United States
Germany
Japan
United Kingdom
China
Mink1 (NM_001045964) Mouse Tagged ORF Clone
CAT＃: MG224723
- TrueORF®
Mink1 (tGFP-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1) transcript variant 4, (10ug)
ORF Plasmid: DDK
"NM_001045964" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 14,730.00
货期*
7周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B55; Map4k6; MINK; Ysk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224723 representing NM_001045964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATCGACCGCTCGCGGAAGAAGCGGGGTGAGAAAGAGGAGACAGAGTATGAGTACAGCGGCAGTGA GGAGGAAGACGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACA CTGCGCAGAGAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGC AGCTGCAGCAACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCG CATAGAGGAGCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAG CTACAAGAGAAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGC AAGCAGAGCGGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAG ACAGCTGCAGCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAG CAGCAGCAGCAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTA ATCCTGCTGACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTC TCCCTTGGCGAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCC CCAGGACCTCTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCC TGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCA GCCGACTCGAAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACT GCCACACCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTA GCCCAAACCCTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTC TATCGCCACTGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGACCT CGCAGTAACTCCGCCTGGCAAATCTATCTGCAGAGGCGGGCAGAGCGGGGCACCCCCAAGCCTCCCGGGC CCCCAGCTCAGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCTGACCTCAGGAGGAGTGACCCTGGCTG GGAGCGCTCAGACAGTGTCCTCCCGGCCTCCCACGGCCACCTCCCTCAGGCTGGCTCCTTGGAGCGGAAC CGAAACCGTGTGGGAGCCTCCACAAAACTGGATAGCTCTCCAGTGCTCTCCCCTGGGAACAAAGCCAAGC CTGAAGACCACCGCTCAAGGCCAGGCCGGCCCGCAAGCTATAAGCGAGCAATTGGCGAGGACTTTGTGTT GCTCAAAGAGCGGACTCTGGATGAGGCCCCTAAGCCTCCCAAGAAGGCCATGGACTACTCCTCATCCAGT GAGGAGGTGGAAAGCAGTGAAGAGGAGGAGGAGGAAGGCGATGGGGAGCCGTCAGAGGGGAGCAGAGACA CTCCCGGGGGCCGTGATGGTGATACAGACAGCGTCAGCACCATGGTGGTTCATGATGTTGAGGAGATATC CGGGACCCAGCCCTCATATGGCGGCGGCACCATGGTGGTCCAGCGTACTCCTGAAGAGGAACGAAGCCTG CTGCTTGCTGATAGCAATGGCTACACAAACCTGCCTGATGTGGTCCAGCCCAGCCACTCACCTACTGAGA ACAGCAAAGGTCAAAGCCCTCCAACAAAGGATGGAGGCAGTGATTACCAGTCTCGTGGGCTGGTAAAGGC CCCAGGAAAGAGCTCATTCACCATGTTTGTGGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACACC ATCCCTATCACAGCCCTAGTGGGTGGAGAAGGTGGTCGCCTTGATCAACTGCAGTTCGATGTGAGGAAGG GCTCTGTGGTCAACGTCAATCCCACCAACACCCGAGCTCATAGTGAAACTCCTGAAATTCGCAAGTACAA GAAGCGATTCAACTCAGAGATCCTATGTGCAGCTCTCTGGGGGGTCAACCTCCTAGTGGGCACAGAGAAT GGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAGGTGTATGGACTTATTGGGCGACGACGCTTCCAGC AAATGGATGTCTTAGAAGGGCTCAACTTGCTCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTATA TTACCTGTCCTGGCTTCGGAACAAGATCCTACACAATGACCCAGAGGTGGAAAAGAAGCAGGGGTGGACC ACCGTGGGGGACATGGAGGGCTGCGGCCACTACCGTGTTGTGAAATATGAACGGATTAAGTTCCTGGTCA TTGCCCTGAAGAACTCCGTGGAGGTTTATGCCTGGGCTCCCAAACCCTACCACAAATTCATGGCCTTCAA GTCCTTTGCTGACCTCCCTCACCGCCCTCTACTGGTGGACCTGACAGTAGAGGAGGGACAGCGGCTCAAG GTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATGTTGATTCTGGGAACAGCTATGACATCTACA TCCCTGTACATATCCAGAGCCAGATCACACCCCACGCCATCATCTTCCTCCCCAACACTGATGGCATGGA GATGCTGCTGTGCTATGAAGATGAGGGTGTCTATGTCAACACTTACGGGCGGATCATCAAGGATGTGGTG CTGCAGTGGGGAGAGATGCCCACCTCTGTGGCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGA AGGCCATAGAGATCCGCTCTGTGGAGACAGGCCACCTAGATGGGGTCTTCATGCACAAACGAGCCCAGAG GCTCAAGTTCCTGTGTGAGCGCAATGACAAGGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAAGCAGCCAA GTTTACTTTATGACTCTGAACCGTAACTGCATCATGAACTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224723 representing NM_001045964
Red=Cloning site Green=Tags(s) MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRG LAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPT EQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGEST LRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRK LQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQK QQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSP PGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPT ATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARP RSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERN RNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSS EEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSL LLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDT IPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTEN GLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWT TVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLK VIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVV LQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQ VYFMTLNRNCIMNW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001045964 |
| ORF Size | 4032 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001045964.1, NP_001039429.1 |
| RefSeq Size | 4994 bp |
| RefSeq ORF | 4035 bp |
| Locus ID | 50932 |
| Gene Summary | Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
