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Gfra3 (NM_010280) Mouse Tagged ORF Clone
CAT＃: MR206230
- TrueORF®
Gfra3 (Myc-DDK-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3)
"NM_010280" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GFRa; GFRalpha3; Y15110 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206230 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTCTCCTGGAGCCCGCGACCTCCACTGCTGATGATCCTGCTACTGGTGCTGTCGTTGTGGCTGC CACTTGGAGCAGGAAACTCCCTTGCCACAGAGAACAGGTTTGTGAACAGCTGTACCCAGGCCAGAAAGAA ATGCGAGGCTAATCCCGCTTGCAAGGCTGCCTACCAGCACCTGGGCTCCTGCACCTCCAGTTTAAGCAGG CCGCTGCCCTTAGAGGAGTCTGCCATGTCTGCAGACTGCCTAGAGGCAGCAGAACAACTCAGGAACAGCT CTCTGATAGACTGCAGGTGCCATCGGCGCATGAAGCACCAAGCTACCTGTCTGGACATTTATTGGACCGT TCACCCTGCCCGAAGCCTTGGTGACTACGAGTTGGATGTCTCACCCTATGAAGACACAGTGACCAGCAAA CCCTGGAAAATGAATCTTAGCAAGTTGAACATGCTCAAACCAGACTCGGACCTCTGCCTCAAATTTGCTA TGCTGTGTACTCTTCACGACAAGTGTGACCGCCTGCGCAAGGCCTACGGGGAGGCATGCTCAGGGATCCG CTGCCAGCGCCACCTCTGCCTAGCCCAGCTGCGCTCCTTCTTTGAGAAGGCAGCAGAGTCCCACGCTCAG GGTCTGCTGCTGTGTCCCTGTGCACCAGAAGATGCGGGCTGTGGGGAGAGGCGGCGTAACACCATCGCCC CCAGTTGCGCCCTGCCTTCTGTAACCCCCAATTGCCTGGATCTGCGGAGCTTCTGCCGTGCGGACCCTTT GTGCAGATCACGCCTGATGGACTTCCAGACCCACTGTCATCCTATGGACATCCTTGGGACTTGTGCAACT GAGCAGTCCAGATGTCTGCGGGCATACCTGGGGCTGATTGGGACTGCCATGACCCCAAACTTCATCAGCA AGGTCAACACTACTGTTGCCTTAAGCTGCTCCTGCCGAGGCAGCGGCAACCTACAGGACGAGTGTGAACA GCTGGAAAGGTCCTTCTCCCAGAACCCCTGCCTCGTGGAGGCCATTGCAGCTAAGATGCGTTTCCACAGA CAGCTCTTCTCCCAGGACTGGGCAGACTCTACTTTTTCAGTGGTGCAGCAGCAGAACAGCAACCCTGCTC TGAGACTGCAGCCCAGGCTACCCATTCTTTCTTTCTCCATCCTTCCCTTGATTCTGCTGCAGACCCTCTG G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206230 protein sequence
Red=Cloning site Green=Tags(s) MGLSWSPRPPLLMILLLVLSLWLPLGAGNSLATENRFVNSCTQARKKCEANPACKAAYQHLGSCTSSLSR PLPLEESAMSADCLEAAEQLRNSSLIDCRCHRRMKHQATCLDIYWTVHPARSLGDYELDVSPYEDTVTSK PWKMNLSKLNMLKPDSDLCLKFAMLCTLHDKCDRLRKAYGEACSGIRCQRHLCLAQLRSFFEKAAESHAQ GLLLCPCAPEDAGCGERRRNTIAPSCALPSVTPNCLDLRSFCRADPLCRSRLMDFQTHCHPMDILGTCAT EQSRCLRAYLGLIGTAMTPNFISKVNTTVALSCSCRGSGNLQDECEQLERSFSQNPCLVEAIAAKMRFHR QLFSQDWADSTFSVVQQQNSNPALRLQPRLPILSFSILPLILLQTLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010280 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010280.1, NM_010280.2, NM_010280.3, NM_010280.4, NP_034410.3 |
| RefSeq Size | 1992 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 14587 |
| UniProt ID | O35118 |
| MW | 44.3 kDa |
| Gene Summary | This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201928 | Gfra3 (untagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3), (10ug) |
CNY 3,656.00 |
|
| MR206230L3 | Lenti ORF clone of Gfra3 (Myc-DDK-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3) |
CNY 5,230.00 |
|
| MR206230L4 | Lenti ORF clone of Gfra3 (mGFP-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3) |
CNY 5,230.00 |
