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Trmt12 (NM_026642) Mouse Tagged ORF Clone
CAT＃: MR203250
- TrueORF®
Trmt12 (Myc-DDK-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12)
"NM_026642" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4632406N01Rik; AI449275; Tyw2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203250 representing NM_026642
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAGAGTGTGAGGAGTCTGTAGTTGTCGCAGTTGTGACTGAGCCCCGGTTCACGCAGAGATACC GAGACTACCTGGAGGAGCAGAAACTCTTGGACAGGCTGCACCGGGTGGCGAAGCTGCGGGATGGCGCGGT GGCTCTGCCGGTGCTGGCGGAAAGCCTTTCTGAGCAGCATCTGCAGGAGCTGAGGGATCGCGTGGCCCCA GGCAGCACTTGTGTGCTGACGCGGCTCCCGGATCCTCTTCCTTCCAAAAAGGCCCGGGTTCGTTCACCTG CTCAGATACTGTGTCTGGAGGTGAGGCGTTGGGTGGAGGACCGCGGAGTGACGTGGTCCGCTGAGTTGGA GGCAGATTTGCCCCGGTCTTGGCAACGGCATGGTGACCTAATGCTGCTTAGTGAAGACTGCTTCCAAGCC ACGCTGTGGAAAGGTCTGGAGCCAGAACTCTGGGAGACTGTTGCCTCGGCCCTGGGAGTTCAACGTTTGG CAAAACGAGGGCGGGTGCTACCTGATGGGACTCGAACTCCGTCAGTGACTCTGCTGCTGGGTGACCATGG CTGGGTAGAGCATATGGATAATGGCATCCGGTATAAGTTTGATGTGACACAGTGCATGTTCTCCTTTGGA AACATCACCGAGAAACTTCGAGTGGCGTCTCTGTCCTGTGCTGGAGAGGTGCTGGTGGATCTCTACGCAG GGATTGGTTATTTTACATTGCCCTTCCTAGTTCATGCTGGCGCTGCCTTCGTACATGCCTGTGAATGGAA TCCTCATGCTGTAGTTGCTTTAAGAAACAACTTGGAGATCAATGGAGTGGCAGACCGGTGCCAAATACAT TTTGGGGACAACAGGAAACTGAAGCTCTCAGACATTGCAGATAGGGTGAACCTCGGGCTCATTCCCAGTT CTAAAGAAGGCTGGCCCGTTGCCTGCCAAGTGCTTCGGAAGGACGTTGGGGGTATTCTGCATATCCACCA AAATGTGGAGTCTTTCTCAGGAAAGACTCCACAGCCACCTGGAAGCAATAACGTGGAAAAAGAGCACTGG CCTCGTCCCCAGAAAATCACCACTGATACACAGGGAAATGGAACTACTGAGAATTTCAGGGGGGAAATAT CATCGGCCAACAAACCAGAGTGGTGGAGATGGGCAGAATCTGCAGAAACTCAGATCGCCTCTCTTCTTCA TCAAGTGCATGGGAAACCATGGAGGACACGAATCTTGCATGTCCACCCAGTGAAATCTTACGCCCCTCAC GTGGATCACATAGTCCTAGATCTAGAATGCCGGCCCCTGACCTCTAGTTGGCCAGAAGGAGTAGATTTAC TGACACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203250 representing NM_026642
Red=Cloning site Green=Tags(s) MERECEESVVVAVVTEPRFTQRYRDYLEEQKLLDRLHRVAKLRDGAVALPVLAESLSEQHLQELRDRVAP GSTCVLTRLPDPLPSKKARVRSPAQILCLEVRRWVEDRGVTWSAELEADLPRSWQRHGDLMLLSEDCFQA TLWKGLEPELWETVASALGVQRLAKRGRVLPDGTRTPSVTLLLGDHGWVEHMDNGIRYKFDVTQCMFSFG NITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIH FGDNRKLKLSDIADRVNLGLIPSSKEGWPVACQVLRKDVGGILHIHQNVESFSGKTPQPPGSNNVEKEHW PRPQKITTDTQGNGTTENFRGEISSANKPEWWRWAESAETQIASLLHQVHGKPWRTRILHVHPVKSYAPH VDHIVLDLECRPLTSSWPEGVDLLTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026642 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026642.2, NP_080918.2 |
| RefSeq Size | 4133 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 68260 |
| UniProt ID | Q8BG71 |
| MW | 50.3 kDa |
| Gene Summary | S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203568 | Trmt12 (untagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12), (10ug) |
CNY 3,656.00 |
|
| MR203250L3 | Lenti ORF clone of Trmt12 (Myc-DDK-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12) |
CNY 5,230.00 |
|
| MR203250L4 | Lenti ORF clone of Trmt12 (mGFP-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12) |
CNY 5,230.00 |
