Myh6 (NM_001164171) Mouse Tagged ORF Clone
CAT#: MG226784
- TrueORF®
Myh6 (tGFP-tagged) - Mouse myosin heavy polypeptide 6 cardiac muscle alpha (Myh6) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001164171" in other vectors (2)
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CNY 15,580.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A830009F23Rik; AA517445; alpha-MHC; Myhc-a; Myhca |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226784 representing NM_001164171
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACGCCCAGATGGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCC TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT TAAGGCCAAGGTCGTGTCCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAGACGGTGACC ATAAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCACGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTATACCTA CTCAGGCCTCTTCTGCGTCACCGTCAACCCCTATAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCC GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCGGGGAAGACTGTGAA CACAAAACGTGTCATCCAGTACTTTGCCAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGAAAATCCT AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCCGCTCTGGAGGCCTTCGGCAACG CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAATTCATCAGGATCCACTTTGGAGCTACTGG AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCCGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT GAGAGGAACTACCACATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG TCACCAACAACCCATACGACTACGCCTTCGTCTCTCAGGGAGAGGTGTCCGTGGCCTCCATTGATGACTC TGAGGAGCTCTTGGCCACTGATAGTGCCTTTGATGTGCTGAGCTTCACGGCAGAGGAGAAGGCTGGTGTC TACAAGCTGACAGGGGCCATCATGCACTACGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CGGAGCCTGATGGCACAGAAGATGCTGACAAATCTGCCTACCTTATGGGGCTGAACTCAGCTGACCTGCT CAAGGGCCTGTGTCACCCTCGGGTGAAGGTGGGGAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAA GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA TCAACGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGA GATCTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGGAGTTTATCGACC TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTCGAGGA GGAGTGCATGTTCCCCAAGGCCTCAGACATGACCTTCAAGGCCAAGCTGTATGACAACCACCTGGGCAAA TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCCTTGGTCCACTATG CTGGCACCGTGGACTACAACATTATGGGCTGGCTGGAAAAGAACAAGGACCCACTCAATGAGACGGTGGT GGGTTTGTACCAGAAGTCCTCCCTCAAGCTCATGGCTACACTCTTCTCTACCTATGCTTCTGCTGATACC GGTGACAGTGGTAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAAACAGTGTCTGCTCTCCACC GGGAAAATCTGAACAAGCTGATGACAAACCTGAAGACCACCCACCCTCACTTTGTGCGCTGCATCATTCC CAACGAGCGAAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTG CTGGAGGGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGT ATCGCATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACT GCTGGGCTCCCTGGACATTGACCACAACCAATACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGC CTGCTGGGGCTGCTCGAGGAGATGCGAGATGAGAGGCTGAGCCGTATCATCACCAGAATCCAGGCCCAGG CCCGAGGGCAGCTCATGCGCATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTTATCCA GTGGAACATTCGGGCCTTCATGGGGGTCAAGAATTGGCCATGGATGAAGCTCTACTTCAAGATCAAACCG CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCAACATGAAGGAGGAGTTTGGGCGAGTCAAAGATG CACTGGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA TGACCTACAGCTCCAAGTGCAGGCGGAACAAGACAACCTCAATGATGCAGAGGAGCGCTGTGACCAGCTG ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA TGAACGCCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGAAGGATATTGA TGACCTGGAGCTGACGCTGGCCAAGGTGGAAAAGGAAAAGCATGCAACAGAGAACAAGGTTAAAAACCTA ACAGAGGAGATGGCTGGGCTGGATGAAATCATTGCCAAGCTGACCAAAGAGAAGAAAGCTCTGCAAGAAG CCCACCAGCAAGCCCTCGATGACCTGCAGGCTGAAGAAGACAAGGTCAACACGCTGACCAAGTCCAAAGT CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAAGTGCGCATGGACCTA GAGCGAGCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAATG ACAAGCTTCAGCTGGAAGAAAAGCTCAAGAAGAAAGAGTTCGACATCAGTCAGCAGAACAGTAAAATTGA GGACGAGCAGGCCCTGGCTCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG GAGGAGGAGCTGGAGGCAGAGCGCACAGCCCGGGCTAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGG AGCTGGAGGAGATCAGTGAGAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAATAA GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACG GCGGCGGCCCTGCGCAAGAAGCATGCTGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGC GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACAT GGAGCAGATCATCAAGGCCAAGGCCAACCTGGAGAAGGTGTCCCGGACACTGGAGGACCAGGCCAATGAG TACCGCGTGAAGCTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA CAGAGAACGGGGAGTTGGCTAGGCAACTGGAAGAAAAGGAGGCATTGATTTCCCAGCTGACCCGAGGCAA GCTCTCCTACACCCAGCAGATGGAGGACCTCAAGAGGCAACTGGAGGAGGAAGGCAAGGCCAAGAACGCC CTGGCCCACGCACTGCAATCATCCCGGCATGACTGTGACCTGCTGAGGGAACAGTATGAAGAAGAAATGG AGGCCAAGGCTGAGCTACAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA TGAGACGGATGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAG GATGCAGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGTTCCTCCCTGGAGAAGACCAAGCACAGGCTGC AGAATGAGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAA GCAGAGGAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAG TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTC TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA GCTGGGAGAAGGGGGGAAAAACGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG GAGCTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGTGCCC AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCA GGCCAAGCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGC AATGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCAGG CCAATAGAATAGCCTCAGAGGCACAGAAACACCTGAAGAATTCTCAAGCTCACTTGAAGGACACCCAGCT CCAGCTGGATGATGCTGTCCATGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAACGGCGCAAC AACCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGG CAGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAA CCAGAAGAAGAAGATGGAGTCAGACCTGACCCAACTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGT AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCAATGATGGCTGAGGAGCTGAAGAAGG AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCA CCGTCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCAGAGTCGGTGAAGGGCATGAGGA AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTAATGCGGCTGCA GGACCTGGTGGACAAGCTACAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAG GCCAACACCTACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACA TCGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGAAGATGCACGACGA GGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226784 representing NM_001164171
Red=Cloning site Green=Tags(s) MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVT IKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENP NANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLPEKSRVIFQLKA ERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGV YKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQ VYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWEFIDLGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGK SNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADT GDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGV LEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAG LLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKP LLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQL IKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNL TEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDL ERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEEL EEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANE YRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNA LAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQ DAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELE SSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKL ELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSR NEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRN NLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQEC RNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEAR VRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQ ANTYLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKKMHDEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164171 |
ORF Size | 5814 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164171.1, NP_001157643.1 |
RefSeq Size | 6079 bp |
RefSeq ORF | 5817 bp |
Locus ID | 17888 |
UniProt ID | Q02566 |
Gene Summary | Muscle contraction.[UniProtKB/Swiss-Prot Function] |
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SDS |