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Map2k3 (BC007467) Mouse Tagged ORF Clone
CAT＃: MR201496
- TrueORF®
Map2k3 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase 3 (cDNA clone MGC:5915 IMAGE:3497714)
ORF Plasmid: tGFP
"BC007467" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MEK3, MKK3, mMKK3b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201496 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGCCCGCCGCGAGCCCGCCGGCCAGCTTGCCTCAGACCAAAGGAAAATCCAAAAGGAAAAAGG ACTTACGGATATCCTGCGTGTCCAAGCCACCTGTGTCCAACCCCACACCCCCCCGGAACCTGGACTCCCG GACCTTCATCACTATCGGAGACAGAAACTTCGAAGTGGAGGCTGATGACTTGGTGACCATCTCAGAGCTG GGTCGTGGAGCCTATGGGGTGGTAGAGAAAGTGCGGCATGCTCAGAGTGGTACCATCATGGCTGTCAAGC GCATCCGGGCCACAGTGAACACACAGGAGCAGAAACGTCTGCTTATGGACCTAGACATCAACATGCGCAC GGTCGACTGCTTCTACACTGTCACCTTCTATGGTGCCCTCTTCAGAGAGGGGGATGTATGGATCTGCATG GAGCTCATGGACACTTCCCTGGATAAGTTCTACCGGAAGGTGCTGGAGAAGAACATGAAAATTCCGGAAG ACATCCTGGGGGAGATCGCTGTGTCTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201496 protein sequence
Red=Cloning site Green=Tags(s) MESPAASPPASLPQTKGKSKRKKDLRISCVSKPPVSNPTPPRNLDSRTFITIGDRNFEVEADDLVTISEL GRGAYGVVEKVRHAQSGTIMAVKRIRATVNTQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICM ELMDTSLDKFYRKVLEKNMKIPEDILGEIAVSM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC007467 |
| ORF Size | 519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC007467, AAH07467 |
| RefSeq Size | 2096 bp |
| RefSeq ORF | 521 bp |
| Locus ID | 26397 |
| MW | 19.5 kDa |
| Gene Summary | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MAP2K3 is associated with body mass index in American Indians and Caucasians and may mediate hypothalamic inflammation
,null,
Human Molecular Genetics
,PubMed ID 23825110
[Map2k3]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207024 | Map2k3 (untagged) - Mouse mitogen activated protein kinase kinase 3 (cDNA clone MGC:5915 IMAGE:3497714), (10ug) |
CNY 3,230.00 |
|
| MG201496 | Map2k3 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 3 (cDNA clone MGC:5915 IMAGE:3497714) |
CNY 2,850.00 |
|
| MR201496L3 | Lenti ORF clone of Map2k3 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase 3 (cDNA clone MGC:5915 IMAGE:3497714) |
CNY 4,750.00 |
|
| MR201496L4 | Lenti ORF clone of Map2k3 (mGFP-tagged) - Mouse mitogen activated protein kinase kinase 3 (cDNA clone MGC:5915 IMAGE:3497714) |
CNY 4,750.00 |
