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Tnr (NM_022312) Mouse Tagged ORF Clone
CAT＃: MG225166
- TrueORF®
Tnr (tGFP-tagged) - Mouse tenascin R (Tnr), (10ug)
ORF Plasmid: DDK
"NM_022312" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | J1-tenascin; janusin; restrictin; TN-R |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225166 representing NM_022312
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCGATGGGGAAACAGTAGTCTTGAAGAACATGCTCATTGGTGTAAACCTGATCCTGTTGGGAT CCATGCTCAAGCCTTCTGAGTGTCGGCTGGAGGTGACTACAGAAAGGGCTCAGAGACAAACTGTGGAGGA GGAAGGAGGGGCTTCCAGCTACAATACTTCCAGCAAGGAACAGCCTATGGTCTTCAACCATGTGTATAAC ATCAACGTGCCACTTGAAAGCCTCTGCTCCTCGGGGCTGGAGGCCTCAGCTGAGCAGGACATGAGTGCTG AAGATGATACTCTGGCCGAATACATAGGCCAAACCTCAGACCATGAAAGCCAGGTTACCTTCACCCACAA GATCAACCTCCCCAAAAAAGCCTGCCCATGTGCAAGCTCTTCCCAGGTACTGCAGGAACTGCTGAGCCGG ATCGAGATGCTGGAGAGGGAGGTGTCCTTGCTGCGAGACCAGTGCAATACCAACTGCTGTCAGGAAAGCG CTGCCACAGGACAACTGGACTATGTCCCTCACTGCAGCGGCCATGGCAACTTTAGCTTCGAGTCCTGTGG CTGCATCTGCAATGAAGGCTGGTTTGGCAAGAACTGCTCAGAGCCCTACTGCCCACTGGGCTGCTCCAGT CGGGGTGTGTGTGTCGATGGCCAGTGCATTTGTGACAGTGAATACAGCGGAGATGACTGTTCAGAGCTCC GGTGCCCAACAGACTGCAGTTCCCGAGGGCTCTGTGTGGATGGGGAATGTGTCTGTGAAGAGCCCTACAC AGGCGAGGACTGCAGGGAGCTGCGCTGCCCTGGGGACTGTTCAGGGAAGGGGCAATGTGCCAATGGTACC TGCCTGTGCCAAGAGGGCTATGCTGGTGAGGACTGCAGCCAGCGACGGTGTCTGAATGCCTGCAGTGGGC GAGGTCACTGCCAGGAGGGGCTCTGCATCTGTGAGGAAGGCTACCAGGGCCCTGACTGCTCAGCAGTTGC CCCTCCAGAGGACTTGCGAGTGGCTGGTATCAGCGACAGGTCCATTGAGCTGGAATGGGACGGGCCGATG GCAGTGACGGAATATGTGATCTCTTACCAGCCGACGGCCCTGGGGGGCCTTCAGCTCCAGCAGCGGGTGC CTGGAGATTGGAGTGGTGTCACCATCATGGAGCTGGAGCCAGGTCTCACCTACAACATCAGCGTCTACGC TGTCATTAGCAACATCCTCAGCCTTCCCATCACTGCCAAGGTGGCCACTCATCTCTCCACTCCTCAAGGG CTACAGTTCAAGACGATCACAGAGACCACCGTGGAGGTGCAGTGGGAGCCCTTCTCTTTCTCCTTCGACG GGTGGGAGATCAGCTTCATTCCAAAGAACAATGAAGGAGGGGTGATTGCTCAGCTCCCCAGCGATGTGAC GTCCTTTAACCAGACAGGACTGAAACCTGGGGAGGAGTACATTGTGAATGTTGTGGCTCTAAAGGAACAA GCCCGGAGCCCTCCTACCTCTGCCAGCGTCTCCACTGTCATTGACGGGCCCACACAGATCCTGGTTCGAG ATGTCTCTGATACTGTGGCTTTTGTGGAATGGACCCCACCTCGAGCCAAAGTTGATTTCATTCTTTTAAA ATATGGCTTGGTGGGTGGCGAAGGCGGGAAGACGACCTTCCGGCTGCAGCCTCCCTTAAGCCAGTACTCC GTGCAAGCCCTTAGACCTGGCTCGCGCTACGAGGTGTCCATCAGCGCAGTCCGGGGGACCAATGAGAGTG AAGCCTCAAGCACCCAATTTACAACAGAGATTGATGCTCCCAAGAATTTGCGAGTGGGTTCCCGCACAGC