Vta1 (NM_025418) Mouse Tagged ORF Clone
CAT#: MR204382
- TrueORF®
Vta1 (Myc-DDK-tagged) - Mouse Vps20-associated 1 homolog (S. cerevisiae) (Vta1)
ORF Plasmid: tGFP
"NM_025418" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110001D18Rik; 1110059P08Rik; AU040813; C85340; LIP5; SBP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204382 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGCTGGCCCCTCTGCCGCCGCTGCCCGCGCAGTTTAAGAGCATACAGCACCATTTGAGAACGG CGCAGGAGCATGACAAGCGGGACCCCGTGGTGGCCTACTACTGTCGTTTATATGCTATGCAAACTGGAAT GAAGATTGATAGTAAAACTCCTGAGTGTCGTAAATTTTTATCAAAGCTAATGGATCAGTTAGAAGCTCTT AAAAAACAGTTGGGGGATAATGAAGCTGTTACTCAAGAAATAGTTGGTTGTGCCCATTTGGAAAATTATG CTTTGAAAATGTTCTTGTATGCAGACAATGAAGATCGAGCTGGACGATTTCACAAAAACATGATCAAGTC CTTCTACACTGCCAGCCTTTTAATAGATGTCATCACAGTGTTTGGTGAACTCACAGATGAAAATGTGAAA CATAGAAAGTATGCAAGGTGGAAGGCAACATATATTCATAACTGCTTAAAGAATGGGGAGACTCCTCAAG CAGGCCCTGTTGGAATAGAGGAAGAGAATGATGTTGAAGAAAATGAAGATGTTGGCGCAACCTCTCTGCC CACTCAGCCACCTCAGCCATCATCATCTTCAGCATACGACCCAAGCAACTTGGCACCAGGCAGCTACAGT GGCATACAGATTCCTCCAGGTGCACATGCTCCAGCTAATACCCCTGCAGAAGTGCCACACAGCACAGGTG TAACGAGTAATGCCGTCCAGCCTAGTCCACAGACTGTCCCAGCTGCTCCAGCTGTTGATCCTGACCTGTA CACAGCTTCCCAGGGGGATATCCGCCTGACCCCGGAGGACTTCGCTAGAGCTCAGAAGTACTGCAAGTAT GCTGGCAGTGCCCTGCAGTATGAAGATGTGGGCACCGCTGTGCAGAACCTCCAGAAGGCCCTCAGGCTGC TGACCACAGGCAGGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204382 protein sequence
Red=Cloning site Green=Tags(s) MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFLSKLMDQLEAL KKQLGDNEAVTQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLIDVITVFGELTDENVK HRKYARWKATYIHNCLKNGETPQAGPVGIEEENDVEENEDVGATSLPTQPPQPSSSSAYDPSNLAPGSYS GIQIPPGAHAPANTPAEVPHSTGVTSNAVQPSPQTVPAAPAVDPDLYTASQGDIRLTPEDFARAQKYCKY AGSALQYEDVGTAVQNLQKALRLLTTGRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025418 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025418.3, NP_079694.2 |
RefSeq Size | 1343 bp |
RefSeq ORF | 930 bp |
Locus ID | 66201 |
UniProt ID | Q9CR26 |
MW | 33.9 kDa |
Gene Summary | Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies (By similarity). Involved in the sorting and down-regulation of EGFR.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202859 | Vta1 (untagged) - Mouse Vps20-associated 1 homolog (S. cerevisiae) (Vta1), (10ug) |
CNY 2,400.00 |
|
MG204382 | Vta1 (tGFP-tagged) - Mouse RIKEN cDNA 1110059P08 gene (1110059P08Rik) |
CNY 2,850.00 |
|
MR204382L3 | Lenti ORF clone of Vta1 (Myc-DDK-tagged) - Mouse Vps20-associated 1 homolog (S. cerevisiae) (Vta1) |
CNY 4,750.00 |
|
MR204382L4 | Lenti ORF clone of Vta1 (mGFP-tagged) - Mouse Vps20-associated 1 homolog (S. cerevisiae) (Vta1) |
CNY 4,750.00 |