Srd5a2 (NM_053188) Mouse Tagged ORF Clone
CAT#: MG223814
- TrueORF®
Srd5a2 (tGFP-tagged) - Mouse steroid 5 alpha-reductase 2 (Srd5a2), (10ug)
ORF Plasmid: DDK
"NM_053188" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5ART2; S5AR 2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223814 representing NM_053188
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATTGTGTGCCATCAGGTCCCGGTGCTGGCAGGTAGCGCCACATTGGCCACCATGGGGACCCTGA TCCTGTGCTTTGGGAAACCCGCCAGTTACGGGAAACACAGCGAGAGTGTGTCGTCGGGAGTTCCCCTCCT GCCCGCACGCATAGCCTGGTTCCTGCAGGAGCTGCCTTCCTTTGTGGTGTCGGTAGGGATGCTCGCTTGG CAGCCGCGCTCCCTCTTTGGACCTCCGGGGAATGTGCTGCTGGGTCTCTTCTCCGCACATTACTTCCACA GGACATTTATTTACTCATTGCTCACCAGAGGCAGGCCTTTATCAGCGGTGATCTTTCTGAAAGCCACTGC CTTCTGCATAGGGAACGGACTCCTGCAAGCCTATTACCTGGTTTATTGCGCGGAATACCCTGAGGAGTGG TACACAGACATGCGGTTTAGCGTCGGTGTCTTCTTCTTTATTCTGGGGATGGGGATCAACATCCACAGTG ACTGCATGCTGCGCCAGCTCAGGAAGCCCGGAGAGGTCATCTACAGGATCCCACAAGGTGGCTTGTTTAC GTATGTGTCTGGAGCCAATTTCCTGGGCGAGATTATTGAATGGATGGGCTACGCTTTGGCCACTTGGTCT GTTCCAGCCTTTGCTTTTGCATTTTTCACACTTTGTTTCCTGGGGATGCAAGCTTTTTACCACCACAGGT TCTACCTCAAGATGTTTAAGGATTACCCCAAATCTAGGAAAGCCCTCATTCCATTCATCTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223814 representing NM_053188
Red=Cloning site Green=Tags(s) MPIVCHQVPVLAGSATLATMGTLILCFGKPASYGKHSESVSSGVPLLPARIAWFLQELPSFVVSVGMLAW QPRSLFGPPGNVLLGLFSAHYFHRTFIYSLLTRGRPLSAVIFLKATAFCIGNGLLQAYYLVYCAEYPEEW YTDMRFSVGVFFFILGMGINIHSDCMLRQLRKPGEVIYRIPQGGLFTYVSGANFLGEIIEWMGYALATWS VPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053188 |
ORF Size | 762 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053188.3 |
RefSeq Size | 854 bp |
RefSeq ORF | 765 bp |
Locus ID | 94224 |
UniProt ID | Q99N99 |
Gene Summary | Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212010 | Srd5a2 (untagged) - Mouse steroid 5 alpha-reductase 2 (Srd5a2), (10ug) |
CNY 3,990.00 |
|
MR223814 | Srd5a2 (Myc-DDK-tagged) - Mouse steroid 5 alpha-reductase 2 (Srd5a2) |
CNY 2,400.00 |
|
MR223814L3 | Lenti ORF clone of Srd5a2 (Myc-DDK-tagged) - Mouse steroid 5 alpha-reductase 2 (Srd5a2) |
CNY 4,750.00 |
|
MR223814L4 | Lenti ORF clone of Srd5a2 (mGFP-tagged) - Mouse steroid 5 alpha-reductase 2 (Srd5a2) |
CNY 4,800.00 |