Rybp (NM_019743) Mouse Tagged ORF Clone
CAT#: MR202718
- TrueORF®
Rybp (Myc-DDK-tagged) - Mouse RING1 and YY1 binding protein (Rybp)
ORF Plasmid: tGFP
"NM_019743" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2410018J24Rik; DEDAF; YEAF1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202718 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGGCGACAAGAAGAGCCCGACCAGGCCAAAAAGACAAGCGAAACCTGCCGCAGACGAAGGCT TTTGGGATTGTAGCGTCTGCACCTTTAGGAACAGCGCCGAAGCCTTTAAATGCAGCATCTGCGATGTGCG GAAAGGCACCTCCACCAGGAAACCTCGCATCAATTCTCAGCTGGTGGCACAGCAGGTGGCACAGCAGTAC GCCACTCCACCTCCCCCTAAGAAGGAGAAGAAGGAGAAGGTCGAAAAGCCTGACAAAGAAAAGCCAGAGA AAGACAAGGACATTAGCCCCAGTGTCACCAAGAAAAACACCAACAAGAAAACAAAACCAAAGTCTGATAT TCTGAAAGATCCTCCTAGTGAAGCTAACAGCATACAGTCTGCTAACGCTACAACAAAGACCAGCGAAACA AACCACACCTCAAGGCCCCGGCTGAAGAATGTGGACAGGAGCACCGCACAGCAGTTGGCAGTAACTGTGG GCAACGTCACCGTCATTATCACAGACTTTAAGGAAAAGACTCGCTCCTCCTCCACATCCTCTTCCACAGT GACCTCCAGTGCAGGGTCAGAACAGCAGAACCAGAGCAGCTCGGGCTCAGAGAGCACAGACAAAGGCTCC TCCCGCTCCTCCACGCCAAAGGGCGACATGTCAGCAGTGAATGATGAATCTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202718 protein sequence
Red=Cloning site Green=Tags(s) MTMGDKKSPTRPKRQAKPAADEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQY ATPPPPKKEKKEKVEKPDKEKPEKDKDISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSET NHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGS SRSSTPKGDMSAVNDESF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019743 |
ORF Size | 687 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019743.3, NP_062717.2 |
RefSeq Size | 4376 bp |
RefSeq ORF | 687 bp |
Locus ID | 56353 |
UniProt ID | Q8CCI5 |
MW | 24.8 kDa |
Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:22325148, PubMed:28596365). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (PubMed:28596365). May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (PubMed:22325148, PubMed:28596365). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (By similarity). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:17874297). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (By similarity). May bind to DNA (PubMed:19170609). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207520 | Rybp (untagged) - Mouse RING1 and YY1 binding protein (Rybp), (10ug) |
CNY 3,990.00 |
|
MG202718 | Rybp (tGFP-tagged) - Mouse RING1 and YY1 binding protein (Rybp) |
CNY 2,850.00 |
|
MR202718L3 | Lenti ORF clone of Rybp (Myc-DDK-tagged) - Mouse RING1 and YY1 binding protein (Rybp) |
CNY 4,750.00 |
|
MR202718L4 | Lenti ORF clone of Rybp (mGFP-tagged) - Mouse RING1 and YY1 binding protein (Rybp) |
CNY 4,750.00 |