AACTAGCCTTGACCTGGAATGGGATAACAGCGAGGCCGAAGCTCAGGAGTACAAGGTTGTGTACAGCACC TTAGCTGGTGAACAGTACCACGAAGTGCTGGTACCCAAGGGCATTGGCCCAACTACCAAGACTACCCTCA CAGATCTGGTTCCAGGCACAGAGTATGGAGTTGGAATATCTGCAGTAATGAACTCAAAACAAAGCATTCC TGCCACTATGAATGCCAGGACTGAACTTGACAGTCCCCGAGACCTCATGGTAACAGCCTCCTCAGAGACC TCTATCTCTCTCATCTGGACCAAGGCCAGTGGTCCCATTGATCACTACAGAATTACTTTTACCCCATCTT CTGGGATCTCCTCCGAAGTCACTGTGCCCAGGGATAGGACTTCATACACACTAACAGATCTAGAGCCTGG AGCAGAATACATTATCTCCATCACTGCTGAGAGGGGTCGGCAGCAGAGCCTGGAGTCTACTGTGGATGCC TTCACAGGCTTCCGCCCTATCTCCCATTTGCACTTTTCTCATGTGACCTCCTCCAGTGTCAATATCACCT GGAGTGACCCATCTCCCCCAGCAGACAGACTCATTCTGAACTACAGCCCCAGGGACAAAGAGGAAGACAT GTTGGAGGTCCTCTTGGATGCCACCAAGAGGCACGCTGTCTTGATGGGTCTACAGCCAGCCACTGAATAT ATAGTGAACCTTGTAGCTGTCCATGGGACGGTAACCTCTGAACCCATAGTGGGTTCTATCACTACAGGAA TTGATCCTCCCAAAAACATCACAATTAGCAACGTGACTAAGGACTCCCTGACGGTGTCCTGGAGCTCTCC TGTTGCGCCTTTTGATTACTACCGAGTATCGTACCGACCCACCCAAGTGGGACGGCTAGACAGCTCCGTA GTGCCCAACACCGTGACAGAGTTCGCCATCACCAGGCTGTATCCAGCTACTGAATATGAAATAAGCCTCA ACAGTGTACGGGGCAGGGAGGAGAGTGAACGCATCTGCACCCTGGTGCACACAGCCATGGATAGCCCCAT GGATTTGATCGCTACCAACATCACACCTACAGAAGCCTTGCTCCAGTGGAAAGCACCCATGGGTGAAGTG GAAAATTATGTCATCGTCCTCACACACTTTGCAATTGCTGGAGAGACCATCCTGGTTGACGGGGTCAGCG AAGAATTCCAGCTTGTAGACTTGCTTCCTAGCACCCACTACACTGTCACTATGTATGCCACCAGTGGGCC TCTCATGAGTGGCACCATTGCCACCAACTTCTCCACCCTCCTGGACCCTCCTGACAACCTGACAGCCAGT GAAGTCACCAGGCAAAGCGCACTGATCTCCTGGCAGCCGCCCAGAGCTGCGATTGAAAACTATGTCTTGA CATACAAGTCCACCGATGGAAGCCGCAAAGAGCTGATAGTGGATGCTGAGGACACCTGGATCCGACTAGA GGGCCTGTCAGAGAACACAGACTACACAGTGCTCCTGCAGGCAGCCCAGGAGGCCACAAGGAGCAGTCTC ACCTCTACTGTCTTTACCACAGGGGGCCGGGTGTTCTCTCATCCTCAAGACTGTGCCCAGCATTTGATGA ATGGAGACACTCTGAGTGGCGTTTACACCATCTTCCTCAATGGGGAGCTAAGCCACAAGCTGCAAGTGTA CTGTGACATGACCACAGATGGGGGCGGCTGGATTGTATTCCAGAGGCGGCAAAATGGACAAACTGATTTT TTCCGGAAATGGGCAGATTATCGTGTTGGCTTTGGGAATCTGGAGGATGAGTTTTGGCTAGGGCTAGACA ACATCCACAGGATAACAGCCCAGGGCCGCTATGAGCTGCGTGTGGATATGCGGGATGGACAGGAAGCAGT CTTTGCCTACTATGACAAGTTCGCTGTGGAGGACAGCAGAAGCCTGTACAAGATCCGCATAGGAAGTTAC AATGGCACTGCAGGAGACTCCCTCAGCTATCACCAGGGACGTCCTTTCTCCACTGAGGACAGAGACAATG ATGTTGCAGTCACCAACTGTGCCATGTCCTACAAGGGTGCTTGGTGGTATAAGAACTGCCACCGGACCAA CCTCAACGGGAAGTACGGGGAGTCCAGGCACAGTCAGGGGATCAACTGGTACCATTGGAAAGGCCATGAA TTCTCCATCCCCTTTGTAGAAATGAAGATGAGGCCCTACATCCATCGTCTCACAGCCGGGAGGAAACGGC GAGCCTTGAAATTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225166 representing NM_022312
