Marchf8 (NM_027920) Mouse Tagged ORF Clone
CAT#: MR203891
- TrueORF®
41341 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8)
ORF Plasmid: tGFP
"NM_027920" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1300017E09Rik; M; Marc; MARCH-VIII; March8; Mir |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203891 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGCCATTGCACCAGATCTCTGCCATCCCTTCTCAGGATGCCATTTCTGCTAGAGTCTACAGAA GCAAGACCAAAGATAAGGAGCAGAATGAGAAGACTTTGGGACATTCCATGAGTCATCCAAGCAACATTTC TAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTGCCTTCTCTCGCACTTCTGTCACACCA TCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGACGAGAGCCCTCTGATCACCCCCTGTC ACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAGCAGTGGATCAAGAGTTCTGACACACG CTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGCTGAAACCTTTGAGGAAATGGGAGAAG TTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGTGACCTTCCATGTCATTGCTATCACCT GTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAGGAAATCAAGCAGGGTCAGGTAACAGG AATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCATCGGCTTCACTGGAGGACTTCTCTTT ATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACTCAAGGCTTACAATAGAGTGATCTATG TTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAGTCTGCACTTACAGAGCCCACCCTTGA AAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTTCTTGCACAGAGCCTGAAGACACTGGA GCAGAAATTATTAACGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203891 protein sequence
Red=Cloning site Green=Tags(s) MSMPLHQISAIPSQDAISARVYRSKTKDKEQNEKTLGHSMSHPSNISKAGSSPPSTTAPVSAFSRTSVTP SNQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIMETKLKPLRKWEK LQMTASERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGQVTGILEWPFWTKLVVVAIGFTGGLLF MYVQCKVYLQLWKRLKAYNRVIYVQNCPETSKKNIFEKSALTEPTLENKEGHGMCHSTTNSSCTEPEDTG AEIINV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027920 |
ORF Size | 861 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027920.5 |
RefSeq Size | 4521 bp |
RefSeq ORF | 861 bp |
Locus ID | 71779 |
UniProt ID | Q9DBD2 |
MW | 32.2 kDa |
Gene Summary | The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203594 | 41341 (untagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8), (10ug) |
CNY 2,400.00 |
|
MG203891 | 41341 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8) |
CNY 4,000.00 |
|
MR203891L3 | Lenti ORF clone of 41341 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8) |
CNY 4,750.00 |
|
MR203891L4 | Lenti ORF clone of 41341 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8) |
CNY 4,750.00 |
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