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Ptpra (NM_008980) Mouse Tagged ORF Clone
CAT＃: MG226249
- TrueORF®
Ptpra (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type A (Ptpra) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_008980" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ptpa; Ptpalpha; Rptpalpha; Rptra; Rptralpha |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226249 representing NM_008980
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCTGGTTCATTCTTGTCCTGTTTGGCAGTGGTCTAATACATGTTAGTGCCAACAATGCTACTA CAGTTTCACCTTCTTTAGGAACGACAAGATTAATTAAAACATCAACAACAGAATTGGCTAAGGAAGAGAA TAAAACCTCAAATTCAACCTCTTCAGTAATTTCTCTTTCTGTGGCACCAACATTCAGCCCAAACCTGACT CTGGAGCCCACCTATGTGACTACTGTTAATTCTTCACACTCTGACAATGGGACCAGGAGGGCAGCCAGCA CGGAATCTGGAGGCACTACCATTTCCCCGAACGGAAGCTGGCTTATTGAGAACCAGTTCACGGATGCCAT AACAGAACCCTGGGAGGGGAACTCCAGCACTGCAGCAACCACTCCAGAAACCTTCCCCCCGGCAGATGAG ACACCAATTATTGCGGTGATGGTGGCCCTGTCCTCTCTGCTAGTAATCGTGTTTATTATCATAGTTCTGT ACATGTTAAGGTTTAAGAAATACAAGCAAGCTGGGAGTCATTCCAACTCTTTCCGCCTGTCAAATGGCCG CACGGAGGATGTGGAGCCCCAAAGTGTACCACTTCTGGCCAGGTCCCCAAGCACCAACAGGAAGTACCCA CCACTGCCTGTGGACAAGCTGGAAGAGGAGATTAACCGGAGAATGGCTGATGACAATAAGCTCTTCAGAG AAGAATTCAACGCTCTCCCTGCTTGTCCTATCCAGGCCACCTGTGAGGCTGCCTCCAAGGAAGAAAACAA GGAAAAAAACCGCTATGTAAACATCCTGCCCTTTCTCTCTTTAGCTGTGAGCAAGGATGCAGTGAAAGCA CTGAACAAAACCACTCCATTGCTAGAAAGAAGGTTTATTGGGAAATCAAACTCCAGAGGCTGTCTCTCAG ATGACCACTCTAGAGTGCACCTGACACCTGTTGAAGGGGTCCCAGATTCTGATTACATCAACGCTTCATT CATTAATGGCTACCAGGAAAAGAACAAATTCATCGCTGCACAAGGACCAAAAGAAGAAACAGTGAATGAC TTCTGGAGAATGATATGGGAACAAAACACAGCTACTATTGTCATGGTGACCAACCTGAAGGAGAGAAAGG AGTGTAAATGTGCCCAATACTGGCCAGACCAAGGCTGCTGGACCTATGGGAATGTCCGTGTGTCTGTCGA GGATGTGACTGTTCTGGTGGACTACACAGTACGGAAATTCTGCATCCAGCAGGTGGGCGACGTGACCAAC AGGAAACCACAGCGCCTCATCACTCAGTTCCACTTCACCAGCTGGCCAGACTTTGGGGTGCCTTTCACCC CAATTGGCATGCTCAAGTTCCTCAAGAAGGTGAAGGCCTGTAACCCTCAGTACGCAGGGGCTATCGTGGT CCACTGCAGTGCAGGTGTAGGGCGCACTGGCACCTTTGTTGTCATCGATGCCATGCTGGACATGATGCAT TCGGAACGCAAAGTGGATGTATACGGGTTTGTGAGCCGGATCCGGGCCCAGCGCTGCCAGATGGTACAGA CAGACATGCAGTACGTCTTCATATACCAGGCCCTTCTGGAGCATTATCTGTATGGGGACACAGAACTGGA AGTGACTTCTCTAGAAACCCACCTACAAAAAATTTATAACAAGATCCCAGGGACGAGCAACAACGGGTTA GAGGAGGAGTTTAAGAAATTAACTTCAATCAAAATCCAGAATGACAAGATGCGCACGGGAAACCTTCCAG CCAACATGAAGAAGAACCGGGTTTTACAGATCATTCCATATGAATTTAACAGAGTGATCATTCCAGTCAA ACGAGGCGAAGAGAACACAGACTATGTGAACGCATCCTTCATTGATGGATACCGGCAGAAAGACTCCTAC ATTGCCAGCCAGGGCCCTCTTCTCCACACGATTGAGGACTTCTGGCGAATGATCTGGGAGTGGAAGTCCT GTTCTATCGTAATGCTGACAGAACTGGAAGAGAGAGGCCAGGAGAAGTGTGCCCAGTACTGGCCATCTGA TGGCCTGGTGTCCTATGGAGACATCACAGTTGAGCTGAAGAAGGAGGAGGAATGTGAAAGCTACACTGTC CGAGACCTCCTGGTCACCAACACCAGGGAGAACAAGAGTCGGCAAATCCGGCAGTTCCACTTCCACGGCT GGCCTGAGGTGGGCATCCCCAGCGACGGCAAGGGCATGATCAACATCATTGCAGCAGTGCAGAAGCAGCA GCAGCAGTCGGGGAACCATCCCATCACTGTGCACTGCAGTGCCGGGGCAGGACGGACAGGAACCTTCTGT GCCTTGAGCACAGTCCTGGAACGTGTGAAAGCAGAAGGAATTTTAGATGTCTTCCAAACTGTCAAGAGCC TGCGGCTGCAGAGGCCACACATGGTCCAGACACTGGAACAGTATGAATTCTGCTACAAGGTGGTACAGGA GTACATTGACGCCTTTTCAGATTATGCCAACTTCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226249 representing NM_008980
Red=Cloning site Green=Tags(s) MDSWFILVLFGSGLIHVSANNATTVSPSLGTTRLIKTSTTELAKEENKTSNSTSSVISLSVAPTFSPNLT LEPTYVTTVNSSHSDNGTRRAASTESGGTTISPNGSWLIENQFTDAITEPWEGNSSTAATTPETFPPADE TPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYP PLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPFLSLAVSKDAVKA LNKTTPLLERRFIGKSNSRGCLSDDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVND FWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNVRVSVEDVTVLVDYTVRKFCIQQVGDVTN RKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMH SERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGL EEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSY IASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTV RDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMINIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFC ALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008980 |
| ORF Size | 2487 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008980.2, NP_033006.2 |
| RefSeq Size | 3609 bp |
| RefSeq ORF | 2490 bp |
| Locus ID | 19262 |
| UniProt ID | P18052 |
| Gene Summary | Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (PubMed:22801373). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (PubMed:22801373).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221935 | Ptpra (untagged) - Mouse protein tyrosine phosphatase, receptor type, A (Ptpra), transcript variant 1, (10ug) |
CNY 10,640.00 |
|
| MR226249 | Ptpra (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, A (Ptpra), transcript variant 1 |
CNY 6,072.00 |
|
| MR226249L3 | Lenti ORF clone of Ptpra (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, A (Ptpra), transcript variant 1 |
CNY 7,700.00 |
|
| MR226249L4 | Lenti ORF clone of Ptpra (mGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, A (Ptpra), transcript variant 1 |
CNY 7,700.00 |
