C530044N13Rik (BC034347) Mouse Tagged ORF Clone
CAT#: MR201448
- TrueORF®
C530044N13Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C530044N13 gene (cDNA clone MGC:27760 IMAGE:2654874)
ORF Plasmid: tGFP
"BC034347" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC27760 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201448 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGTCCATTCTACTTCGTCCAAGGCGCAGACACTCAGTTTGGACTGATGAAGGCCTGGTGCTCA ACTCCCAGTTCTTGTACGATGCCTCCAGGTGCCCCGCACTGAAGCAGGCCCAGGATCACTGGCTGGACCA ACAGCTGAACATTGCAGAGCAGAAACAGTGCCAGCACGCCATTGTCTTCCAGCATATCCCGCTATTCCTG CAGAGTATTGATGAAGATGACGACTACTTCAACCTCACTAAGACCGTCCGTAAGGAACTGGCCGAAAAGC TCACCAGAGCAGGTATCAGAGCTGTGTTCTCCGGGCACTACCACAGGAATGCCGGGGGAACCTACCAGAA TCTTGACATGGTAGTTTCATCTGCTATCGGGTGCCAGCTGGGCAAAGACACCCACGGGCTCCGTGTGGTG GCCATCACTGCGGAGAAGATTGTTCATCGTTACTACAGTTTAGATGAGCTGAGCCAGGGTGGGGTCGAGG AGGACCTGAAGGAGCTGCTGAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201448 protein sequence
Red=Cloning site Green=Tags(s) MERSILLRPRRRHSVWTDEGLVLNSQFLYDASRCPALKQAQDHWLDQQLNIAEQKQCQHAIVFQHIPLFL QSIDEDDDYFNLTKTVRKELAEKLTRAGIRAVFSGHYHRNAGGTYQNLDMVVSSAIGCQLGKDTHGLRVV AITAEKIVHRYYSLDELSQGGVEEDLKELLKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC034347 |
ORF Size | 516 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC034347, AAH34347 |
RefSeq Size | 2343 bp |
RefSeq ORF | 518 bp |
Locus ID | 223978 |
MW | 19.7 kDa |
Gene Summary | Protein phosphatase that dephosphorylates AKT family kinase specifically at 'Ser-473', blocking cell cycle progression and promoting cell apoptosis. May play an inhibitory role in glucose uptake by adipocytes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC206981 | C530044N13Rik (untagged) - Mouse RIKEN cDNA C530044N13 gene (cDNA clone MGC:27760 IMAGE:2654874), (10ug) |
CNY 3,230.00 |
|
MG201448 | C530044N13Rik (tGFP-tagged) - Mouse RIKEN cDNA C530044N13 gene (cDNA clone MGC:27760 IMAGE:2654874) |
CNY 2,850.00 |
|
MR201448L3 | Lenti ORF clone of C530044N13Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C530044N13 gene (cDNA clone MGC:27760 IMAGE:2654874) |
CNY 4,750.00 |
|
MR201448L4 | Lenti ORF clone of C530044N13Rik (mGFP-tagged) - Mouse RIKEN cDNA C530044N13 gene (cDNA clone MGC:27760 IMAGE:2654874) |
CNY 4,750.00 |
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