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Evl (NM_007965) Mouse Tagged ORF Clone
CAT＃: MR206142
- TrueORF®
Evl (Myc-DDK-tagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl), transcript variant 2
"NM_007965" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI528774; b2b2600Clo |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206142 representing NM_007965
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAACAGAGTATCTGCCAAGCGCGGGCCTCCGTGATGGTCTACGATGACACCAGTAAGAAGTGGG TACCGATCAAGCCTGGCCAGCAGGGATTCAGCCGGATCAACATCTACCACAACACTGCCAGCAGCACCTT CAGAGTGGTCGGGGTCAAGCTACAGGACCAGCAGGTTGTGATCAATTATTCAATTGTTAAAGGGCTGAAG TACAATCAGGCAACACCCACCTTCCATCAGTGGCGAGATGCCCGTCAGGTCTATGGCTTAAACTTTGCAA GTAAGGAAGAAGCAACCACATTCTCCAATGCCATGCTCTTTGCCCTGAACATCATGAATTCCCAAGAAGG AGGCCCCTCCACACAGCGTCAGGTGCAGAATGGCCCCTCTCCTGAGGAGATGGACATCCAGAGAAGACAA GTAATGGAGCAGCAGCACCGCCAGGAGTCTCTGGAGAGGAGAATCTCGGCCACAGGGCCCATTCTCCCCC CTGGGCATCCCTCATCGGCAGCCAGCACCACTCTCTCCTGTAGTGGACCTCCACCCCCGCCTCCACCCCC AGTTCCACCTCCACCCACAGGGTCTACTCCCCCACCCCCACCCCCACTGCCAGCTGGAGGAGCCCAGGGG ACCAACCATGATGAGAGCTCTGCATCAGGACTGGCTGCTGCTCTGGCGGGAGCCAAGCTAAGGAGGGTGC AGCGGCCAGAAGATGCATCTGGAGGCTCCAGTCCTAGTGGGACTTCAAAGTCCGATGCCAACCGGGCAAG CAGTGGGGGAGGTGGAGGAGGCCTCATGGAAGAAATGAACAAGCTGCTGGCTAAGAGGAGAAAGGCAGCC TCCCAGACAGACAAGCCCGCTGACAGAAAGGAAGATGAGAGCCAAACGGAAGACCCTAGCACCTCCCCAT CCCCAGGTACCCGAGCCACCAGCCAGCCACCTAATTCCTCAGAGGCTGGCAGAAAACCCTGGGAACGGAG CAACTCGGTGGAGAAACCTGTGTCCTCGTTGCTGTCCAGGGTGAAGCCTGCTGGGAGTGTGAATGACGTG GGCCTGGATGCCTTAGATTTGGACCGGATGAAACAGGAGATCCTGGAGGAGGTGGTTCGGGAGCTGCACA AGGTGAAGGAGGAGATCATTGATGCCATCAGGCAGGAGCTAAGTGGGATCAGCACCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206142 representing NM_007965
Red=Cloning site Green=Tags(s) MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLK YNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSTQRQVQNGPSPEEMDIQRRQ VMEQQHRQESLERRISATGPILPPGHPSSAASTTLSCSGPPPPPPPPVPPPPTGSTPPPPPPLPAGGAQG TNHDESSASGLAAALAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAA SQTDKPADRKEDESQTEDPSTSPSPGTRATSQPPNSSEAGRKPWERSNSVEKPVSSLLSRVKPAGSVNDV GLDALDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007965 |
| ORF Size | 1179 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007965.3, NP_031991.3 |
| RefSeq Size | 2139 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 14026 |
| UniProt ID | P70429 |
| MW | 42.6 kDa |
| Gene Summary | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208460 | Evl (untagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MR206142L3 | Lenti ORF clone of Evl (Myc-DDK-tagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl), transcript variant 2 |
CNY 5,230.00 |
|
| MR206142L4 | Lenti ORF clone of Evl (mGFP-tagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl), transcript variant 2 |
CNY 5,230.00 |
