCACCTGGAAGCGGAAAT GAAGGGGGACAAGGGAGACCGAGGGGATGCTGGACAGAAAGGAGAGAGGGGAGAACCTGGGGCTCCTGGT GGTGGATTCTTCAGCTCAAGTGTACCTGGCCCCCCCGGCCCACCTGGATACCCTGGAATTCCGGGTCCAA AGGGAGAGAGCATCCGGGGGCCACCTGGCCCTCCTGGCCCGCAGGGACCTCCTGGCATTGGCTATGAGGG TCGCCAGGGTCCCCCAGGACCTCCGGGACCTCCAGGACCTCCCTCCTTCCCTGGCCCTCACAGACAGACT GTCAGTGTTCCTGGTCCTCCGGGCCCACCTGGTCCTCCAGGTCCCCCAGGAGCCATGGGTGCCTCTGCTG GGCAGGTGAGGATCTGGGCCACATACCAGACCATGCTGGACAAGATCCGGGAGGTGCCGGAGGGCTGGCT CATCTTTGTGGCCGAGAGGGAAGAGCTCTATGTACGCGTTAGAAATGGCTTCCGGAAGGTGCTGCTGGAG GCCCGGACAGCCCTCCCGAGAGGCACGGGCAATGAGGTGGCTGCTTTGCAGCCCCCATTGGTCCAGCTTC ATGAGGGCAGTCCATACACCCGGAGGGAGTACTCCTATTCCACGGCACGACCCTGGCGAGCAGATGACAT CCTGGCCAACCCACCGCGCCTGCCAGACCGCCAGCCTTACCCCGGAGTTCCACATCACCACAGTTCCTAT GTGCACCTGCCGCCAGCCCGCCCCACCCTCTCACTTGCTCATACTCATCAGGACTTTCAGCCAGTGCTCC ACCTGGTGGCACTGAACACCCCCCTGTCTGGAGGCATGCGTGGTATCCGTGGAGCAGATTTCCAGTGCTT CCAGCAAGCCCGAGCCGTGGGGCTGTCGGGCACCTTCCGGGCTTTCCTGTCCTCTAGGCTGCAGGATCTC TATAGCATCGTGCGCCGTGCTGACCGGGGGTCTGTGCCCATCGTCAACCTGAAGGACGAGGTGCTATCTC CCAGCTGGGACTCCCTGTTTTCTGGCTCCCAGGGTCAACTGCAACCCGGGGCCCGCATCTTTTCTTTTGA CGGCAGAGATGTCCTGAGACACCCAGCCTGGCCGCAGAAGAGCGTATGGCACGGCTCGGACCCCAGTGGG CGGAGGCTGATGGAGAGTTACTGTGAGACATGGCGAACTGAAACTACTGGGGCTACAGGTCAGGCCTCCT CCCTGCTGTCAGGCAGGCTCCTGGAACAGAAAGCTGCGAGCTGCCACAACAGCTACATCGTCCTGTGCAT TGAGAATAGCTTCATGACCTCTTTCTCCAAA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG222900 representing NM_001109991 Red=Cloning site Green=Tags(s) MAPDPSRRLCLLLLLLLSCRLVPASADGNSLSPLNPLVWLWPPKTSDSLEGPVSKPQNSSPVQSTENPTT HVVPQDGLTEQQTTPASSELPPEEEEEEDQKAGQGGSPATPAVPIPLVAPAASPDMKEENVAGVGAKILN VAQGIRSFVQLWDEDSTIGHSAGTEVPDSSIPTVLPSPAELSSAPQGSKTTLWLSSAIPSSPDAQTTEAG TLAVPTQLPPFQSNLQAPLGRPSAPPDFPENVAEEVGLLQLLGDPLPEKISQIDDPHVGPAYIFGPDSNS GQVAQYHFPKLFFRDFSLLFHVRPATEAAGVLFAITDAAQVVVSLGVKLSEVRDGQQNISLLYTEPGASQ TQTGASFRLPAFVGQWTHFALSVDGGSVALYVDCEEFQRVPFARASQGLELERGAGLFVGQAGTADPDKF QGMISELKVRKTPRVSPVHCLDEEDDDEDRASGDFGSGFEESSKSHKEDTSLLPGLPQPPPVTSPPLAGG STTEDPRTEETEEDAAVDSIGAETLPGTGSSGAWDEAIQNPGRGLIKGGMKGQKGEPGAQGPPGPAGPQG PAGPVVQSPNSQPVPGAQGPPGPQGPPGKDGTPGRDGEPGDPGEDGRPGDTGPQGFPGTPGDVGPKGEKG DPGIGPRGPPGPPGPPGPSFRQDKLTFIDMEGSGFSGDIESLRGPRGFPGPPGPPGVPGLPGEPGRFGIN GSYAPGPAGLPGVPGKEGPPGFPGPPGPPGPPGKEGPPGVAGQKGSVGDVGIPGPKGSKGDLGPIGMPGK SGLAGSPGPVGPPGPPGPPGPPGPGFAAGFDDMEGSGIPLWTTARSSDGLQGPPGSPGLKGDPGVAGLPG AKGEVGADGAQGIPGPPGREGAAGSPGPKGEKGMPGEKGNPGKDGVGRPGLPGPPGPPGPVIYVSSEDKA IVSTPGPEGKPGYAGFPGPAGPKGDLGSKGEQGLPGPKGEKGEPGTIFSPDGRALGHPQKGAKGEPGFRG PPGPYGRPGHKGEIGFPGRPGRPGTNGLKGEKGEPGDASLGFSMRGLPGPPGPPGPPGPPGMPIYDSNAF VESGRPGLPGQQGVQGPSGPKGDKGEVGPPGPPGQFPIDLFHLEAEMKGDKGDRGDAGQKGERGEPGAPG GGFFSSSVPGPPGPPGYPGIPGPKGESIRGPPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQT VSVPGPPGPPGPPGPPGAMGASAGQVRIWATYQTMLDKIREVPEGWLIFVAEREELYVRVRNGFRKVLLE ARTALPRGTGNEVAALQPPLVQLHEGSPYTRREYSYSTARPWRADDILANPPRLPDRQPYPGVPHHHSSY VHLPPARPTLSLAHTHQDFQPVLHLVALNTPLSGGMRGIRGADFQCFQQARAVGLSGTFRAFLSSRLQDL YSIVRRADRGSVPIVNLKDEVLSPSWDSLFSGSQGQLQPGARIFSFDGRDVLRHPAWPQKSVWHGSDPSG RRLMESYCETWRTETTGATGQASSLLSGRLLEQKAASCHNSYIVLCIENSFMTSFSK SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001109991
ORF Size	4581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001109991.1, NP_001103461.1
RefSeq Size	5642 bp
RefSeq ORF	4584 bp
Locus ID	12822
UniProt ID	P39061
Gene Summary	Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.[UniProtKB/Swiss-Prot Function]

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