Fos (NM_010234) Mouse Tagged ORF Clone
CAT#: MR205933
- TrueORF®
Fos (Myc-DDK-tagged) - Mouse FBJ osteosarcoma oncogene (Fos)
ORF Plasmid: tGFP
"NM_010234" in other vectors (4)
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CNY 5,488.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | c-fos; cFos; D12Rfj1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205933 representing NM_010234
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTTCTCGGGTTTCAACGCCGACTACGAGGCGTCATCCTCCCGCTGCAGTAGCGCCTCCCCGGCCG GGGACAGCCTTTCCTACTACCATTCCCCAGCCGACTCCTTCTCCAGCATGGGCTCTCCTGTCAACACACA GGACTTTTGCGCAGATCTGTCCGTCTCTAGTGCCAACTTTATCCCCACGGTGACAGCCATCTCCACCAGC CCAGACCTGCAGTGGCTGGTGCAGCCCACTCTGGTCTCCTCCGTGGCCCCATCGCAGACCAGAGCGCCCC ATCCTTACGGACTCCCCACCCAGTCTGCTGGGGCTTACGCCAGAGCGGGAATGGTGAAGACCGTGTCAGG AGGCAGAGCGCAGAGCATCGGCAGAAGGGGCAAAGTAGAGCAGCTATCTCCTGAAGAGGAAGAGAAACGG AGAATCCGAAGGGAACGGAATAAGATGGCTGCAGCCAAGTGCCGGAATCGGAGGAGGGAGCTGACAGATA CACTCCAAGCGGAGACAGATCAACTTGAAGATGAGAAGTCTGCGTTGCAGACTGAGATTGCCAATCTGCT GAAAGAGAAGGAAAAACTGGAGTTTATTTTGGCAGCCCACCGACCTGCCTGCAAGATCCCCGATGACCTT GGCTTCCCAGAGGAGATGTCTGTGGCCTCCCTGGATTTGACTGGAGGTCTGCCTGAGGCTTCCACCCCAG AGTCTGAGGAGGCCTTCACCCTGCCCCTTCTCAACGACCCTGAGCCCAAGCCATCCTTGGAGCCAGTCAA GAGCATCAGCAACGTGGAGCTGAAGGCAGAACCCTTTGATGACTTCTTGTTTCCGGCATCATCTAGGCCC AGTGGCTCAGAGACCTCCCGCTCTGTGCCAGATGTGGACCTGTCCGGTTCCTTCTATGCAGCAGACTGGG AGCCTCTGCACAGCAATTCCTTGGGGATGGGGCCCATGGTCACAGAGCTGGAGCCCCTGTGTACTCCCGT GGTCACCTGTACTCCGGGCTGCACTACTTACACGTCTTCCTTTGTCTTCACCTACCCTGAAGCTGACTCC TTCCCAAGCTGTGCCGCTGCCCACCGAAAGGGCAGCAGCAGCAACGAGCCCTCCTCCGACTCCCTGAGCT CACCCACGCTGCTGGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205933 representing NM_010234
Red=Cloning site Green=Tags(s) MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNTQDFCADLSVSSANFIPTVTAISTS PDLQWLVQPTLVSSVAPSQTRAPHPYGLPTQSAGAYARAGMVKTVSGGRAQSIGRRGKVEQLSPEEEEKR RIRRERNKMAAAKCRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKLEFILAAHRPACKIPDDL GFPEEMSVASLDLTGGLPEASTPESEEAFTLPLLNDPEPKPSLEPVKSISNVELKAEPFDDFLFPASSRP SGSETSRSVPDVDLSGSFYAADWEPLHSNSLGMGPMVTELEPLCTPVVTCTPGCTTYTSSFVFTYPEADS FPSCAAAHRKGSSSNEPSSDSLSSPTLLAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010234 |
ORF Size | 1140 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010234.3 |
RefSeq Size | 2107 bp |
RefSeq ORF | 1143 bp |
Locus ID | 14281 |
UniProt ID | P01101 |
MW | 41.3 kDa |
Gene Summary | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling (By similarity). Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
c-FOS drives reversible basal to squamous cell carcinoma transition
,null,
Cell reports
,PubMed ID 34610301
[Fos]
|
Involvement of c-Fos in the Promotion of Cancer Stem-like Cell Properties in Head and Neck Squamous Cell Carcinoma
,Muhammad, N;Bhattacharya, S;Steele, R;Phillips, N;Ray, RB;,
Clin. Cancer Res.
,PubMed ID 27965308
[FOS]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203181 | Fos (untagged) - Mouse FBJ osteosarcoma oncogene (Fos), (10ug) |
CNY 3,656.00 |
|
MG205933 | Fos (tGFP-tagged) - Mouse FBJ osteosarcoma oncogene (Fos) |
CNY 7,088.00 |
|
MR205933L3 | Lenti ORF clone of Fos (Myc-DDK-tagged) - Mouse FBJ osteosarcoma oncogene (Fos) |
CNY 7,888.00 |
|
MR205933L4 | Lenti ORF clone of Fos (mGFP-tagged) - Mouse FBJ osteosarcoma oncogene (Fos) |
CNY 7,888.00 |