Hipk1 (NM_010432) Mouse Tagged ORF Clone
CAT#: MG225509
- TrueORF®
Hipk1 (tGFP-tagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1), (10ug)
ORF Plasmid: DDK
"NM_010432" in other vectors (4)
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CNY 10,550.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110062K04Rik; Myak |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225509 representing NM_010432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCACAGCTGCAGGTGTTTTCGCCCCCATCAGTGTCGTCGAGTGCCTTCTGCAGTGCAAAGAAAC TGAAAATAGAGCCCTCTGGCTGGGATGTTTCAGGACAGAGCAGCAACGACAAATACTATACCCACAGCAA AACCCTCCCAGCTACACAAGGGCAAGCCAGCTCCTCTCACCAGGTAGCAAATTTCAATCTTCCTGCTTAC GACCAGGGCCTCCTTCTCCCAGCTCCTGCCGTGGAGCATATTGTGGTAACAGCTGCTGATAGCTCAGGCA GCGCCGCTACAGCAACCTTCCAAAGCAGCCAGACCCTGACTCACAGGAGCAACGTTTCTTTGCTTGAGCC ATATCAAAAATGTGGATTGAAGAGAAAGAGTGAGGAAGTGGAGAGCAACGGTAGCGTGCAGATCATAGAA GAACACCCCCCTCTCATGCTGCAGAACAGAACCGTGGTGGGTGCTGCTGCCACGACCACCACTGTGACCA CCAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGAC CAACAGCTATGAAGTCCTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCAAAGTGCTGGAAGCGG AGCACCAAAGAAATTGTGGCCATTAAGATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTG AAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAACTTTGTCCGTTCTTATGAGTG TTTTCAGCACAAGAATCATACCTGCCTTGTGTTTGAGATGTTGGAGCAGAACTTGTACGATTTTCTAAAG CAGAACAAGTTTAGCCCACTGCCACTCAAGTACATAAGACCAATCTTGCAGCAGGTGGCCACAGCCCTGA TGAAGCTGAAGAGTCTTGGTCTGATTCATGCTGACCTTAAACCTGAAAACATAATGCTAGTCGATCCAGT TCGCCAACCCTACCGAGTGAAGGTCATTGACTTTGGTTCTGCTAGTCATGTTTCCAAAGCCGTGTGTTCA ACCTACCTGCAATCACGCTACTACAGAGCTCCTGAAATTATCCTTGGATTACCATTCTGTGAAGCTATTG ACATGTGGTCACTGGGCTGTGTAATAGCTGAGCTGTTCCTGGGATGGCCTCTTTATCCTGGTGCTTCAGA ATACGATCAGATTCGCTATATTTCACAAACACAAGGCCTGCCAGCTGAGTATCTTCTCAGTGCCGGAACA AAAACAACCAGGTTTTTTAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCTGAAG AACATGAATTGGAAACTGGAATAAAGTCAAAAGAAGCTCGGAAGTACATTTTTAACTGTTTAGATGACAT GGCTCAGGTAAATATGTCTACAGACTTAGAGGGGACAGATATGTTAGCAGAGAAAGCAGATCGGAGAGAG TATATTGATCTTCTAAAGAAAATGCTGACGATTGATGCAGATAAGAGAATCACGCCTCTGAAGACTCTTA ACCACCAATTTGTGACGATGAGTCACCTCCTGGACTTTCCTCACAGCAGCCACGTTAAGTCCTGTTTCCA GAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGACACAGTGAGTCAGATCAAGAGTCCCTTCACT ACACATGTCGCTCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAACCAGCTCAACACAGTGCACA ATCAGGCCAGTGTTCTAGCTTCCAGCTCTACTGCAGCAGCAGCTACCCTTTCTCTGGCTAATTCAGATGT CTCGCTGCTAAACTACCAATCGGCTTTGTACCCATCGTCGGCAGCGCCAGTTCCTGGAGTTGCCCAGCAG GGTGTTTCCTTACAACCTGGAACCACCCAGATCTGCACTCAGACAGATCCATTCCAGCAAACATTTATAG TATGCCCACCTGCTTTTCAGACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGA TAATGCTGTGCCAATTGTACCCCAGGCGCCTGCTGCTCAGCCGCTGCAGATCCAGTCAGGAGTACTCACA CAGGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACGCCGCAGTATG CGGTGCCCTTTACCCTGAGCTGCGCAGCAGGCCGGCCGGCGCTGGTTGAACAGACTGCTGCTGTACTGCA AGCCTGGCCTGGAGGAACCCAACAAATTCTCCTGCCTTCAGCCTGGCAGCAGCTGCCCGGGGTAGCTCTG CACAACTCTGTCCAGCCTGCTGCAGTGATTCCAGAGGCCATGGGGAGCAGCCAACAGCTAGCTGACTGGA GGAATGCCCACTCTCATGGCAACCAGTACAGCACTATTATGCAGCAGCCATCTTTGCTGACCAACCATGT GACCTTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAGTCTAGTTCC