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Riok1 (BC002158) Mouse Tagged ORF Clone
CAT＃: MR204785
- TrueORF®
Riok1 (Myc-DDK-tagged) - Mouse RIO kinase 1 (yeast) (cDNA clone MGC:7300 IMAGE:3485522)
ORF Plasmid: tGFP
"BC002158" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3110046C13Rik, Ad034, MGC7300 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204785 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGGACGTGGGCAGAGAAGGAGATGAGGAATTTGTGCAGGCTAAAAACAGCAAACATACCATGTC CAGAACCAATCAGGCTAAGAAGTCATGTTCTTCTCATGGGCTTCATTGGCAAGGATGACATGCCAGCCCC ACTTTTGAAAAATGTCCAGCTGTCAGAGTCCAAGGCACGGGAGTTGTACCTGCAGGTCATTCAGTACATG AGGAAAATGTATCAGGATGCTAGACTTGTCCACGCGGATCTCAGTGAATTCAACATGCTGTACCATGGTG GAGATGTTTACATCATTGATGTTTCTCAGTCTGTGGAGCATGACCACCCACATGCATTGGAGTTCTTGAG AAAAGACTGTACCAATGTCAATGATTTCTTTTCCAAGCATGCTGTTGCAGTGATGACCGTGCGGGAGCTC TTCGACTTCGTCACAGATCCCTCCATCACTGCTGACAACATGGATGCTTACCTGGAAAAGGCTATGGAAA TAGCATCCCAGAGGACCAAGGAAGAAAAGACTAGCCAAGATCATGTGGATGAAGAGGTGTTCAAACAAGC ATATATTCCCAGAACCTTAAACGAAGTAAAGAATTATGAGAGAGATGTGGACATCATGATGAGGTTAAAG GAAGAAGACATGGCTTTGAACACTCAGCAAGACAACATTCTATACCAGACTGTCATGGGATTGAAAAAAG ATTTGTCAGGAGTCCAGAAGGTCCCCGCGCTCCTAGAAAGTGAAGTTAAGGAAGAGACTTGTTTTGGTTC AGACGATGCTGGGGGCTCTGAGTGCTCCGACACAGTCTCTGAAGAGCAGGAAGATCAAGCCGGATGCAGA AACCATATTGCTGACCCCGACGTTGATAAAAAGGAAAGAAAAAAGATGGTCAAGGAAGCCCAGAGAGAGA AGAGAAAAAATAAAATCCCTAAACATGTGAAAAAAAGAAAGGAAAAAACAGCCAAGGCCAAAAAAGGCAA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204785 protein sequence
Red=Cloning site Green=Tags(s) MVRTWAEKEMRNLCRLKTANIPCPEPIRLRSHVLLMGFIGKDDMPAPLLKNVQLSESKARELYLQVIQYM RKMYQDARLVHADLSEFNMLYHGGDVYIIDVSQSVEHDHPHALEFLRKDCTNVNDFFSKHAVAVMTVREL FDFVTDPSITADNMDAYLEKAMEIASQRTKEEKTSQDHVDEEVFKQAYIPRTLNEVKNYERDVDIMMRLK EEDMALNTQQDNILYQTVMGLKKDLSGVQKVPALLESEVKEETCFGSDDAGGSECSDTVSEEQEDQAGCR NHIADPDVDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKAKKGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC002158 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002158, AAH02158 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 983 bp |
| Locus ID | 71340 |
| MW | 37.8 kDa |
| Gene Summary | This gene encodes a member of the RIO family of atypical serine protein kinases. A similar protein in humans is a component of the protein arginine methyltransferase 5 complex that specifically recruits the RNA-binding protein nucleolin as a methylation substrate. [provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200302 | Riok1 (untagged) - Mouse RIO kinase 1 (yeast) (cDNA clone MGC:7300 IMAGE:3485522), (10ug) |
CNY 2,400.00 |
|
| MG204785 | Riok1 (tGFP-tagged) - Mouse RIO kinase 1 (yeast) (cDNA clone MGC:7300 IMAGE:3485522) |
CNY 2,850.00 |
|
| MR204785L3 | Lenti ORF clone of Riok1 (Myc-DDK-tagged) - Mouse RIO kinase 1 (yeast) (cDNA clone MGC:7300 IMAGE:3485522) |
CNY 4,750.00 |
|
| MR204785L4 | Lenti ORF clone of Riok1 (mGFP-tagged) - Mouse RIO kinase 1 (yeast) (cDNA clone MGC:7300 IMAGE:3485522) |
CNY 4,750.00 |
