Tnnt2 (NM_011619) Mouse Tagged ORF Clone
CAT#: MG223334
- TrueORF®
Tnnt2 (tGFP-tagged) - Mouse troponin T2 cardiac (Tnnt2) transcript variant 9, (10ug)
ORF Plasmid: DDK
"NM_011619" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | cTnT; Tnt |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223334 representing NM_011619
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAGCAAGAGGAGGCAGTGGAGG AGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGCCAACGTAGAAGA GGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAACCCAAGCCCAGC AGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACTTTGATGACATCC ACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTTCGAGAACAGGAA GAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAGCGGGCCGAGCAG CAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGGCCCGGCGTGAGG AGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAACATGATGCACTT TGGAGGGTACATCCAGAAGACAGAGCGGAAGAGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAG ATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCA AGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCA GAAATACGAAATCAACGTTCTGCGAAACCGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAG GCCAAAGTCACCGGGCGTTGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223334 representing NM_011619
Red=Cloning site Green=Tags(s) MSDAEEVVEEYEEEQEEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEGPVEDTKPKPS RLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAERAEQ QRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKK ILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGK AKVTGRWK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011619 |
ORF Size | 864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011619.3, NP_035749.1 |
RefSeq Size | 1160 bp |
RefSeq ORF | 867 bp |
Locus ID | 21956 |
UniProt ID | P50752 |
Gene Summary | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209565 | Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 9, (10ug) |
CNY 3,990.00 |
|
MR223334 | Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 9 |
CNY 2,850.00 |
|
MR223334L3 | Lenti ORF clone of Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 9 |
CNY 4,750.00 |
|
MR223334L4 | Lenti ORF clone of Tnnt2 (mGFP-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 9 |
CNY 4,750.00 |