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Tmed9 (BC049282) Mouse Tagged ORF Clone
CAT＃: MR202845
- TrueORF®
Tmed9 (Myc-DDK-tagged) - Mouse transmembrane emp24 protein transport domain containing 9 (cDNA clone MGC:58875 IMAGE:2648375), complete
ORF Plasmid: tGFP
"BC049282" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2400003B06Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202845 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGTGCGAGGCGTGCGGGTTGTCGGAAGCTCACCGGGTCTCTTGCTGGGGAGAGGGATGCGGG CCTTCCTGCTACTGCTGTGGCTTGCAGCCCGCGGAAGCGCGCTGTATTTCCACATCGGAGAAACAGAAAA GAAGTGCTTCATTGAGGAGATTCCGGACGAGACCATGGTCATCGGAAATTACCGGACGCAGCTGTATGAC AAACAGCGGGAGGAGTACCAGCCAGCCACCCCTGGGCTTGGCATGTTCGTAGAGGTAAAAGACCCCGAGG ACAAGGTCATCCTGGCCCGGCAGTATGGCTCTGAGGGCAGGTTCACCTTCACCTCCCACACCCCTGGTGA GCACCAGATCTGTCTTCACTCAAATTCCACCAAGTTCTCCCTCTTTGCCGGAGGGATGCTGAGGGTTCAC CTTGACATCCAAGTGGGCGAACATGCAAACGACTATGCAGAAATTGCTGCCAAGGACAAGCTGAGCGAGC TGCAGCTGAGGGTGCGACAGCTGGTGGAGCAAGTGGAGCAGATCCAAAAGGAGCAGAACTACCAGCGGTG GCGAGAAGAGCGCTTCAGACAAACCAGCGAAAGCACCAACCAGCGAGTGCTATGGTGGTCCATCCTGCAG ACTCTCATCCTCGTGGCCATTGGCGTCTGGCAGATGCGGCACCTCAAGAGCTTTTTTGAAGCCAAGAAGT TGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202845 protein sequence
Red=Cloning site Green=Tags(s) MAAVRGVRVVGSSPGLLLGRGMRAFLLLLWLAARGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYD KQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVH LDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQ TLILVAIGVWQMRHLKSFFEAKKLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049282 |
| ORF Size | 705 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049282, AAH49282 |
| RefSeq Size | 1411 bp |
| RefSeq ORF | 707 bp |
| Locus ID | 67511 |
| MW | 27.1 kDa |
| Gene Summary | Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202845 | Tmed9 (tGFP-tagged) - Mouse transmembrane emp24 protein transport domain containing 9 (cDNA clone MGC:58875 IMAGE:2648375), complete |
CNY 2,850.00 |
|
| MR202845L3 | Lenti ORF clone of Tmed9 (Myc-DDK-tagged) - Mouse transmembrane emp24 protein transport domain containing 9 (cDNA clone MGC:58875 IMAGE:2648375), complete |
CNY 4,750.00 |
|
| MR202845L4 | Lenti ORF clone of Tmed9 (mGFP-tagged) - Mouse transmembrane emp24 protein transport domain containing 9 (cDNA clone MGC:58875 IMAGE:2648375), complete |
CNY 4,750.00 |
