Snrpa (NM_015782) Mouse Tagged ORF Clone
CAT#: MR203911
- TrueORF®
Snrpa (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein polypeptide A (Snrpa), transcript variant 1
"NM_015782" in other vectors (2)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C430021M15Rik; Rnu1a-1; Rnu1a1; U1-A; U1A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203911 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCATAGCCACCATGCCAGTTCCCGAGACCCGTGCCAACCACACTATTTATATCAACAATCTCA ATGAGAAGATCAAGAAGGATGAGCTCAAGAAGTCCCTGTATGCCATCTTCTCCCAGTTTGGCCAGATCCT GGATATCCTGGTGTCTCGGATCATGAAGATGAGGGGCCAGGCCTTCGTCATCTTCAAGGAGGTCACCAGC GCCACCAATGCCCTGCGCTCCATGCAGGGCTTCCCTTTCTACGACAAGCCCATGCGCATCCAGTACGCAA AGACTGACTCGGACATCATTGCCAAGATGAAGGGCACCTATGTGGAGAGAGATCGCAAACGAGAGAAGAG GAAGCCCAAGAGTCAGGAGACACCTGCTGCCAAAAAGGCTGTTCAGGGTGGGGCAGCTGCGCCCGTGGTG GGGGCTGTCCAGCCCGTACCGGGCATGCCACCGATGCCTCAGGCACCCCGCATCATGCACCATATGCCAG GACAGCCTCCCTACATGCCGCCACCTGGCATGATCCCGCCACCAGGCCTCGCTCCTGGCCAGATCCCCCC TGGGGCCATGCCCCCACAGCAGCTCATGCCTGGGCAGATGCCGCCTGCCCAGCCTCTCTCCGAGAACCCA CCCAATCACATCCTGTTCCTCACCAACCTGCCTGAGGAGACCAACGAGCTCATGCTCTCCATGCTCTTCA ACCAGTTCCCTGGCTTCAAGGAGGTGCGTCTGGTCCCTGGGCGCCATGACATCGCCTTCGTGGAGTTTGA CAATGAAGTGCAGGCTGGGGCAGCACGAGATGCCCTGCAAGGCTTTAAGATCACACAAAACAATGCTATG AAGATCTCTTTTGCCAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203911 protein sequence
Red=Cloning site Green=Tags(s) MATIATMPVPETRANHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRIMKMRGQAFVIFKEVTS ATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTYVERDRKREKRKPKSQETPAAKKAVQGGAAAPVV GAVQPVPGMPPMPQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENP PNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAM KISFAKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015782 |
ORF Size | 864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015782.3, NP_056597.3 |
RefSeq Size | 1382 bp |
RefSeq ORF | 864 bp |
Locus ID | 53607 |
UniProt ID | Q62189 |
MW | 31.8 kDa |
Gene Summary | Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR203911L3 | Lenti ORF clone of Snrpa (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein polypeptide A (Snrpa), transcript variant 1 |
CNY 4,750.00 |
|
MR203911L4 | Lenti ORF clone of Snrpa (mGFP-tagged) - Mouse small nuclear ribonucleoprotein polypeptide A (Snrpa), transcript variant 1 |
CNY 4,750.00 |