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Neil1 (BC043297) Mouse Tagged ORF Clone
CAT＃: MR203232
- TrueORF®
Neil1 (Myc-DDK-tagged) - Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651)
ORF Plasmid: tGFP
"BC043297" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810450N13Rik; Nei1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203232 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGAGGGCCCAGAGCTGCACCTGGCCAGCCACTTTGTGAATGAGACATGTAAGGGGCTGGTATTTG GTGGGTGTGTGGAGAAGTCCTCTGTCAGCCGGAACCCGGAGGTGCCCTTTGAGAGCAGTGCCTACCACAT CTCAGCTTTAGCCCGAGGCAAGGAGCTGCGCTTGACATTGAGCCCCCTGCCTGGGTCCCAGCCCCCTCAG AAGCCACTGTCCCTTGTCTTCCGCTTTGGGATGTCAGGATCCTTCCAGCTGGTACCCGCAGAGGCACTGC CCCGCCACGCCCATCTACGTTTTTACACAGCCCCACCTGCTCCCCGGCTTGCCCTTTGCTTCGTAGACAT CCGTCGCTTTGGCCACTGGGATCCTGGGGGTGAATGGCAACCAGGCCGTGGACCCTGTGTCTTGCTGGAG TATGAACGGTTCAGAGAGAACGTACTTCGGAACCTATCAGACAAAGCCTTTGACCGGCCCATCTGCGAGG CCTTGTTGGACCAGAGGTTCTTCAATGGCATTGGCAACTATCTGCGGGCAGAGATCCTGTACCGGCTGAA GATCCCTCCTTTTGAGAAGGCTCGTACAGTTCTAGAGGCCCTGCAACAGTGCCGGCCGAGCCCAGAGCTG ACCCTGAGCCAGAAGATCAAGGCCAAACTACAGAACCCAGACCTGCTGGAACTGTGTCACTTGGTGCCCA AGGAAGTGGTTCAGCTGGGTGAGGCCTGGGGAGGTCAAGATGGCCGGCGGCCTCTACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203232 protein sequence
Red=Cloning site Green=Tags(s) MPEGPELHLASHFVNETCKGLVFGGCVEKSSVSRNPEVPFESSAYHISALARGKELRLTLSPLPGSQPPQ KPLSLVFRFGMSGSFQLVPAEALPRHAHLRFYTAPPAPRLALCFVDIRRFGHWDPGGEWQPGRGPCVLLE YERFRENVLRNLSDKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARTVLEALQQCRPSPEL TLSQKIKAKLQNPDLLELCHLVPKEVVQLGEAWGGQDGRRPLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC043297 |
| ORF Size | 759 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC043297, AAH43297 |
| RefSeq Size | 1857 bp |
| RefSeq ORF | 761 bp |
| Locus ID | 72774 |
| MW | 28.5 kDa |
| Gene Summary | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217894 | Neil1 (untagged) - Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651), (10ug) |
CNY 6,940.00 |
|
| MG203232 | Neil1 (tGFP-tagged) - Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651) |
CNY 2,850.00 |
|
| MR203232L3 | Lenti ORF clone of Neil1 (Myc-DDK-tagged) - Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651) |
CNY 4,750.00 |
|
| MR203232L4 | Lenti ORF clone of Neil1 (mGFP-tagged) - Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651) |
CNY 4,750.00 |
