N6amt1 (NM_026366) Mouse Tagged ORF Clone
CAT#: MG227618
- TrueORF®
N6amt1 (tGFP-tagged) - Mouse N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_026366" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5830445C04Rik; Hemk2; Pred28 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227618 representing NM_026366
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCCGAGTGTCCCCACGCCGTTGTACGGGCACGTGGGTCGCGGAGCCTTCCGCGACGTGTACG AGCCAGCGGAGGACACGTTCCTGTTACTGGACGCGCTCGAGGCGGCGGCGGCCGAGCTAGCAGGAGTGGA AATATGCCTTGAAGTAGGAGCAGGATCTGGTGTGGTGTCTGCATTCCTGGCCTCCATGATAGGTCCTCGG GCCTTATACATGTGCACTGATATCAACCCTGAGGCAGCCGCATGTACCTTGGAAACAGCACGCTGTAACA GAGTCCATGTTCAGCCAGTGATCACAGATTTGGTGCACGGCTTGCTGCCCAGACTGAAGGGGAAAGTAGA CCTGCTGGTGTTTAACCCCCCCTATGTAGTGACTCCGCCTGAAGAGGTAGGAAGTCGTGGAATAGAAGCA GCCTGGGCTGGCGGCAGAAACGGCCGGGAAGTCATGGACAGGTTCTTCCCACTGGCTCCAGAACTCCTCT CCCCAAGAGGGCTGTTCTACTTAGTTACCGTAAAAGAAAACAATCCCGAGGAAATCTTTAAAACAATGAA GACAAGAGGTCTGCAAGGGACCACAGCACTTTGCAGGCAAGCAGGCCAAGAAGCCCTGTCAGTCCTCAGG TTCAGCAAGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227618 representing NM_026366
Red=Cloning site Green=Tags(s) MAAPSVPTPLYGHVGRGAFRDVYEPAEDTFLLLDALEAAAAELAGVEICLEVGAGSGVVSAFLASMIGPR ALYMCTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVGSRGIEA AWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGTTALCRQAGQEALSVLR FSKS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026366 |
ORF Size | 642 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026366.2, NP_080642.1 |
RefSeq Size | 1791 bp |
RefSeq ORF | 645 bp |
Locus ID | 67768 |
UniProt ID | Q6SKR2 |
Gene Summary | Methyltransferase that can methylate both proteins and DNA, and to a lower extent, arsenic (PubMed:20606008, PubMed:26797129). Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif (PubMed:26797129). Methylates ETF1 on 'Gln-185'; ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated (PubMed:20606008, PubMed:26797129). Also acts as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine) (By similarity). N(6)-methyladenosine (m6A) DNA is significantly enriched in exonic regions and is associated with gene transcriptional activation (By similarity). May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid (By similarity). It however only plays a limited role in arsenic metabolism compared with AS3MT (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210622 | N6amt1 (untagged) - Mouse N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR227618 | N6amt1 (Myc-DDK-tagged) - Mouse N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1 |
CNY 2,400.00 |
|
MR227618L3 | Lenti ORF clone of N6amt1 (Myc-DDK-tagged) - Mouse N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1 |
CNY 4,750.00 |
|
MR227618L4 | Lenti ORF clone of N6amt1 (mGFP-tagged) - Mouse N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1 |
CNY 4,750.00 |