Mccc2 (NM_030026) Mouse Tagged ORF Clone
CAT#: MG224804
- TrueORF®
Mccc2 (tGFP-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_030026" in other vectors (4)
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CNY 5,510.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4930552N12Rik; MCCB |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224804 representing NM_030026
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGCGCGCTGAGGTCAGCTCTGCGACCGTGCTGTCGGGCTGCTGTACCCCCGCAGCGTGCCTATC ACGGGGACTCAGTGGCCCGACTGGGCACCCAGCCTGACTCGGCCTCCTCCACCTACCAGGAGAACTATGA GCAGATGAAAGCTTTAGTGAGTCAACTTCATGAGCGAGCCCAGTATGTGAGACTAGGAGGGAGTGAGAAG GCCCGGGCGCGGCACACATCCAGAGGAAAACTGCTCCCCAGAGACAGAATCGACAATCTCATAGACCCAG GGTCTCCCTTTCTGGAATTCTCCCAGTTTGCAGGTTACCAGTTATATGGCGACGAGGAAGTCCCCGCCGG TGGCATCATCACGGGCATTGGGCGAGTGTCGGGGGTAGAATGCATGATTGTGGCTAACGATGCCACCGTC AAAGGAGGCACCTACTACCCAGTGACTGTGAAAAAGCACGTGCGGGCACAAGAGATTGCGCTGCAGAACC GACTCCCATGCATCTATCTGGTTGACTCAGGAGGGGCCAACTTACCTCGGCAAGCAGACACCTTTCCAGA CCGGGACCACTTTGGCCGCATATTCTATAATCAGGCAATTATGTCTTCTAAAAACATCACACAGATAGCT GTGGTCATGGGCTCCTGTACGGCAGGAGGTGCGTATGTGCCTGCAATGGCTGATGAAAACATCATTGTAC AGAAGCAGGGTACCATCTTCTTGGCTGGACCACCCTTGGTGAAGGCAGCTACAGGTGAAGAGGTGTCCGC TGAGGATCTCGGAGGTGCCGACCTGCATTGCAGAAAGTCTGGGGTCACTGACCACTATGCTTTGGACGAC CACCACGCCCTCCACTTAACAAGGAAGGTTGTGAGGAGTCTCAATTATCAGAAGAAAATGGATGTTACCA TTGAGCCTTCTGAGGAGCCCTTGTTCCCTGCAGATGAGCTCTATGGAATAGTTGGTGCAAACCTGAAGAG GAGCTTTGATGTCCGAGAGGTCATTGCTAGAATCGTGGATGGAAGCAGATTCAATGAGTTCAAGGCCTTA TATGGAGACACCCTAGTTACAGGATTTGCTCGAATATTTGGGTACCCCGTTGGCATCATTGGAAACAATG GAGTTTTGTTTTCTGAATCTGCTAAGAAGGGTGCTCACTTCGTCCAGCTGTGCTGCCAGAGAAATATCCC TCTGCTGTTTCTTCAGAATATCACCGGATTCATGGTTGGCAGAGACTATGAAGCTGAAGGGATTGCCAAG GATGGTGCCAAAATGGTAGCTGCAGTAGCCTGTGCCAAAGTGCCTAAGATAACTGTCATAATTGGGGGAT CGTACGGGGCTGGAAACTACGGAATGTGTGGCAGAGCATACAGTCCAAGGTTTCTCTACATGTGGCCAAA TGCCCGCATCTCTGTGATGGGAGGAGAGCAGGCAGCCACGGTGTTAGCCACAGTGGCTAGAGACCAGAAA GCACGCGAGGGGAAGCAGTTCTCCAGTGCAGAGGAGGCAGCTTTGAAAGAACCCATCATTAAGAGGTTCG AAGAGGAAGGGAACCCTTACTACTCTAGTGCAAGGCTGTGGGACGATGGGATTATTGACCCGGTGGACAC CAGGCTGGTCCTGGGGCTCAGCCTCAGTGCAGCCCTCAATGCGCCCATCCAGAGGACTGACTTTGGCATC TTCAGGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224804 representing NM_030026
Red=Cloning site Green=Tags(s) MWGALRSALRPCCRAAVPPQRAYHGDSVARLGTQPDSASSTYQENYEQMKALVSQLHERAQYVRLGGSEK ARARHTSRGKLLPRDRIDNLIDPGSPFLEFSQFAGYQLYGDEEVPAGGIITGIGRVSGVECMIVANDATV KGGTYYPVTVKKHVRAQEIALQNRLPCIYLVDSGGANLPRQADTFPDRDHFGRIFYNQAIMSSKNITQIA VVMGSCTAGGAYVPAMADENIIVQKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVTDHYALDD HHALHLTRKVVRSLNYQKKMDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFNEFKAL YGDTLVTGFARIFGYPVGIIGNNGVLFSESAKKGAHFVQLCCQRNIPLLFLQNITGFMVGRDYEAEGIAK DGAKMVAAVACAKVPKITVIIGGSYGAGNYGMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQK AREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSLSAALNAPIQRTDFGI FRM TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030026 |
ORF Size | 1689 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030026.2, NP_084302.1 |
RefSeq Size | 2146 bp |
RefSeq ORF | 1692 bp |
Locus ID | 78038 |
UniProt ID | Q3ULD5 |
Gene Summary | Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219103 | Mccc2 (untagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,220.00 |
|
MR224804 | Mccc2 (Myc-DDK-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein |
CNY 4,200.00 |
|
MR224804L3 | Lenti ORF clone of Mccc2 (Myc-DDK-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein |
CNY 6,940.00 |
|
MR224804L4 | Lenti ORF clone of Mccc2 (mGFP-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein |
CNY 6,940.00 |