Pycr1 (NM_144795) Mouse Tagged ORF Clone
CAT#: MR204385
- TrueORF®
Pycr1 (Myc-DDK-tagged) - Mouse pyrroline-5-carboxylate reductase 1 (Pycr1)
ORF Plasmid: tGFP
"NM_144795" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | P5CR 1; P5CR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204385 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTAGGCTTCATCGGGGCAGGGCAGCTAGCCTTTGCTCTAGCCAAGGGCTTCACAGCTGCAGGTG TTCTGGCTGCACACAAGATAATGGCCAGCTCCCCAGACATGGACCAAGCTACGGTCTCGGCCCTCCGGAA GATAGGGGTGAACCTGACACCCCACAACAAAGAGACTGTGCGCCACAGTGATGTGCTCTTCCTGGCTGTG AAGCCACACATCATCCCCTTCATCCTGGATGAAATTGGGGCGAACATTGAGGACAGGCACATTGTGGTGT CTTGTGCGGCAGGGGTCACCATCAACTCCATCGAAAAGAAGCTGACAGCGTTCCAACCTGCCCCCAAAGT CATCCGATGTATGACTAACACCCCGGTCGTGGTACGGGAGGGGGTCACTGTGTATGCCACAGGTACTCAC GCTCAGGTGGAAGATGGCAGGCTTGTGGAGCAGCTCATGGGCAGCGTGGGCTTCTGCACAGAGGTGGAGG AAGATCTGATCGATGCTGTCACAGGGCTCAGTGGCAGCGGCCCTGCCTATGCTTTCACAGCCCTGGATGC TCTGGCTGACGGTGGTGTGAAAATGGGGCTTCCCAGACGCCTGGCAGTCCGCCTCGGGGCCCAGGCCCTC CTGGGGGCAGCCAAGATGCTACTAGACTCAGAACAGCATCCCAGCCAGCTCAAGGATAATGTCTGCTCTC CGGGTGGGGCCACCATCCATGCCTTGCATGTGCTGGAGAGCGGGGGCTTTCGCTCCTTGCTTATCAACGC TGTGGAAGCCTCCTGCATCCGTACACGGGAGCTGCAGACCATGGCTGACCAGGAGACCATCTCTCCTGCT GCCATAAAGAAGACTGTACTGGATAAGGTGAAGCTGGACTCCTCTGCTGGGGCCTCTCTGTCTTCTGACC ATGTCAAGCCACTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204385 protein sequence
Red=Cloning site Green=Tags(s) MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDQATVSALRKIGVNLTPHNKETVRHSDVLFLAV KPHIIPFILDEIGANIEDRHIVVSCAAGVTINSIEKKLTAFQPAPKVIRCMTNTPVVVREGVTVYATGTH AQVEDGRLVEQLMGSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQAL LGAAKMLLDSEQHPSQLKDNVCSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQTMADQETISPA AIKKTVLDKVKLDSSAGASLSSDHVKPLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144795 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144795.3 |
RefSeq Size | 2914 bp |
RefSeq ORF | 930 bp |
Locus ID | 209027 |
UniProt ID | Q922W5 |
MW | 32.4 kDa |
Gene Summary | Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200392 | Pycr1 (untagged) - Mouse pyrroline-5-carboxylate reductase 1 (Pycr1), (10ug) |
CNY 2,400.00 |
|
MG204385 | Pycr1 (tGFP-tagged) - Mouse pyrroline-5-carboxylate reductase 1 (Pycr1) |
CNY 2,850.00 |
|
MR204385L3 | Lenti ORF clone of Pycr1 (Myc-DDK-tagged) - Mouse pyrroline-5-carboxylate reductase 1 (Pycr1) |
CNY 4,750.00 |
|
MR204385L4 | Lenti ORF clone of Pycr1 (mGFP-tagged) - Mouse pyrroline-5-carboxylate reductase 1 (Pycr1) |
CNY 4,750.00 |