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Pdk3 (NM_145630) Mouse Tagged ORF Clone
CAT＃: MR206565
- TrueORF®
Pdk3 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_145630" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610001M10Rik; AI035637 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206565 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCTTCTACCGGCTGCTCAAGCAGCCGGTCCCCAAGCAGATCGAGCGCTACTCCCGCTTCTCCC CGTCGCCACTGTCTATCAAACAGTTCCTGGACTTCGGAAGGGATAATGCATGTGAAAAAACTTCCTATAT GTTTCTGCGCAAGGAACTTCCTGTGCGACTGGCTAACACCATGAGAGAGGTTAATCTCTTGCCGGATAAC TTGTTGAACCGCCCTTCCGTGGGATTAGTTCAGAGCTGGTACATGCAGAGCTTCCTTGAACTTTTAGAAT ATGAAAACAAGAGCCCAGAAGACCCACGAGTTTTGGATAACTTTCTCAACGTTCTGATAAATATCAGAAA CAGACACAATGATGTTGTTCCTACAATGGCCCAAGGCGTGATTGAGTACAAGGAAAAGTTCGGGTTTGAT CCGTTCATTAGCAGTAACATTCAATATTTCCTGGATCGGTTTTATACCAACCGCATTTCTTTCCGCATGC TTATTAACCAGCACACACTTCTGTTTGGTGGTGACACTAACCCTGCACATCCGAAACATATAGGGAGTAT CGACCCCACCTGTAATGTAGCTGATGTGGTTAAAGATGCATATGAAACAGCCAAGATGCTTTGTGAACAG TATTACCTGGTAGCTCCAGAGCTAGAAGTTGAAGAATTCAATGCCAAAGCGCCAAACAAACCCATTCAGG TAGTTTATGTGCCGTCACATCTGTTTCACATGCTATTTGAGCTGTTCAAGAACTCAATGAGGGCAACAGT TGAACTACATGAAGATAAAAAAGAAGGCTACCCAGCTGTTAAAACTCTCGTTACTCTGGGTAAAGAAGAC TTGTCCATTAAGATAAGTGACCTAGGTGGTGGAGTCCCACTTCGAAAAATAGACCGTCTTTTTAACTACA TGTACTCAACTGCTCCTCGTCCCAGCCTGGAGCCTACAAGAGCGGCGCCCTTGGCTGGATTTGGTTATGG CTTGCCAATTTCTCGTCTGTATGCCAGATATTTTCAGGGAGATCTAAAACTGTATTCCATGGAAGGAGTG GGTACCGATGCAGTCATTTATTTGAAGGCCCTTTCAAGTGAATCCTTTGAGAGGCTGCCAGTTTTCAATA AGTCTGCATGGCGCCATTACAAGACCACTCCTGAAGCTGATGACTGGAGCAATCCCAGCAGTGAACCAAG GGATGCATCAAAATACAAGGCTAAACAGGACAAGATCAAGAGTAATAGAACTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206565 protein sequence
Red=Cloning site Green=Tags(s) MRLFYRLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDN LLNRPSVGLVQSWYMQSFLELLEYENKSPEDPRVLDNFLNVLINIRNRHNDVVPTMAQGVIEYKEKFGFD PFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQ YYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHEDKKEGYPAVKTLVTLGKED LSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGV GTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQDKIKSNRTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145630 |
| ORF Size | 1248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145630.3 |
| RefSeq Size | 2000 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 236900 |
| UniProt ID | Q922H2 |
| MW | 47.9 kDa |
| Gene Summary | Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200607 | Pdk3 (untagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,656.00 |
|
| MG206565 | Pdk3 (tGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3) |
CNY 3,140.00 |
|
| MR206565L3 | Lenti ORF clone of Pdk3 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR206565L4 | Lenti ORF clone of Pdk3 (mGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
