Pcyt1a (NM_001163159) Mouse Tagged ORF Clone
CAT#: MG226245
- TrueORF®
Pcyt1a (tGFP-tagged) - Mouse phosphate cytidylyltransferase 1 choline alpha isoform (Pcyt1a) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001163159" in other vectors (3)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cctalpha; CTalpha; Ctpct; Cttalpha |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226245 representing NM_001163159
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCACAGAGTTCAGCTAAAGTCAATTCGAGGAAGAGGAGGAAAGAGGCACCTGGCCCTAATGGAG CAACAGAGGAAGATGGAATTCCTTCCAAAGTGCAGCGTTGCGCAGTTGGTTTACGGCAGCCAGCTCCTTT TTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTCAGGGTGACTATGGAAGAAGCCTGCAGGGGA ACTCCGTGTGAGCGGCCTGTGAGAGTTTATGCGGATGGAATATTTGACTTGTTTCACTCTGGTCATGCCC GGGCTCTGATGCAAGCAAAGAACCTTTTCCCTAATACGTATCTAATTGTGGGAGTCTGCAGTGATGAGCT AACGCACAACTTCAAGGGCTTCACTGTGATGAACGAGAATGAGCGCTATGACGCCGTGCAGCACTGCCGC TACGTGGACGAGGTGGTGCGGAACGCCCCCTGGACTCTGACGCCTGAGTTCCTGGCAGAGCACCGGATTG ATTTTGTCGCCCATGACGACATCCCCTATTCTTCTGCAGGGAGCGATGATGTGTATAAGCACATCAAGGA CGCAGGCATGTTTGCTCCCACACAGAGGACAGAAGGTATCTCCACATCAGACATCATCACCCGCATTGTC CGTGACTATGATGTGTATGCAAGACGGAACCTACAGAGGGGCTACACTGCCAAGGAGCTCAATGTCAGCT TTATCAACGAGAAGAAATACCACTTGCAAGAACGAGTTGATAAAGTAAAGAAGAAAGTGAAAGATGTGGA GGAAAAGTCAAAAGAGTTTGTGCAGAAGGTGGAAGAGAAGAGCATCGACCTCATCCAGAAGTGGGAGGAG AAGTCCCGAGAGTTCATTGGAAGTTTCCTGGAAATGTTTGGTCCAGAAGGAGCACTGAAGCACATGCTGA AGGAGGGAAAAGGTCGGATGCTGCAGGCCATCAGTCCCAAGCAGAGCCCCAGCAGCAGCCCTACTCATGA GCGCTCCCCCTCCCCCTCCTTTCGGTGGCCCTTCTCTGGCAAGACTTCCCCATCTTCCTCCCCAGCAAGT CTTTCTAGGTGCAGGGCTGTGACCTGTGACATCAGCGAGGATGAAGAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226245 representing NM_001163159
Red=Cloning site Green=Tags(s) MDAQSSAKVNSRKRRKEAPGPNGATEEDGIPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEACRG TPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCR YVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKDAGMFAPTQRTEGISTSDIITRIV RDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEE KSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSFRWPFSGKTSPSSSPAS LSRCRAVTCDISEDEED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163159 |
ORF Size | 1101 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163159.1, NP_001156631.1 |
RefSeq Size | 4951 bp |
RefSeq ORF | 1104 bp |
Locus ID | 13026 |
UniProt ID | P49586 |
Gene Summary | Controls phosphatidylcholine synthesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR226245 | Pcyt1a (Myc-DDK-tagged) - Mouse phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a), transcript variant 1 |
CNY 3,656.00 |
|
MR226245L3 | Lenti ORF clone of Pcyt1a (Myc-DDK-tagged) - Mouse phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a), transcript variant 1 |
CNY 4,750.00 |
|
MR226245L4 | Lenti ORF clone of Pcyt1a (mGFP-tagged) - Mouse phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a), transcript variant 1 |
CNY 4,750.00 |