Nkx2-2 (NM_010919) Mouse Tagged ORF Clone
CAT#: MG227490
- TrueORF®
Nkx2 (tGFP-tagged) - Mouse NK2 transcription factor related locus 2 (Drosophila) (Nkx2-2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_010919" in other vectors (4)
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CNY 3,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Nkx-2.2; Nkx2.2; tinman |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227490 representing NM_010919
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGACCAACACAAAGACGGGGTTTTCAGTCAAGGACATCTTGGACCTTCCGGACACCAACGATG AAGACGGCTCGGTGGCCGAAGGGCCAGAGGAGGAGAGCGAAGGGCCGGAGCCCGCCAAGAGGGCCGGGCC GCTGGGGCAGGGCGCCCTGGACGCTGTGCAGAGCCTGCCCCTTAAGAGCCCTTTCTACGACAGCAGCGAC AACCCCTACACTCGCTGGCTGGCCAGCACCGAGGGCCTCCAATACTCCCTGCACGGGCTGGCGGCCAGCG CTCCCCCCCAAGACTCGAGCTCCAAGTCCCCAGAGCCCTCGGCTGACGAGTCACCGGACAATGACAAGGA GACCCAGGGCGGCGGGGGGGACGCAGGCAAGAAGCGGAAGCGCCGAGTGCTCTTCTCCAAAGCGCAGACC TACGAGCTGGAGCGGCGCTTCCGGCAGCAGCGGTACCTGTCGGCGCCCGAGCGCGAGCACCTGGCCAGCC TCATCCGTCTCACGCCGACACAGGTCAAGATCTGGTTCCAGAACCATCGCTACAAGATGAAACGTGCCCG GGCGGAGAAAGGTATGGAGGTGACGCCTCTGCCCTCGCCGCGCCGTGTGGCAGTGCCGGTCTTGGTCAGG GACGGCAAACCGTGCCACGCGCTCAAAGCCCAGGACCTGGCAGCCGCCACCTTCCAGGCAGGCATCCCCT TTTCCGCCTACAGCGCGCAGTCGCTGCAGCACATGCAGTACAACGCCCAGTACAGCTCGGCCAGCACCCC CCAGTACCCGACAGCACACCCCCTGGTCCAGGCCCAGCAGTGGACTTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227490 representing NM_010919
Red=Cloning site Green=Tags(s) MSLTNTKTGFSVKDILDLPDTNDEDGSVAEGPEEESEGPEPAKRAGPLGQGALDAVQSLPLKSPFYDSSD NPYTRWLASTEGLQYSLHGLAASAPPQDSSSKSPEPSADESPDNDKETQGGGGDAGKKRKRRVLFSKAQT YELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVR DGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQYSSASTPQYPTAHPLVQAQQWTW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010919 |
ORF Size | 819 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010919.1 |
RefSeq Size | 2049 bp |
RefSeq ORF | 822 bp |
Locus ID | 18088 |
UniProt ID | P42586 |
Gene Summary | Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (PubMed:11076772). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (PubMed:19759004).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209037 | Nkx2 (untagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR227490 | Nkx2 (Myc-DDK-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1 |
CNY 2,760.00 |
|
MR227490L3 | Lenti ORF clone of Nkx2 (Myc-DDK-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1 |
CNY 4,660.00 |
|
MR227490L4 | Lenti ORF clone of Nkx2 (mGFP-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1 |
CNY 4,660.00 |