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Nphs1 (NM_019459) Mouse Tagged ORF Clone
CAT＃: MG226368
- TrueORF®
Nphs1 (tGFP-tagged) - Mouse nephrosis 1 homolog nephrin (human) (Nphs1), (10ug)
ORF Plasmid: DDK
"NM_019459" in other vectors (4)
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CNY 14,720.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | nephrin; NephrinB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226368 representing NM_019459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCTAAGGAAGCCACAGTCAGAGGCCCCGGGGCAAGCCCAGTGCACAGAACTTGCCACCTGATTC CCCTGCTGCTCGCGGGAATGCTGACCACAGGCCTGGCCCAGTCGCCAGTCCCCACCTCAGCACCTCGAGG CTTCTGGGCTCTATCTGAAAACCTGACTGTGGTGGAAGGGTCGACAGTTAAGCTGTGGTGTGGCGTCAGG GCCCCCGGCAGTGTGGTGCAGTGGGCTAAGGATGGGCTGCTTCTGGGTCCAAACCCTAAGATTCCAGGCT TCCCAAGGTACAGCCTGGAAGGAGACAGTGCTAAAGGTGAGTTCCACCTGCTTATTGAAGCCTGTGACCT CAGCGATGATGCGGAGTACGAGTGCCAAGTCGGCCGCTCCGAGTTGGGTCCCGAGCTCGTGTCTCCCAGC GTAATCCTCTCCATCCTAGTTTCCCCCAAGGTGCTTCAGTTAACCCCTGAGGCAGGAAGCACAGTTACCT GGGTAGCTGGACAGGAATATGTGGTCACCTGTGTGTCTGGGGATGCAAAGCCAGCACCTGACATCATCTT CATCCAGGGTGGACGCACGGTAGAGGATGTCTCCTCCAGCGTGAATGAGGGATCAGAGGAGAAACTCTTC TTCACGGAAGCCGAAGCCAGGGTGACACCCCAGAGCTCAGATAATGGGCAGCTGCTAGTCTGCGAGGGGT CCAACCCAGCCTTGGCCACTCCCATAAAGGCTTCGTTCACCATGAATATCCTGTTCCCCCCAGGACCTCC TGTCATTGATTGGCCAGGCCTGAATGAGGGGCATGTACGGGCAGGGGAGAACCTGGAGCTACCCTGCATA GCCAGAGGTGGAAATCCACCTGCGACCCTGCAGTGGCTGAAGAATGGTAAACCAGTGTCCATAGCTTGGG GCACAGAGCATGCCCAGGCAGTGGCTCACAGTGTGCTGGTGATGACCGTTCGACCTGAAGACCACGGAGC TCGGCTCAGCTGTCAGTCCTACAACAGTGTGTCTGCAGAGACCCAGGAGCGAAGCATCACTCTGCAGGTC ACCTTTCCCCCCAGTGCCGTTACCATCCTGGGATCTACATCACAGTCTGAGAACAAAAATGTGACCCTTT GCTGCCTTACCAAGTCCAGTCGCCCACGGGTCCTGCTGCGATGGTGGTTGGGTGGACGGCAGTTGCTGCC CACGGATGAGACAGTCATGGATGGCCTGCATGGTGGCCACATCTCCATGTCCAATCTGACACTCTTGGTG AAGAGAGAAGACAATGGCCTGTCCCTCACCTGCGAAGCCTTCAGTGATGCCTTCAGCAAGGAGACCTTCA AGAAGTCACTCACCTTGAATGTAAAATACCCGGCCCAGAAGCTGTGGATTGAGGGACCCCCAGAGGGGCA GAGCATCCGGACTGGAACTCGGGTGAGGCTGGTATGCTTGGCCATTGGAGGCAACCCAGAGCCCTCCCTC ACCTGGCTTAAGGATTCGCGCCCGGTGAACGATCCTCGGCAGTCTCAGGAGCCCCGGCGTGTGCAGCTGG GCAGTGTGGAGAAGTCCGGCAGCACTTTCTCCCGCGAGCTGGTGCTGATCATAGGCCCGCCGGACAACCT AGCCAAGTTCTCCTGCAAGGCGGGTCAGCTCAGTGCGTCTACGCAGCTGGTGGTGCAGTTCCCCCCAACA AACCTGACCATCCTGGCCAACTCGTCCGCACTGCGCCCAGGCGACGCCTTGAACTTGACCTGCGTCAGCA TCAGCAGCAACCCTCCAGTTAACTTGTCTTTGGACAAGGAAGGAGAGAGGCTGGACGACGTGGCTGCAAA ACCCCAGAGCGCCCCATTCAAAGGCTCCGCTGCATCCAGGAGTGTTTTTCTTCGGGTGTCATCCCGAGAC CACGGTCACCGGGTTACCTGCCGGGCCCACAGCGAGGCACTTCGTGAAACCGTGAGCTCTTTCTACCGCC TCAACGTGTTATACCCTCCAGAGTTCCTGGGAGAGCAAGTGCGGGCAGTGACCGTGGTGGAGCAGGGCCA GGCACTGCTGCCTGTGTCCGTGTCTGCTAACCCCGCCCCCGAGGCCTTCAACTGGACCTTCCGAGGCTAC CGCCTCAGCCCAGCTGGGGGTCCCCGGCACCGCATCCTGTCTGGAGGGGCACTGCAGCTGTGGAATGTAA CCCGAGCTGACGATGGCTTTTATCAGCTACACTGCCAGAACTCGGAGGGCACCGCCGAGGCGCTGTTGAA GCTGGACGTGCATTATGCTCCCACCATCCGTGCCCTGAAGGACCCTACTGAGGTGAATGTTGGGGGTTCT GTGGATATAGTCTGCACCGTCGATGCCAATCCCATCCTCCCAGAGATGTTCAGCTGGGAGAGACTGGGGG AAGACGAGGAGGAACTGAACCTGGACGACATGGAGAAGATGTCAAAGGGATCCACAGGGCGTCTGCGGAT TCGCCAGGCCAAGCTGTCCCAGGCTGGCGCTTACCAGTGCATCGTGGACAACGGGGTGGCTCCCGCAGCC AGAGGACTGGTTCGTCTTGTTGTCCGATTTGCCCCCCAGGTGGATCATCCTACTCCCCTGACGAAAGTGG CTGCTGCTGGGGACAGCACCAGCTCAGCCACACTCCACTGCCGTGCCCGGGGGGTCCCCAACATCGACTT