Alg12 (NM_001142357) Mouse Tagged ORF Clone
CAT#: MG224017
- TrueORF®
Alg12 (tGFP-tagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast alpha-16-mannosyltransferase) (Alg12) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001142357" in other vectors (3)
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CNY 5,320.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ECM39 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224017 representing NM_001142357
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCAAGAAGTCATCGGGCAAGCGGTCATGGCCGCTTCTGGGGCTGCTGGTCACTGTAGCCACCA TCCACCTGGTTATATGTCCCTACACCAAGGTAGAAGAGAGCTTCAACCTTCAGGCCACACATGACCTCCT GTACCACCAGCTGGACATAGACAAGTACGACCACCACGAGTTTCCTGGAGTTGTTCCTAGGACGTTCCTC GGGCCGCTGGTGATCGCAGCGTTCTCCAGCCCCGTGGTTTATGTGCTCTCGCTTTTAGAAGTATCCAAAT TTTATTCTCAGCTGATAGTCAGAGGAGTCCTTGGGCTTGGTGTGATTTCTGGACTCTGGACATTACAAAA GGAAGTGAGACAGCAGTTCGGAGCCACGGTGGCTGTCATGTTCTGCTGGATATCAGCCACACAGTTTCAT CTCATGTTCTACTGTACGAGGACACTCCCCAACGTGTTGGCCCTGGCTGTGGTCTTACCAGCCCTCACAG CCTGGCTGCAGCGGAGGTGGGCCCTGTTTGTCTGGCTCTCAGCCTTCGTCATCATTGGCTTCAGGGCTGA GCTGGCCATGCTGCTGGGCATTGCGCTGCTGCTGACCTTGTACCAAAGAAGACTGACGGTGGCCAGAGTG CTCCGACACGCCATCCCAGCAGGGCTTCTCTGTCTAGGGCTGACGGTTGCTGTGGACTCCTATTTCTGGC GATACCTTGTATGGCCGGAAGGAGTGGTGCTTTGGTACAACACTGTCCTGAACAAAAGTTCCAACTGGGG CACTTCCCCACTCCTGTGGTATTTCTACTCAGCCCTGCCCCGAGGCCTGGGCTGCAGCCTTCTCTTCATA CCCCTGGGTGCAGTGGACAGGAGGACCTATGCACTGGCCCTGCCAAGCTTGGGATTCGTGGCTCTATACT CACTCCTCCCACACAAGGAGCTGCGGTTCATCATCTATACCTTCCCTGTCCTCAACATCATGGCTGCCAG AGGCTGCACCTATATCCTGAATAAAAAGTCTTGGCCATACAAGGTGAGAGCTATGCTGGTGACAGGGCAT ATCTTGGTGAATGTGGCATATACAGCCACGTCCCTCTATGTGTCTCATTTCAACTACCCTGGTGGGGTTG CGATGCAGCAGCTACATGAGCTGGTACCACCTCAGACAGATGTCCTTCTTCACATTGATGTGGCTGCTGC CCAGACAGGAGTGTCACGGTTTCTACAGGTCAATGATGACTGGAGGTATGACAAGAGTGAAGATGTGGGA GCAGCGGCCATGCTGAACTACACCCACATCCTCATGGAGGCAGTGCCTGGGCACCCGGCCCTCTACAGGG ACACACATCGAGTCCTGGCCAGTATTGAGGGCACTACAGGTATAAGCCTGAATTTGATGAAACTGCCACC CTTTGATGTCAACCTGCAGACAAAGCTGGTACTTCTAGAGCGGCTGCTTAGGCCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224017 representing NM_001142357
Red=Cloning site Green=Tags(s) MAGKKSSGKRSWPLLGLLVTVATIHLVICPYTKVEESFNLQATHDLLYHQLDIDKYDHHEFPGVVPRTFL GPLVIAAFSSPVVYVLSLLEVSKFYSQLIVRGVLGLGVISGLWTLQKEVRQQFGATVAVMFCWISATQFH LMFYCTRTLPNVLALAVVLPALTAWLQRRWALFVWLSAFVIIGFRAELAMLLGIALLLTLYQRRLTVARV LRHAIPAGLLCLGLTVAVDSYFWRYLVWPEGVVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFI PLGAVDRRTYALALPSLGFVALYSLLPHKELRFIIYTFPVLNIMAARGCTYILNKKSWPYKVRAMLVTGH ILVNVAYTATSLYVSHFNYPGGVAMQQLHELVPPQTDVLLHIDVAAAQTGVSRFLQVNDDWRYDKSEDVG AAAMLNYTHILMEAVPGHPALYRDTHRVLASIEGTTGISLNLMKLPPFDVNLQTKLVLLERLLRPA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142357 |
ORF Size | 1458 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142357.1, NP_001135829.1 |
RefSeq Size | 2131 bp |
RefSeq ORF | 1461 bp |
Locus ID | 223774 |
UniProt ID | Q8VDB2 |
Gene Summary | Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224017 | Alg12 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (Alg12), transcript variant 1 |
CNY 4,850.00 |
|
MR224017L3 | Lenti ORF clone of Alg12 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (Alg12), transcript variant 1 |
CNY 6,750.00 |
|
MR224017L4 | Lenti ORF clone of Alg12 (mGFP-tagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (Alg12), transcript variant 1 |
CNY 6,750.00 |