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Dmtn (NM_013514) Mouse Tagged ORF Clone
CAT＃: MR205979
- TrueORF®
Epb4.9 (Myc-DDK-tagged) - Mouse erythrocyte protein band 4.9 (Epb4.9)
ORF Plasmid: tGFP
"NM_013514" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI325486; dematin; Epb4.9; Epb49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205979 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACGTCTGCAGAAGCAACCTCTTACCTCCCCGGGGAGCGTCAGCTCCTCCAGAGACTCCAGTGTGC CCGGCTCTCCCTCCAGCATCGTGGCCAAGATGGACAACCAGGTGTTGGGCTACAAGGATCTGGCTGCCAT CCCCAAGGACAAGGCCATCCTGGACATTGAGCGACCTGACCTCATGATCTATGAGCCCCACTTTACCTAT TCCCTCCTGGAACATGTAGAGCTGCCCAGAAGCCGGGAGTGCTCACTGTCACCCAAATCCACATCCCCCC CACCGTCTCCAGAGGTGTGGGCGGAGAGCCGGACTCTTGGAATCATCTCTCAGGCTTCAACCCCAAGGAC CACAGGGACCCCCAGGACCAGCCTGCCCCACTTCCACCACCCTGAGACTACCCGCCCGGATTCCAACATC TACAAGAAGCCACCCATCTACAAACAGAGAGAATCCGTGGGAGGCAGCCCTCAGAGCAAGCACCTCATCG AGGACCTCATCATCGAATCCTCCAAGTTTCCTGCAGCGCAGCCCCCTGACCCCAACCAGCCAGCCAAGAT AGAGACTGACTACTGGCCATGTCCCCCGTCGCTGGCCGTTGTGGAGACAGAATGGAGGAAACGGAAGGCA TCTCGAAAGGGGGCAGAGGAAGAGGAGGAAGAGGAAGACGATGACTCTGAAGAGGAGATTAAGGCCATCA GGGAACGGCAGAAAGAGGAGCTCAGTAAGGTTACTTCCAACTTGGGAAAGATGATCTTGAAAGAAGAGAT GGAAAAGTCATTGCCCATCCGGAGGAAAACACGCTCTCTGCCTGACCGGACACCCTTCCATACCTCCTTG CATTCGGGAACATCTAAATCCTCTTCGCTTCCTTCCTATGGCAGGACCACCCTGAGCCGGCTACAGTCCA CAGAATTCAGCCCATCGGGAAGTGAGGCTGGGAGCCCAGGCCTGCAGATCTATCCCTATGAGATGCTGGT GGTGACCAATAAGGGGAGAACTAAGCTGCCTCCGGGTGTGGACCGCATGAGGCTTGAGAGGCATTTGTCA GCAGAGGACTTCTCTAGGGTCTTCGCCATGTCTCCCGAGGAGTTTGGCAAGCTGGCCCTGTGGAAGCGGA ACGAACTTAAGAAGAAAGCTTCCCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205979 protein sequence
Red=Cloning site Green=Tags(s) MERLQKQPLTSPGSVSSSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTY SLLEHVELPRSRECSLSPKSTSPPPSPEVWAESRTLGIISQASTPRTTGTPRTSLPHFHHPETTRPDSNI YKKPPIYKQRESVGGSPQSKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKA SRKGAEEEEEEEDDDSEEEIKAIRERQKEELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSL HSGTSKSSSLPSYGRTTLSRLQSTEFSPSGSEAGSPGLQIYPYEMLVVTNKGRTKLPPGVDRMRLERHLS AEDFSRVFAMSPEEFGKLALWKRNELKKKASLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013514 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013514.4, NP_038542.1 |
| RefSeq Size | 4129 bp |
| RefSeq ORF | 1152 bp |
| Locus ID | 13829 |
| UniProt ID | Q9WV69 |
| MW | 43 kDa |
| Gene Summary | Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208441 | Dmtn (untagged) - Mouse erythrocyte protein band 4.9 (Epb4.9), (10ug) |
CNY 3,990.00 |
|
| MG205979 | Epb4.9 (tGFP-tagged) - Mouse erythrocyte protein band 4.9 (Epb4.9) |
CNY 3,710.00 |
|
| MR205979L3 | Lenti ORF clone of Epb4.9 (Myc-DDK-tagged) - Mouse erythrocyte protein band 4.9 (Epb4.9) |
CNY 5,230.00 |
|
| MR205979L4 | Lenti ORF clone of Epb4.9 (mGFP-tagged) - Mouse erythrocyte protein band 4.9 (Epb4.9) |
CNY 5,230.00 |
