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Cirbp (NM_007705) Mouse Tagged ORF Clone
CAT＃: MR201471
- TrueORF®
Cirbp (Myc-DDK-tagged) - Mouse cold inducible RNA binding protein (Cirbp)
ORF Plasmid: tGFP
"NM_007705" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cirp; R74941 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCAGATGAAGGCAAGCTTTTCGTGGGAGGACTCAGCTTCGACACCAACGAGCAGGCGCTGGAGC AGGTCTTCTCCAAGTATGGGCAGATCTCCGAAGTGGTGGTGGTAAAGGACAGGGAGACCCAGCGATCCCG AGGCTTTGGGTTTGTCACCTTTGAAAATATCGATGACGCTAAGGACGCCATGATGGCTATGAATGGGAAG TCTGTGGACGGGCGGCAGATCAGAGTTGACCAGGCTGGCAAGTCTTCTGACAACCGGTCCCGAGGATACC GGGGTGGCTCTGCTGGAGGCCGGGGCTTTTTCCGTGGGGGACGAAGCCGGGGCCGAGGGTTCTCCAGAGG AGGAGGAGACCGGGGCTATGGAGGTGGCCGCTTTGAGTCCCGGAGTGGGGGTTATGGAGGCTCCAGAGAC TACTATGCCAGCCGGAGTCAGGGTGGCAGCTATGGTTATCGGAGCTCGGGAGGGTCCTACAGAGACAGCT ATGACAGTTATGCTACACACAACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201471 protein sequence
Red=Cloning site Green=Tags(s) MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGK SVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGYGGGRFESRSGGYGGSRD YYASRSQGGSYGYRSSGGSYRDSYDSYATHNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007705 |
| ORF Size | 519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007705.3 |
| RefSeq Size | 1256 bp |
| RefSeq ORF | 519 bp |
| Locus ID | 12696 |
| UniProt ID | P60824 |
| MW | 18.6 kDa |
| Gene Summary | Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3' UTRs) of stress-responsive transcripts RPA2 and TXN.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203526 | Cirbp (untagged) - Mouse cold inducible RNA binding protein (Cirbp), (10ug) |
CNY 2,400.00 |
|
| MG201471 | Cirbp (tGFP-tagged) - Mouse cold inducible RNA binding protein (Cirbp) |
CNY 2,850.00 |
|
| MR201471L3 | Lenti ORF clone of Cirbp (Myc-DDK-tagged) - Mouse cold inducible RNA binding protein (Cirbp) |
CNY 4,750.00 |
|
| MR201471L4 | Lenti ORF clone of Cirbp (mGFP-tagged) - Mouse cold inducible RNA binding protein (Cirbp) |
CNY 4,750.00 |
