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Sap18 (NM_009119) Mouse Tagged ORF Clone
CAT＃: MR201109
- TrueORF®
Sap18 (Myc-DDK-tagged) - Mouse Sin3-associated polypeptide 18 (Sap18)
ORF Plasmid: tGFP
"NM_009119" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C530046K05Rik; D11Ertd539e; EMegR4; Sap18a; Sinbp1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201109 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGAGTCGCGCGTTACCCAGGAGGAAATTAAGAAGGAGCCGGAGAAGCCGATCGACCGCGAGA AGACCTGCCCGCTCCTGCTGCGGGTCTTCACCACCAACAACGGCCGCCACCACCGAATGGACGAGTTCTC CCGCGGGAACGTGCCTTCGAGCGAGCTGCAGATCTACACCTGGATGGATGCAACCTTGAAAGAACTGACT AGTTTAGTGAAGGAAGTCTACCCAGAAGCCAGAAAGAAGGGCACACACTTCAATTTTGCAATTGTTTTTA TGGATCTTAAAAGACCTGGATATCGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGGAAGGGCACTGA TGACTCCATGACCCTGCAGTCACAGAAGTTCCAAATAGGGGATTATCTGGACATAGCCATCACGCCTCCA AATCGGGCGCCGCCTTCGTCAGGGAGGATGAGACCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201109 protein sequence
Red=Cloning site Green=Tags(s) MAVESRVTQEEIKKEPEKPIDREKTCPLLLRVFTTNNGRHHRMDEFSRGNVPSSELQIYTWMDATLKELT SLVKEVYPEARKKGTHFNFAIVFMDLKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPP NRAPPSSGRMRPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009119 |
| ORF Size | 462 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009119.2, NP_033145.1 |
| RefSeq Size | 3472 bp |
| RefSeq ORF | 519 bp |
| Locus ID | 20220 |
| UniProt ID | O55128 |
| MW | 17.6 kDa |
| Gene Summary | Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200393 | Sap18 (untagged) - Mouse Sin3-associated polypeptide 18 (Sap18), (10ug) |
CNY 1,200.00 |
|
| MG201109 | Sap18 (tGFP-tagged) - Mouse Sin3-associated polypeptide 18 (Sap18) |
CNY 2,850.00 |
|
| MR201109L3 | Lenti ORF clone of Sap18 (Myc-DDK-tagged) - Mouse Sin3-associated polypeptide 18 (Sap18) |
CNY 4,750.00 |
|
| MR201109L4 | Lenti ORF clone of Sap18 (mGFP-tagged) - Mouse Sin3-associated polypeptide 18 (Sap18) |
CNY 4,750.00 |
