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Rnf128 (NM_023270) Mouse Tagged ORF Clone
CAT＃: MR206827
- TrueORF®
Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128)
ORF Plasmid: tGFP
"NM_023270" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300002C13Rik; AI987883; GRAIL; Greul1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206827 representing NM_023270
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCGCCGCCCGGGATCGGGGTCTACTGCCGCGGCGGCTGCGGAGCTGCCCGGCTACTGGCTTGGT GCTTCCTTCTGGCTCTGAGTCCGCACGCGCCCGGTTCCCGGGGAGCCGAAGCCGTGTGGACCGCGTACCT CAACGTGTCCTGGCGGGTTCCGCACACCGGAGTGAACCGCACGGTGTGGGAGCTGAGCGAGGAGGGCGTG TACGGCCAGGACTCGCCGCTGGAGCCCGTCTCCGGGGTCCTGGTACCGCCCGACGGGCCCGGGGCGCTCA ACGCCTGTAACCCGCACACCAATTTCACGGTGCCCACGGTTTGGGGGAGCACGGTGCAAGTATCTTGGTT GGCCCTCATCCAACGCGGTGGAGGCTGCACCTTCGCGGACAAGATCCATCTGGCTTCAGAGAGAGGGGCT TCTGGAGCGGTCATCTTTAACTTCCCTGGGACCCGCAATGAGGTCATCCCCATGTCTCACCCGGGTGCTG GGGACATTGTTGCAATCATGATTGGCAATCTGAAAGGAACAAAAATTCTGCAGTCTATTCAAAGAGGCAT CCAAGTCACAATGGTCATCGAAGTAGGGAAAAAACATGGCCCTTGGGTGAATCATTATTCAATTTTCTTC GTTTCTGTGTCCTTTTTCATAATTACGGCAGCAACCGTGGGCTATTTCATCTTTTATTCTGCTCGAAGAT TACGAAATGCAAGAGCTCAAAGCAGGAAGCAGAGGCAGTTAAAGGCAGATGCTAAAAAAGCTATTGGAAA GCTTCAGCTGCGCACCTTGAAACAAGGAGACAAGGAAATTGGCCCTGATGGAGATAGCTGTGCTGTGTGC ATTGAGCTCTATAAGCCAAATGATTTGGTGCGCATCCTAACCTGCAATCATATTTTCCATAAGACATGTG TGGACCCGTGGCTTTTAGAACACAGGACTTGCCCCATGTGCAAGTGTGACATTCTCAAAGCTCTGGGAAT TGAGGTGGATGTTGAAGATGGATCAGTGTCTTTACAAGTTCCTGTTTCTAATGAAGCATCTAATACTGCC TCTCCCCATGAAGAGGACAGTCGCAGTGAGACTGCATCATCTGGATATGCTTCAGTACAAGGAGCAGATG AGCCACCTCTGGAGGAACATGCGCAGTCAGCAAATGAAAATCTACAGCTGGTAAACCATGAAGCAAATTC TGTGGCCGTGGATGTTGTTCCCCATGTTGACAACCCAACCTTTGAAGAAGATGAAACTCCTGATCAAGAG GCAGCTGTTCGGGAGATTAAATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206827 representing NM_023270
Red=Cloning site Green=Tags(s) MGPPPGIGVYCRGGCGAARLLAWCFLLALSPHAPGSRGAEAVWTAYLNVSWRVPHTGVNRTVWELSEEGV YGQDSPLEPVSGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLASERGA SGAVIFNFPGTRNEVIPMSHPGAGDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFF VSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGKLQLRTLKQGDKEIGPDGDSCAVC IELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEASNTA SPHEEDSRSETASSGYASVQGADEPPLEEHAQSANENLQLVNHEANSVAVDVVPHVDNPTFEEDETPDQE AAVREIKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023270 |
| ORF Size | 1284 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023270.5, NP_075759.3 |
| RefSeq Size | 2764 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 66889 |
| UniProt ID | Q9D304 |
| MW | 46.7 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201428 | Rnf128 (untagged) - Mouse ring finger protein 128 (Rnf128), (10ug) |
CNY 3,656.00 |
|
| MG206827 | Rnf128 (tGFP-tagged) - Mouse ring finger protein 128 (Rnf128) |
CNY 5,256.00 |
|
| MR206827L3 | Lenti ORF clone of Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128) |
CNY 4,750.00 |
|
| MR206827L4 | Lenti ORF clone of Rnf128 (mGFP-tagged) - Mouse ring finger protein 128 (Rnf128) |
CNY 4,750.00 |
