Tmem47 (NM_138751) Mouse Tagged ORF Clone
CAT#: MR201624
- TrueORF®
Tmem47 (Myc-DDK-tagged) - Mouse transmembrane protein 47 (Tmem47), transcript variant 2
ORF Plasmid: tGFP
"NM_138751" in other vectors (3)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3010015F07Rik; AI115536; BCMP1; Tm4sf10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201624 representing NM_138751
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCTGGCAGCGGCATGGAAGAGGTGCGAGTGTCGGTGCTGACCCCGCTCAAGCTGGTCGGGC TGGTGTGCATCTTCTTGGCGCTGTGTCTGGATCTGGGAGCTGTTCTGAGCCCGGCCTGGGTCACCGCGGA CCACCAGTACTACCTGTCCCTGTGGGAGTCCTGCCGGAAACCCGCCAACCTGGATATCTGGCATTGCGAA TCCACTCTCGGCAGCGATTGGCAGATTGCTACTCTGGCTTTACTCCTGGGCGGTGCTGCCATCATTCTCA TTGCATTCCTGGTGGGTTTGATTTCAATCTGTGTGGGATCACGAAGGCGCTTCTACAGACCTGTTGCTGT CATGCTTTTTGCAGCAGTTGTTTTGCAGGTCTGCAGCCTGGTTCTTTATCCAATCAAGTTCATTGAAACT GTGAGCTTGAAAATTTACCACGAGTTCAATTGGGGTTATGGCCTGGCCTGGGGTGCAACTATATTTTCAT TTGGGGGTGCCATTCTTTATTGCCTGAACCCTAAGAACTATGAAGACTACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201624 representing NM_138751
Red=Cloning site Green=Tags(s) MASAGSGMEEVRVSVLTPLKLVGLVCIFLALCLDLGAVLSPAWVTADHQYYLSLWESCRKPANLDIWHCE STLGSDWQIATLALLLGGAAIILIAFLVGLISICVGSRRRFYRPVAVMLFAAVVLQVCSLVLYPIKFIET VSLKIYHEFNWGYGLAWGATIFSFGGAILYCLNPKNYEDYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_138751 |
ORF Size | 543 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138751.2, NP_620090.1 |
RefSeq Size | 4082 bp |
RefSeq ORF | 546 bp |
Locus ID | 192216 |
UniProt ID | Q9JJG6 |
MW | 20.4 kDa |
Gene Summary | Regulates cell junction organization in epithelial cells. May play a role in the transition from adherens junction to tight junction assembly. May regulate F-actin polymerization required for tight junctional localization dynamics and affect the junctional localization of PARD6B (PubMed:26990309). During podocyte differentiation may negatively regulate activity of FYN and subsequently the abundance of nephrin (PubMed:21881001).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG201624 | Tmem47 (tGFP-tagged) - Mouse transmembrane protein 47 (Tmem47) |
CNY 2,850.00 |
|
MR201624L3 | Lenti ORF clone of Tmem47 (Myc-DDK-tagged) - Mouse transmembrane protein 47 (Tmem47), transcript variant 2 |
CNY 4,750.00 |
|
MR201624L4 | Lenti ORF clone of Tmem47 (mGFP-tagged) - Mouse transmembrane protein 47 (Tmem47), transcript variant 2 |
CNY 4,750.00 |