CACTTGGACCAAAAATGGGGTCCCTCTGGATCTCCAAGACCCCAGGTACACAGAGCACAAGTACCACCAG GGTGTGGTCCACAGCAGCCTCCTGACCATTGCTAATGTGTCTGCAGCCCAGGACTATGCCCTCTTCAAAT GCACAGCCACCAATGCCCTTGGTTCAGACCACACCAACATCCAGCTCGTCAGCATCAGCCGTCCTGACCC TCCACTGGGGCTGAAGGTTGTGAGCGTGAGCCCTCACTCGGTAGGACTGGAGTGGAAACCTGGCTTTGAT GGGGGTCTGCCTCAGAGGTTCCAAATCAGGTATGAGGCCCTCGAGAGCCCAGGATTCCTCTACATGGATG TCCTGCCTGCACAGGCCACCACCTTCACACTGACTGGGCTGAAGCCTTCTACACGGTACAGGATCTGGCT GTTGGCCAGCAATGCCCTGGGGGACAGTGGATTGACGGACAAGGGGATCCAGGTCTCCATCACTACCCCA GGCCTGGACCAGGCTCCTGAAGACACAGACCAGCCGCTGCCCACAGAGCAGCCTCCGGGACCCCCGAGGC TGCCCCTGCTGCCTGTGCTCTTTGCGGTTGGTGGTCTTCTGTTGCTTTCCAATGCCTCCTGTGTTGGGGG ACTCCTCTGGCGGAGAAGACTGAGGCGCCTTGCTGAGGAGATCTCAGAGAAGACAGAGGCAGGGTCGGAG GAGGATCGAATCAGGAATGAATATGAGGAGAGTCAGTGGACTGGGGACCGGGACACAAGAAGCTCCACGG TTAGCACAGCAGAAGTGGACCCACACTACTACTCCATGAGGGACTTCAGTCCCCAGCTTCCCCCAACACT GGAAGAGGTGTCATATCGCCAAGCCTTCACAGGTATTGAAGATGAGGACATGGCCTTCCCCGGACACCTG TATGACGAGGTGGAGAGAGTCTATGGCCCACCTGGGGTCTGGGGACCCCTCTATGATGAAGTACAAATGG ACCCCTATGACCTTCGCTGGCCTGAGGTCAAATATGAGGACCCAAGGGGAATCTACGACCAGGTGGCGGC AGACATGGATGCTGGGGAGCCTGGTTCTCTGCCCTTTGAGCTGAGGGGACATCTGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226368 representing NM_019459
Red=Cloning site Green=Tags(s) MGAKEATVRGPGASPVHRTCHLIPLLLAGMLTTGLAQSPVPTSAPRGFWALSENLTVVEGSTVKLWCGVR APGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPS VILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLF FTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCI ARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQV TFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLV KREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSL TWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPT NLTILANSSALRPGDALNLTCVSISSNPPVNLSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRD HGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGY RLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGS VDIVCTVDANPILPEMFSWERLGEDEEELNLDDMEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAA RGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQ GVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFD GGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTP GLDQAPEDTDQPLPTEQPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSE EDRIRNEYEESQWTGDRDTRSSTVSTAEVDPHYYSMRDFSPQLPPTLEEVSYRQAFTGIEDEDMAFPGHL YDEVERVYGPPGVWGPLYDEVQMDPYDLRWPEVKYEDPRGIYDQVAADMDAGEPGSLPFELRGHLV TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019459 |
| ORF Size | 3768 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019459.2, NP_062332.2 |
| RefSeq Size | 5661 bp |
| RefSeq ORF | 3771 bp |
| Locus ID | 54631 |
| UniProt ID | Q9QZS7 |
| Gene Summary | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224090 | Nphs1 (untagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1), (10ug) |
CNY 16,250.00 |
|
| MR226368 | Nphs1 (Myc-DDK-tagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1) |
CNY 13,120.00 |
|
| MR226368L3 | Lenti ORF clone of Nphs1 (Myc-DDK-tagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1) |
CNY 14,440.00 |
|
| MR226368L4 | Lenti ORF clone of Nphs1 (mGFP-tagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1) |
CNY 14,440.00 |
