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Smug1 (NM_027885) Mouse Tagged Lenti ORF Clone
CAT＃: MR203761L4
- LentiORF®
Lenti ORF clone of Smug1 (mGFP-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1)
"NM_027885" in other vectors (4)
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CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 1200013B09Rik; A930006H09Rik; C85220 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR203761).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027885 |
| ORF Size | 840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027885.3, NP_082161.2 |
| RefSeq Size | 3611 bp |
| RefSeq ORF | 840 bp |
| Locus ID | 71726 |
| UniProt ID | Q6P5C5 |
| Gene Summary | Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205251 | Smug1 (untagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1), (10ug) |
CNY 2,400.00 |
|
| MG203761 | Smug1 (tGFP-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 2,850.00 |
|
| MR203761 | Smug1 (Myc-DDK-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 2,400.00 |
|
| MR203761L3 | Lenti ORF clone of Smug1 (Myc-DDK-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 4,750.00 |
