Sucnr1 (NM_032400) Mouse Tagged ORF Clone
CAT#: MR204545
- TrueORF®
Sucnr1 (Myc-DDK-tagged) - Mouse succinate receptor 1 (Sucnr1)
ORF Plasmid: tGFP
"NM_032400" in other vectors (4)
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CNY 3,600.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Gpr91 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204545 representing NM_032400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGAATTTATCTTGTGAGAATTGGTTGGCAACAGAGGCTATCTTGAATAAGTACTACCTCTCTG CATTTTATGCAATCGAGTTCATTTTTGGACTGCTTGGGAATGTCACTGTGGTGTTCGGCTACCTCTTCTG CATGAAGAACTGGAACAGCAGCAATGTCTATCTTTTTAACCTTTCCATCTCTGACTTTGCTTTCCTGTGC ACCCTTCCCATCCTGATAAAGAGTTATGCCAATGATAAGGGGACCTATGGAGATGTTCTCTGTATAAGCA ACCGATATGTGCTTCACACCAACCTCTACACCAGCATCCTCTTCCTCACTTTCATTAGCATGGACCGATA TCTGCTCATGAAGTACCCTTTCCGAGAACACTTTCTACAAAAGAAGGAATTTGCCATTTTAATCTCGCTG GCTGTCTGGGCCTTAGTGACCTTAGAAGTTCTACCCATGCTCACTTTCATCAATTCTGTCCCAAAAGAAG AGGGCAGTAACTGCATCGACTATGCAAGTTCTGGAAACCCTGAACACAATCTCATTTACAGCCTCTGCCT GACTTTGTTGGGCTTCCTAATTCCTCTCTCTGTGATGTGCTTCTTCTACTACAAGATGGTAGTCTTCTTA AAGAGGAGGAGCCAGCAGCAAGCAACTGCCCTGCCACTGGACAAACCCCAACGCCTGGTGGTCCTGGCGG TTGTGATCTTCTCTATACTCTTCACACCCTATCATATCATGCGCAATTTGAGGATCGCCTCACGCCTGGA TAGTTGGCCACAAGGATGTACACAGAAGGCCATCAAATCTATATACACACTGACACGGCCTCTGGCCTTT CTGAACAGTGCCATCAATCCCATCTTCTACTTCCTCATGGGAGACCATTACAGAGAGATGCTGATTAGTA AGTTCAGACAATACTTCAAGTCCCTTACATCCTTCAGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204545 representing NM_032400
Red=Cloning site Green=Tags(s) MAQNLSCENWLATEAILNKYYLSAFYAIEFIFGLLGNVTVVFGYLFCMKNWNSSNVYLFNLSISDFAFLC TLPILIKSYANDKGTYGDVLCISNRYVLHTNLYTSILFLTFISMDRYLLMKYPFREHFLQKKEFAILISL AVWALVTLEVLPMLTFINSVPKEEGSNCIDYASSGNPEHNLIYSLCLTLLGFLIPLSVMCFFYYKMVVFL KRRSQQQATALPLDKPQRLVVLAVVIFSILFTPYHIMRNLRIASRLDSWPQGCTQKAIKSIYTLTRPLAF LNSAINPIFYFLMGDHYREMLISKFRQYFKSLTSFRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032400 |
ORF Size | 951 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032400.1, NM_032400.2, NP_115776.1, NP_115776.2 |
RefSeq Size | 1598 bp |
RefSeq ORF | 954 bp |
Locus ID | 84112 |
UniProt ID | Q99MT6 |
MW | 37.2 kDa |
Gene Summary | Receptor for succinate.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling
,Trauelsen, M;Hiron, TK;Lin, D;Petersen, JE;Breton, B;Husted, AS;Hjorth, SA;Inoue, A;Frimurer, TM;Bouvier, M;O'Callaghan, CA;Schwartz, TW;,
Cell reports
,PubMed ID 34133934
[SUCNR1]
|
Structure-Activity Investigations and Optimisations of Non-metabolite Agonists for the Succinate Receptor 1
,Rexen Ulven, E;Trauelsen, M;Brvar, M;Lückmann, M;Bielefeldt, LØ;Jensen, LKI;Schwartz, TW;Frimurer, TM;,
Sci Rep
,PubMed ID 29968758
[SUCNR1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211973 | Sucnr1 (untagged) - Mouse succinate receptor 1 (Sucnr1), (10ug) |
CNY 3,600.00 |
|
MG204545 | Sucnr1 (tGFP-tagged) - Mouse succinate receptor 1 (Sucnr1) |
CNY 5,200.00 |
|
MR204545L3 | Lenti ORF clone of Sucnr1 (Myc-DDK-tagged) - Mouse succinate receptor 1 (Sucnr1) |
CNY 5,890.00 |
|
MR204545L4 | Lenti ORF clone of Sucnr1 (mGFP-tagged) - Mouse succinate receptor 1 (Sucnr1) |
CNY 6,000.00 |