Rtn4rl2 (NM_199223) Mouse Tagged ORF Clone

CAT#: MG224387

  • TrueORF®

Rtn4rl2 (tGFP-tagged) - Mouse reticulon 4 receptor-like 2 (Rtn4rl2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_199223" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Ngr2; Ngrh1; Ngrl3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG224387 representing NM_199223
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCCGGGCTCCGGCGCCTGCTGCAAGGTCCTGCCTCAGCCTGCCTACTGCTGACACTCCTGGCCC
TTCCTTCCGTGACCCCCAGCTGTCCTATGCTCTGCACCTGCTACTCCTCCCCGCCCACCGTGAGCTGCCA
GGCCAACAACTTCTCCTCAGTGCCGCTGTCCTTGCCACCCAGTACACAGAGACTCTTCTTGCAGAACAAC
CTCATCCGCTCACTGCGGCCAGGCACCTTTGGGCCCAACCTGCTCACCCTGTGGCTCTTCTCCAACAACC
TCTCCACCATCCACCCTGGCACCTTCCGCCACCTGCAGGCCCTAGAAGAACTGGACCTCGGTGACAACCG
GCACCTGCGCTCCCTGGAGCCCGACACCTTCCAGGGTCTGGAGAGGCTGCAGTCACTACACCTGTATCGT
TGCCAGCTCAGCAGCCTGCCTGGCAACATTTTCCGAGGCTTGGTCAGCCTACAGTACCTCTACCTCCAGG
AGAACAGCCTGCTCCATCTACAGGATGACTTGTTCGCGGACCTGGCCAACCTGAGCCACCTCTTCCTCCA
CGGGAACCGCCTGCGGCTGCTCACGGAGCACGTGTTCCGCGGCTTGGGCAGCCTGGACCGGCTGTTGCTG
CACGGGAACCGGCTGCAGGGCGTGCACCGCGCGGCTTTCCACGGCCTCAGCCGCCTCACCATCCTCTACC
TGTTCAACAACAGCCTGGCCTCGCTGCCGGGAGAGGCGCTGGCCGACCTGCCGGCGCTCGAGTTCCTGCG
GCTCAACGCCAACCCCTGGGCGTGCGACTGCCGCGCTCGGCCGCTCTGGGCTTGGTTCCAGCGCGCGCGG
GTGTCCAGCTCCGACGTGACCTGCGCCACCCCGCCCGAGCGCCAGGGCCGGGACCTGCGCGCGCTGCGCG
ACTCCGATTTCCAAGCGTGCCCGCCGCCCACGCCCACGCGGCCGGGCAGCCGCGCCCGCGGCAACAGCTC
TTCCAACCACCTGTACGGCGTGGCCGAGGCTGGCGCTCCCCCCGCAGACCCGTCCACGCTCTACCGAGAT
CTGCCCGCCGAGGACTCGCGGGGGCGCCAGGGCGGGGACGCGCCCACCGAGGACGACTACTGGGGGGGCT
ACGGCGGCGAGGATCAGCGGGGCGAGCAGACGTGTCCCGGGGCCGCGTGCCAGGCGCCCGCAGACTCGCG
TGGCCCCGCGCTCTCGGCCGGGCTGCGCACCCCTCTGCTCTGCCTCTTGCCCCTGGCGCTCCATCACCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG224387 representing NM_199223
Red=Cloning site Green=Tags(s)

MLPGLRRLLQGPASACLLLTLLALPSVTPSCPMLCTCYSSPPTVSCQANNFSSVPLSLPPSTQRLFLQNN
LIRSLRPGTFGPNLLTLWLFSNNLSTIHPGTFRHLQALEELDLGDNRHLRSLEPDTFQGLERLQSLHLYR
CQLSSLPGNIFRGLVSLQYLYLQENSLLHLQDDLFADLANLSHLFLHGNRLRLLTEHVFRGLGSLDRLLL
HGNRLQGVHRAAFHGLSRLTILYLFNNSLASLPGEALADLPALEFLRLNANPWACDCRARPLWAWFQRAR
VSSSDVTCATPPERQGRDLRALRDSDFQACPPPTPTRPGSRARGNSSSNHLYGVAEAGAPPADPSTLYRD
LPAEDSRGRQGGDAPTEDDYWGGYGGEDQRGEQTCPGAACQAPADSRGPALSAGLRTPLLCLLPLALHHL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_199223
ORF Size 1260 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_199223.1, NP_954693.1
RefSeq Size 1263 bp
RefSeq ORF 1263 bp
Locus ID 269295
UniProt ID Q7M6Z0
Gene Summary Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG (By similarity). Plays a functionally redundant role in postnatal brain development (PubMed:27339102). Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum (PubMed:27339102). Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system (PubMed:22406547). Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG (PubMed:26335717). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:22325200).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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