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Mtm1 (NM_001164193) Mouse Tagged ORF Clone
CAT＃: MG224660
- TrueORF®
Mtm1 (tGFP-tagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001164193" in other vectors (4)
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CNY 2,090.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AF073996; mKIAA4176; Mtm |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224660 representing NM_001164193
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTGCATCAGCATCTAAGTATAATTCACACTCCTTGGAGAATGAATCCATTAAGAAAGTGTCTC AAGATGGAGTCAGTCAGGATGTGAGTGAGACTGTCCCTCGGCTCCCAGGGGAGTTACTAATTACTGAAAA AGAAGTTATTTACATATGTCCTTTCAATGGCCCCATTAAGGGAAGAGTTTACATCACAAATTATCGTCTT TATTTAAGAAGTTTGGAAACGGATTCTGCTCTAATACTTGATGTTCCTCTGGGTGTGATATCAAGAATTG AAAAAATGGGAGGCGCGACAAGTAGAGGAGAAAATTCCTATGGTCTAGATATTACTTGTAAAGATTTGAG AAACCTGAGGTTTGCATTGAAGCAAGAAGGCCACAGCAGAAGAGATATGTTTGAGATCCTTGTAAAACAT GCCTTTCCTCTGGCACACAATCTGGCTAACCTGAAACTCATGATCCTGTTACCTCGACCTCCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224660 representing NM_001164193
Red=Cloning site Green=Tags(s) MASASASKYNSHSLENESIKKVSQDGVSQDVSETVPRLPGELLITEKEVIYICPFNGPIKGRVYITNYRL YLRSLETDSALILDVPLGVISRIEKMGGATSRGENSYGLDITCKDLRNLRFALKQEGHSRRDMFEILVKH AFPLAHNLANLKLMILLPRPPE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164193 |
| ORF Size | 486 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164193.1, NP_001157665.1 |
| RefSeq Size | 770 bp |
| RefSeq ORF | 489 bp |
| Locus ID | 17772 |
| Gene Summary | Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology (By similarity). Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning (PubMed:21135508). Required for skeletal muscle maintenance but not for myogenesis (PubMed:12391329). In skeletal muscles, stabilizes MTMR12 protein levels (PubMed:23818870).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208991 | Mtm1 (untagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1), transcript variant 5, (10ug) |
CNY 1,320.00 |
|
| MR224660 | Mtm1 (Myc-DDK-tagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1), transcript variant 5 |
CNY 1,900.00 |
|
| MR224660L3 | Lenti ORF clone of Mtm1 (Myc-DDK-tagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1), transcript variant 5 |
CNY 3,800.00 |
|
| MR224660L4 | Lenti ORF clone of Mtm1 (mGFP-tagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1), transcript variant 5 |
CNY 3,800.00 |
