G2e3 (NM_001167964) Mouse Tagged ORF Clone
CAT#: MG223648
- TrueORF®
G2e3 (tGFP-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001167964" in other vectors (4)
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CNY 6,840.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6030408C04Rik; 9030416F18; AW046379; D930034K21Rik; mKIAA1333 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223648 representing NM_001167964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGAAAATAAACCTGATAATTCACAAAGTCTTGCTTGTGTTTTCTGCCGAAAAAATGATGACTGTC CCAATAAATACGGAGAAAAGAAAACATATGAAAAATGGAATTTTAGTGTCCACTACTACTGCTTGTTAAT GTCAAGTGGAATTTGGCAGAGAGGTAAAGAAGAAGAAGGAGTTTATGGCTTCCTAATAGAAGACATCAGG AAGGAAGTCCAGAGAGCCTCCAAACTGAAGTGTACAGTTTGCAAGAAGAATGGTGCTTCAATTGGATGTG TGGTACCCACGTGTAAACGAAGTTATCACTTGCCTTGTGGACTTCAGAAAGAATGTATTTTCCAGTTCAC TGACAATTTTGCGTCATTTTGCTGGAAACATCGACCCGTTCAAGCAATTACATCTAATAAATATAGTAGT TCTTTACCATGTACTATCTGTTTGGAATTTGTCGAGCCTATTCCTACTTATAACATACTACAAAGTCCCT GTTGTAAGAATGCTTGGTTTCATAGAGACTGTTTACAGGTCCAAGCAATAAATGCAGGAGTGTTTTTCTT TAGATGTACACTATGCAATAATACAGACATCTTTCAGAAGGAGATGTTGAGAATGGGAATTCATATTCCT GAAAAAGATGCATCCTGGGAATTAGAAGAAAACGCGTATCAAGAGCTGCTGCAGAGCCACGACCGCTGTG ATATTCGAAGATGTCACTGCAAAAAAGGCCGAGACTACAACGAACCTAACAGCAAATGGGAAGTAAAGCG GTGTCAGAGTTGTGGTTCTAGTGGAACACACTTAGCCTGTTCCTCACTACAGTCTTGGGAACAAAATTGG GAGTGTTTGGACTGTAGAAGAATTACCTACACTTCAGATTTCCAAAAGGCCCCCAAACACCCATTAGCCA ACTCTACTAATGTGACAGTTACTGACTGCTTGTTGGAAGAGTCATCATCTAAGCTCCCCAGACAGTCAAC TGTAGCCCAGCATAAAGAACTACTGAGTTTATTTTTATCTGTATTTACAGCTGTGAAAGAGAATCTTTAC TATGAAGTTGGCAAAATGCTTGCCATTTCTTTAGTTCATGGTGGTCCTTCGCCTGGTTTCTTTTCTGAAA CCTTGTTTAACTGCCTTGCTTATGGACCAGAAAATACTCTACCAACTTTGGATGATGTATCAGACATTGA TGTTGCACAGATTATAATCAAGATAGATTCTGCAACTGATTTAAACATTTTAAATTCAGTAATAAGCCAA CACTATAACTACTTAGAGGTCAGTGGATGTTTAAGACTTACGACATCATTAAGTGATAAATTCATGTTGG TAAAAGACATCCTTTTCTACCATGTAATTAATAGAGTCAAGGCACCTTTTGAGAGTTTTAAACAGGGTCT GAAAACGCTTGGTGTTTTAGAGAAAATTCAAACTTACCCAGAAGCATTTTATAAGATCCTATGTCATAAA CCTGAGAATCTCTCTGCAAAAAACCTCAGTGACCTCTTCACAATTCACTCGGTAGCGGACGTACAGACTT TGAGGTTTTGGAACAGTTACTTAAAGGCTATAGAGGATGGTAAATCTGCAACAACGATGGAAGATATTCT TATTTTTGCAACTGGCTGTAGTTCTGTCCCACCAACTGGATTTAAACCCTCTCTGTCAGTTGAATGTCTA CATGTGGATTTTCCTGTTGCAGACAAATATAGAAACCATTTAGTTCTACCAGCCACCAACACGTATGAAG AGTTCCAAGAAAATATGGATTTCACCATAAGAGACACTCTAAGATTAGAAAAGGAAGAAAGGTCTCACAT ACTACCTCGGACATTAAATGTTTCTTCTAATGAAGAAATGCTTATT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223648 representing NM_001167964
Red=Cloning site Green=Tags(s) MNENKPDNSQSLACVFCRKNDDCPNKYGEKKTYEKWNFSVHYYCLLMSSGIWQRGKEEEGVYGFLIEDIR KEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQAITSNKYSS SLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIP EKDASWELEENAYQELLQSHDRCDIRRCHCKKGRDYNEPNSKWEVKRCQSCGSSGTHLACSSLQSWEQNW ECLDCRRITYTSDFQKAPKHPLANSTNVTVTDCLLEESSSKLPRQSTVAQHKELLSLFLSVFTAVKENLY YEVGKMLAISLVHGGPSPGFFSETLFNCLAYGPENTLPTLDDVSDIDVAQIIIKIDSATDLNILNSVISQ HYNYLEVSGCLRLTTSLSDKFMLVKDILFYHVINRVKAPFESFKQGLKTLGVLEKIQTYPEAFYKILCHK PENLSAKNLSDLFTIHSVADVQTLRFWNSYLKAIEDGKSATTMEDILIFATGCSSVPPTGFKPSLSVECL HVDFPVADKYRNHLVLPATNTYEEFQENMDFTIRDTLRLEKEERSHILPRTLNVSSNEEMLI SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001167964 |
ORF Size | 1866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001167964.1, NP_001161436.1 |
RefSeq Size | 6352 bp |
RefSeq ORF | 1869 bp |
Locus ID | 217558 |
UniProt ID | Q5RJY2 |
Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219864 | G2e3 (untagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3, (10ug) |
CNY 7,980.00 |
|
MR223648 | G2e3 (Myc-DDK-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3 |
CNY 6,180.00 |
|
MR223648L3 | Lenti ORF clone of G2e3 (Myc-DDK-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3 |
CNY 8,080.00 |
|
MR223648L4 | Lenti ORF clone of G2e3 (mGFP-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3 |
CNY 8,080.00 |