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Rtn4 (NM_194053) Mouse Tagged ORF Clone
CAT＃: MG226122
- TrueORF®
Rtn4 (tGFP-tagged) - Mouse reticulon 4 (Rtn4) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_194053" in other vectors (4)
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CNY 4,090.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226122 representing NM_194053
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACATAGACCAGTCGTCGCTGGTCTCCTCGTCCGCGGATAGCCCGCCCCGGCCCCCGCCCGCTT TCAAGTACCAGTTCGTGACGGAGCCCGAGGACGAGGAGGACGAGGAAGACGAGGAGGAGGAGGAGGACGA CGAGGACCTGGAGGAATTGGAGGTGCTGGAGAGGAAGCCCGCAGCCGGGCTGTCCGCGGCTCCGGTGCCC CCCGCCGCCGCACCGCTGCTGGACTTCAGCAGCGACTCGGTGCCCCCCGCGCCCCGCGGGCCGCTGCCGG CCGCGCCCCCCACCGCCCCTGAGAGGCAGCCGTCCTGGGAACGCAGCCCCGCGGCGTCCGCGCCATCCCT GCCGCCCGCTGCCGCAGTCCTGCCCTCCAAGCTCCCGGAGGACGACGAGCCTCCAGCGCGGCCTCCGGCG CCAGCCGGCGCGAGCCCCCTAGCGGAGCCCGCCGCGCCCCCTTCCACGCCGGCCGCGCCCAAGCGCAGGG GCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCTGC AGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTG CTGCTGTCTCTGACAGTGTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGA CTATCAGCTTTAGGATATATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAG GGCATATTTGGAATCTGAAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGT CATGTGAACAGCACAATAAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGT TTGCAGTGTTGATGTGGGTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGC TCTGATCTCACTCTTCAGTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGA CTTGCAAACAAGAGCGTTAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAG CAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226122 representing NM_194053
Red=Cloning site Green=Tags(s) MEDIDQSSLVSSSADSPPRPPPAFKYQFVTEPEDEEDEEDEEEEEDDEDLEELEVLERKPAAGLSAAPVP PAAAPLLDFSSDSVPPAPRGPLPAAPPTAPERQPSWERSPAASAPSLPPAAAVLPSKLPEDDEPPARPPA PAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAVVDLLYWRDIKKTGVVFGASLFL LLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALG HVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLG LANKSVKDAMAKIQAKIPGLKRKAE TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194053 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194053.3, NP_918942.1 |
| RefSeq Size | 3804 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 68585 |
| UniProt ID | Q99P72 |
| Gene Summary | Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210753 | Rtn4 (untagged) - Mouse reticulon 4 (Rtn4), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
| MR226122 | Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 3 |
CNY 3,710.00 |
|
| MR226122L3 | Lenti ORF clone of Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 3 |
CNY 5,610.00 |
|
| MR226122L4 | Lenti ORF clone of Rtn4 (mGFP-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 3 |
CNY 5,610.00 |
