Btrc (NM_001037758) Mouse Tagged ORF Clone
CAT#: MG226534
- TrueORF®
Btrc (tGFP-tagged) - Mouse beta-transducin repeat containing protein (Btrc) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001037758" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,650.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | b-TrCP; beta-TrCP; Beta-Trcp1; E3RS-IkappaB; E3RSIkappaB; Fbw1a; FWD1; HOS; Slimb |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226534 representing NM_001037758
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCGGCAGAGGCGGTGCTGCAGGAGAAAGCGCTTAAGTTTATGTGCTCTATGCCCAGGTCTCTGT GGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGC ACTCACAGCTTTCCAGAATTCCTCAGAGAGAGAAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATA CCAGAGAAGAATTCACTTAGACAGACTTACAACAGCTGTGCCAGGCTTTGCATAAACCAAGAGACAGTAT GTCTAACAAGCACTGCTATGAAGACTGAAAATTGTGTGGCCAAAGCCAAACTTGCCAATGGCACTTCCAG CATGATTGTGCCCAAGCAGCGGAAACTCTCAGCAAGCTATGAGAAGGAAAAGGAGCTGTGTGTCAAGTAT TTTGAGCAGTGGTCAGAGTCTGATCAAGTGGAATTTGTAGAACACCTTATATCCCAAATGTGTCACTACC AGCATGGGCACATCAACTCCTACCTAAAACCTATGCTGCAGAGGGATTTCATAACTGCACTGCCAGCACG GGGTCTGGACCACATCGCTGAGAACATTCTGTCATACTTGGACGCCAAGTCACTGTGTGCTGCTGAGCTC GTGTGCAAGGAATGGTACCGCGTGACGTCGGACGGCATGCTGTGGAAAAAGCTCATCGAGAGGATGGTCA GGACGGACTCTCTGTGGCGAGGCCTGGCAGAGCGCAGAGGCTGGGGACAGTACTTATTCAAAAACAAACC TCCTGATGAGAACGCTCCTCCCAACTCCTTTTATAGAGCGCTTTATCCTAAAATCATACAAGACATTGAG ACAATAGAGTCCAATTGGAGATGTGGGCGACATAGTTTACAGAGAATCCACTGCCGGAGTGAAACAAGTA AAGGGGTTTACTGTTTACAGTACGACGACCAGAAGATAGTCAGCGGCCTTCGAGACAACACCATCAAGAT CTGGGATAAAAGCACACTGGAATGCAAGCGGATTCTCACGGGCCACACGGGCTCCGTCCTGTGTCTGCAG TACGATGAGAGGGTGATCATCACAGGCTCCTCAGACTCCACCGTCAGAGTGTGGGATGTAAATGCAGGTG AGATGCTAAACACATTGATTCACCACTGTGAAGCCGTTCTGCACCTGCGCTTCAATAATGGCATGATGGT GACCTGTTCCAAAGACCGTTCCATCGCTGTGTGGGATATGGCTTCCCCAACTGACATCACCCTCAGGAGG GTGCTGGTGGGACACCGAGCTGCGGTCAATGTTGTAGACTTTGATGACAAGTACATCGTTTCTGCCTCTG GAGATAGAACCATAAAGGTGTGGAACACAAGTACCTGTGAATTCGTAAGGACCCTAAATGGGCACAAGCG TGGCATCGCCTGTTTGCAGTACAGAGACAGGCTGGTGGTGAGCGGCTCCTCTGACAACACCATCAGGCTG TGGGACATAGAGTGTGGAGCATGCCTGCGAGTGTTGGAGGGCCATGAGGAGTTGGTACGCTGCATTCGAT TTGATAACAAAAGGATAGTGAGCGGAGCCTATGATGGGAAAATTAAAGTGTGGGATCTTATGGCTGCTTT GGACCCGCGTGCTCCAGCAGGGACTCTCTGTCTGCGGACACTTGTGGAGCATTCTGGAAGAGTTTTCCGC CTCCAGTTTGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATTCTCATCTGGGACTTCCTGA ATGATCCAGCTGCTCACGCTGAACCGCCCCGCTCCCCTTCTCGGACATACACCTACATCTCCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226534 representing NM_001037758
Red=Cloning site Green=Tags(s) MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKII PEKNSLRQTYNSCARLCINQETVCLTSTAMKTENCVAKAKLANGTSSMIVPKQRKLSASYEKEKELCVKY FEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAEL VCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDENAPPNSFYRALYPKIIQDIE TIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKSTLECKRILTGHTGSVLCLQ YDERVIITGSSDSTVRVWDVNAGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRR VLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRL WDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLMAALDPRAPAGTLCLRTLVEHSGRVFR LQFDEFQIVSSSHDDTILIWDFLNDPAAHAEPPRSPSRTYTYISR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001037758 |
ORF Size | 1815 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037758.2, NP_001032847.2 |
RefSeq Size | 6358 bp |
RefSeq ORF | 1818 bp |
Locus ID | 12234 |
UniProt ID | Q3ULA2 |
Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins (PubMed:10097128, PubMed:16371461, PubMed:18782782, PubMed:9859996, PubMed:9990853, PubMed:21911472). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (By similarity). SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription (PubMed:9859996, PubMed:10097128). Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22' (PubMed:9859996, PubMed:10097128). SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (By similarity). SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1 (PubMed:21911472). Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2 (PubMed:18782782). May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase (By similarity). Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF (By similarity). Required for proteolytic processing of GLI3 (PubMed:16371461). Mediates ubiquitination of REST, thereby leading to its proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219602 | Btrc (untagged) - Mouse beta-transducin repeat containing protein (Btrc), transcript variant 1, (10ug) |
CNY 7,790.00 |
|
MR226534 | Btrc (Myc-DDK-tagged) - Mouse beta-transducin repeat containing protein (Btrc), transcript variant 1 |
CNY 6,080.00 |
|
MR226534L3 | Lenti ORF clone of Btrc (Myc-DDK-tagged) - Mouse beta-transducin repeat containing protein (Btrc), transcript variant 1 |
CNY 7,980.00 |
|
MR226534L4 | Lenti ORF clone of Btrc (mGFP-tagged) - Mouse beta-transducin repeat containing protein (Btrc), transcript variant 1 |
CNY 7,980.00 |