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Atp5f1 (NM_009725) Mouse Tagged ORF Clone
CAT＃: MR203291
- TrueORF®
Atp5f1 (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein
"NM_009725" in other vectors (3)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C76477 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203291 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCCGGGTGGTGCTTTCTGCTGCCGCCACAGCGGCCCCGTGTCTGAAGAACGCGGCCGCCCTAG GTCCAGGGGTATTACAGGCAACAAGGGCCTTTCACACAGGACAGCCTCGCCTTGCCCCTCTACCACCTCT TCCTGAATATGGAGGAAAAGTGCGTCTTGGGCTGATTCCTGAGGAATTTTTCCAGTTCCTTTACCCTAAG ACTGGTGTAACAGGACCTTATGTGCTTGGAACTGGACTTAGCTTGTATTTTCTATCCAAAGAAATATATG TGATTACCCCAGAGACCTTCTCTACCATATCAGTAGTAGGGTTGATAGTCTATGTGATTAAGAAATATGG CGCCTCTTTTGGAGAATTTATTGACAAACTTAATGAGGAAAAAATTGCTCAACTAGAAGAAGTAAAGCAG TCGAGCATGAAACAAATCCAGGATGCAATCGACATGGAGAAGGCACAGCAGGCACTGGTTCAGAAGCGCC ATTACCTCTTCGATGTGCAGAGGAATAACATTGCCCTGGCCTTGGAGGTCACTTACCGGGAACGGCTACA TAAAGCATATAAGGAGGTAAAGAATCGCCTGGACTACCACATCTCTGTACAGAACATGATGCGTCGCAAG GAGGAAGAACACATGATAGACTGGGTAGAAAAGCATGTGGTGAAGAGCATTTCTGTACAGCAGGAAAAGG AGACCATTGCCAAGTGCATTGAAGATCTAAAGCTGCTTGCAAAGAAGGCTCAAGCTCAGCCAATTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203291 protein sequence
Red=Cloning site Green=Tags(s) MLSRVVLSAAATAAPCLKNAAALGPGVLQATRAFHTGQPRLAPLPPLPEYGGKVRLGLIPEEFFQFLYPK TGVTGPYVLGTGLSLYFLSKEIYVITPETFSTISVVGLIVYVIKKYGASFGEFIDKLNEEKIAQLEEVKQ SSMKQIQDAIDMEKAQQALVQKRHYLFDVQRNNIALALEVTYRERLHKAYKEVKNRLDYHISVQNMMRRK EEEHMIDWVEKHVVKSISVQQEKETIAKCIEDLKLLAKKAQAQPIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009725 |
| ORF Size | 771 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009725.5 |
| RefSeq Size | 1486 bp |
| RefSeq ORF | 771 bp |
| Locus ID | 11950 |
| UniProt ID | Q9CQQ7 |
| MW | 28.9 kDa |
| Gene Summary | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208165 | Atp5f1 (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
| MR203291L3 | Lenti ORF clone of Atp5f1 (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR203291L4 | Lenti ORF clone of Atp5f1 (mGFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