Red=Cloning site Green=Tags(s) MGIDGETVVLKNMLIGVNLILLGSMLKPSECRLEVTTERAQRQTVEEEGGASSYNTSSKEQPMVFNHVYN INVPLESLCSSGLEASAEQDMSAEDDTLAEYIGQTSDHESQVTFTHKINLPKKACPCASSSQVLQELLSR IEMLEREVSLLRDQCNTNCCQESAATGQLDYVPHCSGHGNFSFESCGCICNEGWFGKNCSEPYCPLGCSS RGVCVDGQCICDSEYSGDDCSELRCPTDCSSRGLCVDGECVCEEPYTGEDCRELRCPGDCSGKGQCANGT CLCQEGYAGEDCSQRRCLNACSGRGHCQEGLCICEEGYQGPDCSAVAPPEDLRVAGISDRSIELEWDGPM AVTEYVISYQPTALGGLQLQQRVPGDWSGVTIMELEPGLTYNISVYAVISNILSLPITAKVATHLSTPQG LQFKTITETTVEVQWEPFSFSFDGWEISFIPKNNEGGVIAQLPSDVTSFNQTGLKPGEEYIVNVVALKEQ ARSPPTSASVSTVIDGPTQILVRDVSDTVAFVEWTPPRAKVDFILLKYGLVGGEGGKTTFRLQPPLSQYS VQALRPGSRYEVSISAVRGTNESEASSTQFTTEIDAPKNLRVGSRTATSLDLEWDNSEAEAQEYKVVYST LAGEQYHEVLVPKGIGPTTKTTLTDLVPGTEYGVGISAVMNSKQSIPATMNARTELDSPRDLMVTASSET SISLIWTKASGPIDHYRITFTPSSGISSEVTVPRDRTSYTLTDLEPGAEYIISITAERGRQQSLESTVDA FTGFRPISHLHFSHVTSSSVNITWSDPSPPADRLILNYSPRDKEEDMLEVLLDATKRHAVLMGLQPATEY IVNLVAVHGTVTSEPIVGSITTGIDPPKNITISNVTKDSLTVSWSSPVAPFDYYRVSYRPTQVGRLDSSV VPNTVTEFAITRLYPATEYEISLNSVRGREESERICTLVHTAMDSPMDLIATNITPTEALLQWKAPMGEV ENYVIVLTHFAIAGETILVDGVSEEFQLVDLLPSTHYTVTMYATSGPLMSGTIATNFSTLLDPPDNLTAS EVTRQSALISWQPPRAAIENYVLTYKSTDGSRKELIVDAEDTWIRLEGLSENTDYTVLLQAAQEATRSSL TSTVFTTGGRVFSHPQDCAQHLMNGDTLSGVYTIFLNGELSHKLQVYCDMTTDGGGWIVFQRRQNGQTDF FRKWADYRVGFGNLEDEFWLGLDNIHRITAQGRYELRVDMRDGQEAVFAYYDKFAVEDSRSLYKIRIGSY NGTAGDSLSYHQGRPFSTEDRDNDVAVTNCAMSYKGAWWYKNCHRTNLNGKYGESRHSQGINWYHWKGHE FSIPFVEMKMRPYIHRLTAGRKRRALKF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022312 |
| ORF Size | 4074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022312.3, NP_071707.2 |
| RefSeq Size | 5395 bp |
| RefSeq ORF | 4077 bp |
| Locus ID | 21960 |
| UniProt ID | Q8BYI9 |
| Gene Summary | Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Theses interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediates inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224323 | Tnr (untagged) - Mouse tenascin R (Tnr), (10ug) |
CNY 17,390.00 |
|
| MR225166 | Tnr (Myc-DDK-tagged) - Mouse tenascin R (Tnr) |
CNY 9,296.00 |
|
| MR225166L3 | Lenti ORF clone of Tnr (Myc-DDK-tagged) - Mouse tenascin R (Tnr) |
CNY 12,730.00 |
|
| MR225166L4 | Lenti ORF clone of Tnr (mGFP-tagged) - Mouse tenascin R (Tnr) |
CNY 12,730.00 |
