Snx4 (BC050851) Mouse Tagged ORF Clone

CAT#: MR206319

  • TrueORF®

Snx4 (Myc-DDK-tagged) - Mouse sorting nexin 4 (cDNA clone MGC:59421 IMAGE:6512558)

ORF Plasmid: DDK tGFP



  "BC050851" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206319 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGGAAATAATTTTTGGTTGAAGAAAATAGAAATCAGTGTTTCAGAAGCAGAGAAGAGAACCGGAA
GGAACGCCGTGAACATGCAAGAAACGTACACTGCCTACCTCATCGAGACTCGGTCAGTTGAGCATGCCGA
TGGTCAGAGTGTGCTCACAGACTCGCTGTGGAGGCGGTACAGTGAGTTCGAGTTGTTGAGAAACTACCTT
CTAGTGTACTACCCACATGTTGTTGTGCCACCTCTCCCAGAAAAGCGGGCAGAGTTCGTGTGGCATAAAC
TCTCTGCTGACAACATGGACCCAGACTTTGTGGAGAGACGACGCGTGGGCTTAGAAAACTTCCTCTTGAG
GGTTGCTTCACATCCTGTCCTTTGTAGAGACAAAATCTTCTATTCATTTTTAACCCAGGAAGGTAACTGG
AAGGAGACTGTGAATGAGACTGGATTTCAGCTGAAGGCAGACTCCAGGTTAAAAGCGCTTAATGCAACAT
TCAGAGTGAAAAACCCAGACAAGAGGTTTACTGAGCTGAGGCACTACAGTGATGAGCTGCAGTCTGTCAT
CTCGCATCTCCTTCGAGTCAGAGCTAGAGTAGCAGATCGACTCTATGGTGTATATAAAGTACATGGGAAT
TATGGGAGAGTTTTTAGTGAATGGAGTGCCATCGAAAAAGAAATGGGGGATGGGCTGCAGAGTGCTGGGC
ATCACATGGACGTGTATGCATCTTCTATTGATGATATTTTGGAAGATGAAGAGCACTATGCAGATCAGCT
GAAGGAGTATCTGTTTTATGCAGAAGCACTTCGGGCTGTGTGCAGGAAGCATGAGCTTATGCAGTATGAC
CTGGAGACAGCTGCTCAAGACCTGGCTGCCAAGAAGCAGCAGTGCGAGGAGCTGGCCACCGGGACTGTGA
GAACATTCTCGTTGAAGGGAATGACTACCAAACTCTTTGGTCAAGAAACTCCAGAGCAAAGAGAAGCCAG
GATAAAGGTGCTAGAGGAGCAGATAAATGAAGGGGAACAGCAGCTGAAGTCTAAAAATCTGGAAGGCAGA
GAATTTGTGAAAAATGCATGGGCTGATATTGAACGCTTCAAAGAACAAAAGAACCGGGACCTAAAGGAAG
CTCTCATCAGCTATGCTGTCATGCAGATCAGCATGTGCAAAAAGGGAATTCAGGTTTGGACCAATGCTAA
AGAATGCTTCAGCAAGATG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR206319 protein sequence
Red=Cloning site Green=Tags(s)

MTGNNFWLKKIEISVSEAEKRTGRNAVNMQETYTAYLIETRSVEHADGQSVLTDSLWRRYSEFELLRNYL
LVYYPHVVVPPLPEKRAEFVWHKLSADNMDPDFVERRRVGLENFLLRVASHPVLCRDKIFYSFLTQEGNW
KETVNETGFQLKADSRLKALNATFRVKNPDKRFTELRHYSDELQSVISHLLRVRARVADRLYGVYKVHGN
YGRVFSEWSAIEKEMGDGLQSAGHHMDVYASSIDDILEDEEHYADQLKEYLFYAEALRAVCRKHELMQYD
LETAAQDLAAKKQQCEELATGTVRTFSLKGMTTKLFGQETPEQREARIKVLEEQINEGEQQLKSKNLEGR
EFVKNAWADIERFKEQKNRDLKEALISYAVMQISMCKKGIQVWTNAKECFSKM

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC050851
ORF Size 1209 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC050851
RefSeq Size 2699 bp
RefSeq ORF 1211 bp
Locus ID 69150
MW 47 kDa
Gene Summary Involved in the regulation of endocytosis and in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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