Pih1d2 (BC019481) Mouse Tagged ORF Clone

CAT#: MR202763

  • TrueORF®

Pih1d2 (Myc-DDK-tagged) - Mouse PIH1 domain containing 2 (cDNA clone MGC:28515 IMAGE:4190604)

ORF Plasmid: DDK tGFP



  "BC019481" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,280.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2700059L22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202763 representing BC019481
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAAGGATTCCCGCTCCGCAGTCAGCCACTCGTCCTGTGCCTATGCTTATACCATCATTGATGTTG
CCTACAATCCTGGTGTTCTGCAAGCGGCAGAAAAAGACCAAGGGATCAAAGATCAGTTAATAAGAATGGC
CATGCTGTGCATCGAGGAGCGACTCCAGTTCACACTTGCACATTCCTACCATTTAACTAGTTTTAGACTG
AAAGGAAGCATCCAGAGAATGAAAGAAAGTCTGATGGGCATTAAAACTGACTTCCCAGACCTAAAAGCGA
TGACGAGAACAGAAAATACTCTTGCGAGGATCAGAAGCAGCACTGTGAGTGAACCTAATCACCTTCCTGA
GGTGCTATTGACAAAAAAGCAAGCGTCGGCCAAAGGACGATGTCTTATAGAAGAGATTTCTAGTTCGGAA
ATCCAGGTGGAGGTTAAGAAACCTGCGTACGAATTAAAGGTTGTAAAGGATCGGAACGAGAAACCTCTGA
AAATTGAGCTGAAAGTCGAGTTACCCGGGATTAAGTCAGTCTCTCTCTGTGAACTTAGTGTTTCTGAGGT
TGATATATTGATTGAGGTCTCTGAGATGTACCGATTATGCCTGAATCTTCCAGAATCGATTAATACAGAA
ATGACTACAGCAAAATTTGTCAAAAATAAATCTGCATTAATCATTACAATGCCCCTGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202763 representing BC019481
Red=Cloning site Green=Tags(s)

MGKDSRSAVSHSSCAYAYTIIDVAYNPGVLQAAEKDQGIKDQLIRMAMLCIEERLQFTLAHSYHLTSFRL
KGSIQRMKESLMGIKTDFPDLKAMTRTENTLARIRSSTVSEPNHLPEVLLTKKQASAKGRCLIEEISSSE
IQVEVKKPAYELKVVKDRNEKPLKIELKVELPGIKSVSLCELSVSEVDILIEVSEMYRLCLNLPESINTE
MTTAKFVKNKSALIITMPLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC019481
ORF Size 690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019481.1
RefSeq Size 1171 bp
RefSeq ORF 692 bp
Locus ID 72614
MW 42.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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