Fdps (NM_134469) Mouse Tagged ORF Clone

CAT#: MR205363

  • TrueORF®

Fdps (Myc-DDK-tagged) - Mouse farnesyl diphosphate synthetase (Fdps)

ORF Plasmid: DDK tGFP



  "NM_134469" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 6030492I17Rik; AI256750; Fdpsl1; mKIAA1293
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205363 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGGAACCAGAAATTGGATGCTTATAACCAAGAAAAGCAGAATTTCATCCAGCACTTCTCCCAGA
TCGTCAAGGTGCTGACTGAGAAGGAGCTGGGACACCCAGAGATAGGGGATGCTATTGCCCGGCTCAAGGA
GGTCCTAGAGTACAATGCCTTAGGAGGCAAGTACAACCGGGGTTTGACCGTGGTACAAGCCTTCCAGGAG
CTGGTGGAGCCGAAGAAACAGGATGCTGAGAGTCTTCAGCGGGCCCTGACAGTGGGCTGGTGTGTAGAAC
TGCTCCAGGCTTTCTTCCTTGTGTCAGATGACATCATGGACTCTTCCCTCACTCGCCGGGGACAGATCTG
CTGGTATCAGAAGCCAGGCATAGGCTTGGATGCCATCAACGACGCTCTGCTTCTGGAAGCCTCCATCTAT
CGTTTGCTGAAGTTCTACTGCAGGGAGCAGCCCTACTACCTGAACCTGCTGGAGCTCTTTCTGCAGAGTT
CCTATCAGACAGAGATCGGGCAGACTCTAGACCTCATGACAGCACCCCAGGGCCATGTGGATCTTGGTAG
ATACACTGAAAAGAGGTACAAATCGATTGTCAAGTACAAGACGGCTTTCTACTCTTTCTACCTGCCTATT
GCGGCCGCCATGTACATGGCAGGCATTGATGGGGAGAAGGAACACGCCAATGCCCTGAAGATCCTGATGG
AGATGGGCGAGTTCTTCCAGGTCCAGGACGACTACCTTGATCTCTTTGGAGACCCCAGTGTGACGGGAAA
GGTCGGCACTGACATCCAGGACAACAAATGCAGCTGGCTGGTGGTTCAGTGTCTGCTACGAGCCTCTCCT
CAACAGCGCCAGATCTTAGAGGAGAATTATGGGCAGAAGGACCCAGAAAAAGTGGCTCGGGTGAAAGCAC
TGTATGAGGCGCTGGATCTGCAGTCTGCTTTCTTCAAGTATGAGGAAGACAGTTACAACCGCCTCAAGAG
TCTCATAGAGCAGTGCTCTGCGCCCCTGCCCCCATCCATCTTCATGGAACTTGCAAACAAGATCTACAAA
CGGAGAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205363 protein sequence
Red=Cloning site Green=Tags(s)

MNGNQKLDAYNQEKQNFIQHFSQIVKVLTEKELGHPEIGDAIARLKEVLEYNALGGKYNRGLTVVQAFQE
LVEPKKQDAESLQRALTVGWCVELLQAFFLVSDDIMDSSLTRRGQICWYQKPGIGLDAINDALLLEASIY
RLLKFYCREQPYYLNLLELFLQSSYQTEIGQTLDLMTAPQGHVDLGRYTEKRYKSIVKYKTAFYSFYLPI
AAAMYMAGIDGEKEHANALKILMEMGEFFQVQDDYLDLFGDPSVTGKVGTDIQDNKCSWLVVQCLLRASP
QQRQILEENYGQKDPEKVARVKALYEALDLQSAFFKYEEDSYNRLKSLIEQCSAPLPPSIFMELANKIYK
RRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134469
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_134469.4, NP_608219.1
RefSeq Size 1262 bp
RefSeq ORF 1062 bp
Locus ID 110196
UniProt ID Q920E5
MW 40.6 kDa
Gene Summary Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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