Gzmb (NM_013542) Mouse Tagged ORF Clone
CAT#: MR203106
- TrueORF®
Gzmb (Myc-DDK-tagged) - Mouse granzyme B (Gzmb)
ORF Plasmid: tGFP
"NM_013542" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI553453; CCP; CCP-1/C1; CCP-1/C11; CCP1; Ctl; Ctla; Ctla-1; Ctla1; GZB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203106 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATCCTCCTGCTACTGCTGACCTTGTCTCTGGCCTCCAGGACAAAGGCAGGGGAGATCATCGGGG GACATGAAGTCAAGCCCCACTCTCGACCCTACATGGCCTTACTTTCGATCAAGGATCAGCAGCCTGAGGC GATATGTGGGGGCTTCCTTATTCGAGAGGACTTTGTGCTGACTGCTGCTCACTGTGAAGGAAGTATAATA AATGTCACTTTGGGGGCCCACAACATCAAAGAACAGGAGAAGACCCAGCAAGTCATCCCTATGGTAAAAT GCATTCCCCACCCAGACTATAATCCTAAGACATTCTCCAATGACATCATGCTGCTAAAGCTGAAGAGTAA GGCCAAGAGGACTAGAGCTGTGAGGCCCCTCAACCTGCCCAGGCGCAATGTCAATGTGAAGCCAGGAGAT GTGTGCTATGTGGCTGGTTGGGGAAGGATGGCCCCAATGGGCAAATACTCAAACACGCTACAAGAGGTTG AGCTGACAGTACAGAAGGATCGGGAGTGTGAGTCCTACTTTAAAAATCGTTACAACAAAACCAATCAGAT ATGTGCGGGGGACCCAAAGACCAAACGTGCTTCCTTTCGGGGGGATTCTGGAGGCCCGCTTGTGTGTAAA AAAGTGGCTGCAGGCATAGTTTCCTATGGATATAAGGATGGTTCACCTCCACGTGCTTTCACCAAAGTCT CGAGTTTCTTATCCTGGATAAAGAAAACAATGAAAAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203106 protein sequence
Red=Cloning site Green=Tags(s) MKILLLLLTLSLASRTKAGEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSII NVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGD VCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCK KVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013542 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013542.3 |
RefSeq Size | 1418 bp |
RefSeq ORF | 744 bp |
Locus ID | 14939 |
UniProt ID | P04187 |
MW | 27.5 kDa |
Gene Summary | This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200360 | Gzmb (untagged) - Mouse granzyme B (Gzmb), (10ug) |
CNY 2,400.00 |
|
MG203106 | Gzmb (tGFP-tagged) - Mouse granzyme B (Gzmb) |
CNY 2,850.00 |
|
MR203106L3 | Lenti ORF clone of Gzmb (Myc-DDK-tagged) - Mouse granzyme B (Gzmb) |
CNY 4,750.00 |
|
MR203106L4 | Lenti ORF clone of Gzmb (mGFP-tagged) - Mouse granzyme B (Gzmb) |
CNY 4,800.00 |