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Nlgn1 (NM_138666) Mouse Tagged ORF Clone
CAT＃: MG226624
- TrueORF®
Nlgn1 (tGFP-tagged) - Mouse neuroligin 1 (Nlgn1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_138666" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330415N05Rik; BB179718; mKIAA1070; NL1; Nlg1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226624 representing NM_138666
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTTCCCAGATGCATGTGGCCAAATTATGTTTGGAGAGCTATGATGGCATGTGTGGTCCACAGGG GATCCGGTGCCCCATTGACTCTCTGCTTGTTGGGATGTTTGCTACAGACTTTTCACGTACTCTCTCAAAA GTTGGATGATGTAGACCCATTGGTTACTACTAACTTTGGCAAGATTAGGGGAATTAAGAAAGAACTCAAT AATGAAATTTTGGGTCCTGTCATTCAGTTTCTTGGGGTTCCATATGCCGCTCCACCAACAGGAGAACATC GTTTCCAGCCTCCAGAACCACCATCTCCCTGGTCTGACATCCGGAACGCCACTCAGTTTGCTCCTGTATG TCCCCAGAATATCATTGATGGCAGATTGCCTGAAGTTATGCTTCCTGTGTGGTTCACTAATAACTTGGAT GTGGTTTCATCATACGTCCAAGACCAGAGTGAAGACTGTCTATACTTAAACATCTATGTCCCAACTGAAG ATGGTCCCCTTACAAAGAAACACACAGATGATTTAGGTGATAATGACGGTGCTGAAGATGAAGATATTCG GGACAGTGGGGGTCCCAAACCAGTGATGGTGTACATCCATGGCGGCTCTTACATGGAAGGTACTGGAAAT CTGTATGATGGGAGTGTCTTGGCAAGCTATGGCAATGTGATCGTCATCACAGTCAACTATCGGCTTGGGG TACTTGGCTTCTTGAGCACAGGGGATCAGGCTGCCAAAGGAAACTACGGGCTCCTTGACCTCATCCAGGC CCTAAGATGGACCAGCGAGAACATTGGGTTCTTTGGTGGTGACCCCTTGCGAATCACTGTGTTTGGATCA GGCGCTGGGGGTTCATGTGTCAACCTGCTGACTTTATCCCATTATTCTGAAGGTAACCGTTGGAGCAATT CAACCAAAGGACTTTTTCAACGAGCAATAGCTCAGAGTGGAACAGCCCTTTCCAGCTGGGCTGTTAGTTT CCAGCCTGCAAAATACGCTAGAATTCTGGCCACAAAAGTTGGCTGCAATGTTTCAGATACAGTAGAGTTA GTAGAATGCCTGCAGAAGAAGCCTTACAAAGAACTTGTTGATCAAGATGTTCAACCAGCCCGATACCACA TAGCCTTTGGACCTGTGATCGATGGTGATGTAATACCAGATGACCCTCAGATACTGATGGAACAAGGAGA GTTCCTCAACTATGATATAATGTTGGGAGTTAACCAAGGGGAAGGGTTGAAGTTTGTCGAAAATATAGTA GATAGTGATGATGGTGTATCAGCCAGTGATTTCGACTTTGCTGTTTCTAATTTTGTTGATAATTTATATG GATATCCTGAAGGCAAAGATGTTTTGAGAGAAACCATTAAATTCATGTATACTGACTGGGCTGATCGCCA TAATCCTGAAACTAGAAGGAAGACATTGTTGGCTTTGTTTACGGACCATCAATGGGTAGCACCTGCTGTG GCCACAGCAGACCTTCACTCGAACTTTGGCTCACCTACATACTTCTATGCCTTTTATCATCATTGCCAAA CAGATCAAGTTCCAGCTTGGGCTGATGCAGCTCATGGGGATGAGGTTCCCTATGTGTTGGGAATCCCCAT GATTGGCCCTACAGAGTTATTTCCTTGCAATTTCTCCAAGAATGATGTGATGTTGAGTGCAGTAGTAATG ACATACTGGACGAATTTTGCTAAAACTGGTGACCCAAATCAACCAGTTCCTCAAGACACAAAATTCATCC ATACCAAACCCAACCGCTTTGAAGAAGTAGCATGGACCAGATATTCCCAGAAAGACCAGCTTTATCTCCA TATTGGATTAAAACCGAGAGTTAAAGAGCATTACAGAGCCAATAAGGTAAATCTCTGGCTGGAGCTGGTA CCTCATCTGCATAATCTCAATGACATTTCTCAGTATACCTCGACAACAACTAAAGTGCCATCCACGGACA TCACTCTCAGACCTACAAGGAAGAATTCCACACCAGTCACATCAGCCTTTCCCACTGCCAAACAGGATGA TCCCAAGCAACAACCAAGCCCCTTCTCGGTGGATCAGAGGGACTACTCCACAGAGCTAAGTGTCACTATC GCAGTGGGGGCCTCTCTGCTGTTTCTCAACATCTTGGCTTTTGCAGCCCTGTACTACAAGAAGGATAAGA GGAGACATGATGTCCACCGGAGGTGCAGCCCTCAGCGCACGACCACCAACGACCTAACCCATGCTCCAGA AGAGGAAATTATGTCTCTCCAAATGAAGCACACTGACTTGGATCACGAGTGTGAGTCCATCCATCCACAT GAGGTGGTTCTTCGGACCGCCTGTCCCCCAGATTATACTCTAGCTATGAGGAGGTCACCTGATGATATTC CACTAATGACACCTAACACCATCACAATGATTCCCAACACTATACCAGGGATTCAGCCCTTACATACATT CAACACATTTACTGGAGGACAGAATAATACACTGCCCCATCCCCACCCACACCCCCATTCACATTCAACA ACCAGGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226624 representing NM_138666
Red=Cloning site Green=Tags(s) MALPRCMWPNYVWRAMMACVVHRGSGAPLTLCLLGCLLQTFHVLSQKLDDVDPLVTTNFGKIRGIKKELN NEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLD VVSSYVQDQSEDCLYLNIYVPTEDGPLTKKHTDDLGDNDGAEDEDIRDSGGPKPVMVYIHGGSYMEGTGN LYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGS GAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVEL VECLQKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIV DSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAV ATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVM TYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELV PHLHNLNDISQYTSTTTKVPSTDITLRPTRKNSTPVTSAFPTAKQDDPKQQPSPFSVDQRDYSTELSVTI AVGASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTTNDLTHAPEEEIMSLQMKHTDLDHECESIHPH EVVLRTACPPDYTLAMRRSPDDIPLMTPNTITMIPNTIPGIQPLHTFNTFTGGQNNTLPHPHPHPHSHST TRV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138666 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138666.4 |
| RefSeq Size | 5630 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 192167 |
| UniProt ID | Q99K10 |
| Gene Summary | Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (PubMed:23716671).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222019 | Nlgn1 (untagged) - Mouse neuroligin 1 (Nlgn1), transcript variant 1, (10ug) |
CNY 10,740.00 |
|
| MR226624 | Nlgn1 (Myc-DDK-tagged) - Mouse neuroligin 1 (Nlgn1), transcript variant 1 |
CNY 6,176.00 |
|
| MR226624L3 | Lenti ORF clone of Nlgn1 (Myc-DDK-tagged) - Mouse neuroligin 1 (Nlgn1), transcript variant 1 |
CNY 9,030.00 |
|
| MR226624L4 | Lenti ORF clone of Nlgn1 (mGFP-tagged) - Mouse neuroligin 1 (Nlgn1), transcript variant 1 |
CNY 9,030.00 |