CTCCCTTCAAAGAAGAATAAGCAGTCTGCTCCAGTTTCATCCAAATCCTCTCTGGAAGTCCTGCCTTCTC AAGTTTATTCTCTGGTTGGGAGTAGTCCTCTTCGTACCACATCTTCTTATAATTCCCTAGTTCCTGTCCA AGACCAGCATCAGCCAATCATCATTCCAGATACCCCCAGCCCTCCTGTGAGTGTCATCACTATCCGTAGT GACACTGATGAAGAAGAGGACAACAAATACAAGCCCAATAGCTCGAGCCTGAAGGCGAGGTCTAATGTCA TCAGTTATGTCACTGTCAATGATTCTCCAGACTCTGACTCCTCCCTGAGCAGCCCACATCCCACAGACAC TCTGAGTGCTCTGCGGGGCAACAGTGGGACCCTTCTGGAGGGACCTGGCAGACCTGCAGCAGATGGCATT GGCACCCGTACTATCATTGTGCCTCCTTTGAAAACACAGCTTGGCGACTGCACTGTAGCAACACAGGCCT CAGGTCTCCTTAGCAGTAAGACCAAGCCAGTGGCCTCAGTGAGTGGGCAGTCATCTGGATGCTGTATCAC TCCCACGGGGTACCGGGCTCAGCGAGGGGGAGCCAGCGCGGTGCAGCCACTCAACCTTAGCCAGAACCAG CAGTCATCGTCAGCTTCAACCTCGCAGGAAAGAAGCAGCAACCCTGCTCCCCGCAGACAGCAGGCATTTG TGGCCCCGCTCTCCCAAGCCCCCTACGCCTTCCAGCATGGCAGCCCACTGCACTCGACGGGGCACCCACA CTTGGCCCCAGCCCCTGCTCACCTGCCAAGCCAGCCTCACCTGTATACGTACGCTGCCCCCACTTCTGCT GCTGCATTGGGCTCCACCAGTTCCATTGCTCATCTGTTCTCCCCCCAGGGTTCCTCAAGGCATGCTGCAG CTTATACCACACACCCTAGCACTCTGGTGCATCAGGTTCCTGTCAGTGTCGGGCCCAGCCTCCTCACTTC TGCCAGTGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACTCAGTCCTACATCGGGTCTTCCCGAGGC TCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGTCAGTATTCTTACTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225509 representing NM_010432
Red=Cloning site Green=Tags(s) MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVANFNLPAY DQGLLLPAPAVEHIVVTAADSSGSAATATFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVESNGSVQIIE EHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKR STKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLK QNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCS TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGT KTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRRE YIDLLKKMLTIDADKRITPLKTLNHQFVTMSHLLDFPHSSHVKSCFQNMEICKRRVHMYDTVSQIKSPFT THVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQ GVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLT QGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSAWQQLPGVAL HNSVQPAAVIPEAMGSSQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSS LPSKKNKQSAPVSSKSSLEVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRS DTDEEEDNKYKPNSSSLKARSNVISYVTVNDSPDSDSSLSSPHPTDTLSALRGNSGTLLEGPGRPAADGI GTRTIIVPPLKTQLGDCTVATQASGLLSSKTKPVASVSGQSSGCCITPTGYRAQRGGASAVQPLNLSQNQ QSSSASTSQERSSNPAPRRQQAFVAPLSQAPYAFQHGSPLHSTGHPHLAPAPAHLPSQPHLYTYAAPTSA AALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRG STIYTGYPLSPTKISQYSYL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010432 |
ORF Size | 3630 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010432.2, NP_034562.2 |
RefSeq Size | 8065 bp |
RefSeq ORF | 3633 bp |
Locus ID | 15257 |
UniProt ID | O88904 |
Gene Summary | Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223939 | Hipk1 (untagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1), (10ug) |
CNY 15,490.00 |
|
MR225509 | Hipk1 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1) |
CNY 8,432.00 |
|
MR225509L3 | Lenti ORF clone of Hipk1 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1) |
CNY 11,500.00 |
|
MR225509L4 | Lenti ORF clone of Hipk1 (mGFP-tagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1) |
CNY 11,500.00 |